Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,229 +A 1.2% coding (111/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,2291.→A 1.2% 4.3 0.0 ‑0.1247coding (111/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (118/126):  total (119/128)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.64e-01

Only 100 of 272 total aligned reads displayed.

ACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396129‑1396329
                                                                                                     |                                                                                                    
 cTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTca                                                                                                      <  2:1811758/101‑2 (MQ=255)
  ttttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.Tg                                                                                                    <  2:2478569/101‑1 (MQ=255)
   tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.Tgg                                                                                                   >  1:3029040/1‑101 (MQ=255)
    ttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGc                                                                                                  >  1:2761999/1‑101 (MQ=255)
     tCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCt                                                                                                 >  1:3336901/1‑101 (MQ=255)
        tCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTc                                                                                              <  2:1927243/101‑1 (MQ=255)
         cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCg                                                                                             <  1:3674647/101‑1 (MQ=255)
            cTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTC.CGGCTGTCGCTc                                                                                          >  1:1711377/1‑101 (MQ=255)
             tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCAGGGTGTC.TGGCTGTCGCTcc                                                                                         <  1:3455641/101‑1 (MQ=255)
              ggCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTccc                                                                                        <  1:4949597/101‑1 (MQ=255)
                cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTccccc                                                                                      <  2:2723375/101‑1 (MQ=255)
                cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTccccc                                                                                      >  1:500605/1‑101 (MQ=255)
                  ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCcg                                                                                    >  1:201324/1‑101 (MQ=255)
                         gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGc                                                                             >  2:4027427/1‑101 (MQ=255)
                         gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGc                                                                             >  1:1323975/1‑101 (MQ=255)
                         gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCAGCGGGGGc                                                                             >  2:2576452/1‑101 (MQ=255)
                         gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCGGCGAAAAACGGCCATGCCTCCCGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGc                                                                             <  1:4518753/101‑1 (MQ=255)
                           cgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGt                                                                           >  2:4826975/1‑101 (MQ=255)
                           cgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGt                                                                           >  2:766285/1‑101 (MQ=255)
                             ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTgg                                                                         >  2:3078610/1‑101 (MQ=255)
                             ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:767385/101‑1 (MQ=255)
                               aCGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:4487606/101‑1 (MQ=255)
                               aCGCTGCGTCGCACCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAGCGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGCGGCGTGGAt                                                                       <  1:838723/101‑1 (MQ=255)
                                cGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:2924691/101‑1 (MQ=255)
                                cGCTGCGTCGCACCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:2442228/101‑1 (MQ=255)
                                 gcggCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGGG.GTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:4165079/98‑1 (MQ=2)
                                    gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  2:1656854/1‑101 (MQ=255)
                                    gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  1:3352184/1‑101 (MQ=255)
                                    gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCAGGGTGTC.TGGCTGTCGCTCCCACCGCGGGGGCGTGGATCGaga                                                                  >  2:4968186/1‑99 (MQ=255)
                                     cGTGGCGCCCCCCGGGGGGGCGTGGACCGAAACCCGGCGAAAAGCGGCCATGCCGCCGGGGGTC.TGGCTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:2159508/101‑1 (MQ=25)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                >  1:401871/1‑101 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                >  1:1000246/1‑101 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcag                                                               <  2:2774468/101‑1 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagc                                                              <  1:35589/101‑1 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagc                                                              <  1:543146/101‑1 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAAcagc                                                              >  1:1465253/1‑101 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagca                                                             <  2:792642/101‑1 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcagcag                                                            <  1:1824729/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGt                                                          >  1:565107/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGt                                                          >  1:316573/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCcgc                                                   <  1:1279282/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCCGCcgcg                                                  >  2:3751092/1‑101 (MQ=255)
                                                    gggggCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCcgcg                                                  >  2:3144826/1‑101 (MQ=255)
                                                        gagTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGccc                                              <  2:1631974/99‑1 (MQ=255)
                                                        gCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGccc                                              >  1:2383434/1‑101 (MQ=255)
                                                         cGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGcccc                                             <  2:1492693/101‑1 (MQ=255)
                                                         cGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCCCGGcccc                                             >  1:2105139/1‑101 (MQ=255)
                                                          gTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCg                                            >  2:3566958/1‑101 (MQ=255)
                                                           tGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACAGCAGGTCAGCCGCGGCCCCgg                                           >  1:167805/1‑101 (MQ=255)
                                                           tGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCgg                                           >  1:2690001/1‑101 (MQ=255)
                                                           tGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACAGCAGGCCAGCCGCGGACCCgt                                           >  2:2211489/1‑100 (MQ=255)
                                                            tgATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGt                                          <  2:126439/100‑1 (MQ=255)
                                                            ggATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGt                                          >  2:872342/1‑101 (MQ=255)
                                                            ggATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGt                                          <  2:115479/101‑1 (MQ=255)
                                                             gATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTg                                         <  1:2053982/101‑1 (MQ=255)
                                                             gATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTg                                         >  2:3231325/1‑101 (MQ=255)
                                                             gATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTg                                         >  1:2829946/1‑101 (MQ=255)
                                                               tCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGaa                                       >  2:3462193/1‑101 (MQ=255)
                                                               tCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGaa                                       <  1:4151442/101‑1 (MQ=255)
                                                               acGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGaa                                       <  1:2561411/100‑1 (MQ=255)
                                                                cGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAAc                                      <  1:3708142/101‑1 (MQ=255)
                                                                cGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGGGAAc                                      >  1:26385/1‑101 (MQ=255)
                                                                 gAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACt                                     <  1:1130276/101‑1 (MQ=255)
                                                                 gAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACt                                     >  2:3294757/1‑101 (MQ=255)
                                                                   aaCCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTc                                   >  1:2469046/1‑101 (MQ=255)
                                                                   aaCCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTc                                   <  1:946085/101‑1 (MQ=255)
                                                                   aaCCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTc                                   <  2:3250841/101‑1 (MQ=255)
                                                                      ccGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGt                                <  2:3400204/101‑1 (MQ=255)
                                                                       cGGCGAAAACCGGCGATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGtt                               <  2:4466463/101‑1 (MQ=255)
                                                                        ggCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTtc                              <  2:3883364/101‑1 (MQ=255)
                                                                        ggCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTtc                              <  1:3022133/101‑1 (MQ=255)
                                                                        ggCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTtc                              <  2:3018799/101‑1 (MQ=255)
                                                                          cGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTtcgt                            >  1:4825443/1‑101 (MQ=255)
                                                                            aaaaaCGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCAGGTCAGCCGCGGCCCCGGGGAACTTCTCTtcgtgg                          >  1:4708521/1‑99 (MQ=255)
                                                                            aaaaaCGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTtcgtcg                          >  1:1559220/1‑101 (MQ=255)
                                                                                aCGGCCCTGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTcc                      <  2:167789/101‑1 (MQ=255)
                                                                                  ggCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTcccc                    >  2:2641241/1‑101 (MQ=255)
                                                                                    ccATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTcccccc                  <  2:1375349/101‑1 (MQ=255)
                                                                                    ccATGCCTCCGGGTGTC.TGGCTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTcccccc                  <  2:2104991/101‑1 (MQ=255)
                                                                                    ccATGCCTCCGGGTGTC.GGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTcccccc                  <  2:5053248/101‑1 (MQ=255)
                                                                                     cATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGCGCACTTCTGTTCGTCGCTCCCCCcg                 >  2:3704631/1‑101 (MQ=255)
                                                                                       tGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCcgcg               <  1:4880607/101‑1 (MQ=255)
                                                                                       tGCCTCCGGGTGTC.CGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCcgcg               >  2:4988571/1‑101 (MQ=255)
                                                                                          cTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCgggg            <  1:104948/101‑1 (MQ=255)
                                                                                          cTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCgggg            <  2:2754959/101‑1 (MQ=255)
                                                                                           tCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCggggg           >  1:4092657/1‑101 (MQ=255)
                                                                                            ccGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGc          >  2:4700120/1‑101 (MQ=255)
                                                                                            ccGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGc          >  2:4133811/1‑101 (MQ=255)
                                                                                            ccGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGCGAACTTCTGTTCGTCGCGCCCCCCGCgggggg          >  2:700424/1‑100 (MQ=255)
                                                                                              gggTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGGCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCgg        >  2:1864934/1‑100 (MQ=255)
                                                                                              gggTGTC.TGGCTGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGt        <  1:43836/101‑1 (MQ=255)
                                                                                                 tgtC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCCGTTCGTCGCTCCCCCCGCGGGGGCGTggc     >  2:2027804/1‑100 (MQ=255)
                                                                                                 gggc.TGGCTGTCGCACCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:3355813/98‑1 (MQ=255)
                                                                                                  gtC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:2245532/101‑1 (MQ=255)
                                                                                                  gtC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGAt    >  1:3835901/1‑101 (MQ=255)
                                                                                                  gtC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:4833715/101‑1 (MQ=255)
                                                                                                   tC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  2:3834892/101‑1 (MQ=255)
                                                                                                   tC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  2:2325802/101‑1 (MQ=255)
                                                                                                    c.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:4217370/101‑1 (MQ=255)
                                                                                                    c.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATCg  >  1:3082776/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
ACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTC.TGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCAGGTCAGCCGCGGCCCCGGTGAACTTCTGTTCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396129‑1396329

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG