BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,935	+T	7.1%	coding (817/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,935	1	.→T	7.1%	113.2 0.0 ‑0.6	424	coding (817/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (15/15): ref base (203/191): total (218/206)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.31e-01

Only 100 of 455 total aligned reads displayed.


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035


ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.a                                                                                                                    <  2:1492449/101‑1 (MQ=255)


 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..g                                                                                                                 >  2:331455/1‑101 (MQ=255)


  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..Gc                                                                                                                >  1:3512839/1‑101 (MQ=255)


    gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCt                                                                                                              >  1:573707/1‑101 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.g                                                                                                            <  1:1041585/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.g                                                                                                            <  2:813462/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.g                                                                                                            >  2:4559144/1‑101 (MQ=255)


      cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.gc                                                                                                           <  2:3217378/100‑1 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.gcg                                                                                                          <  2:17613/101‑1 (MQ=255)


               gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCa                                                                                                >  2:3626967/1‑101 (MQ=255)


                ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.t                                                                                              <  1:4668657/101‑1 (MQ=255)


                      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                      >  2:958840/1‑101 (MQ=255)


                      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                      >  1:4414915/1‑101 (MQ=255)


                       ggATCGAACCCGGTCCAGAGATAGGCCGCGNGGTCCCCCTAAGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                     <  1:1424647/101‑1 (MQ=37)


                           cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGc.c                                                                               >  1:4975406/1‑101 (MQ=255)


                           cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGc.c                                                                               <  1:3593500/101‑1 (MQ=255)


                            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGc.cc                                                                              >  1:1732799/1‑100 (MQ=255)


                            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.Ct                                                                              <  2:658157/101‑1 (MQ=255)


                            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.Ct                                                                              >  2:1694137/1‑101 (MQ=255)


                            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGGCGCTCCTCCCGCGGGGACGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.Ct                                                                              >  2:1654952/1‑101 (MQ=255)


                              aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..c                                                                          >  1:4145564/1‑101 (MQ=255)


                                   gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CCG..CG.TG.Gt                                                                   >  1:2327877/1‑101 (MQ=255)


                                     ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TG.CGC.CCG..CG.TG.GTCg                                                                 >  2:2844613/1‑101 (MQ=255)


                                     ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCg                                                                 >  2:2677452/1‑101 (MQ=255)


                                       agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCt                                                               >  1:1299497/1‑101 (MQ=255)


                                       agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCt                                                               <  2:3512569/101‑1 (MQ=255)


                                        gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTc                                                              >  2:1116184/1‑101 (MQ=255)


                                         agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTcc                                                             >  2:1233636/1‑101 (MQ=255)


                                          gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CCG..CG.Gg.ggccccccc                                                            >  1:3424814/1‑93 (MQ=37)


                                             agcccGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGTGGGCGTGGATCGAAAC.A..GCCG.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTgccccc                                                         <  2:225060/98‑1 (MQ=38)


                                             aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTcccccc                                                         <  2:3374768/101‑1 (MQ=255)


                                              gACCGCGCGGACCTCCTCAGACGTCGCTCCCGCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcg                                                        >  1:4485513/1‑101 (MQ=255)


                                               cccGCGCGGACCTCCTCAGGCGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCGCCCGCcgc                                                       <  2:1878481/100‑1 (MQ=38)


                                                ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcgcg                                                      >  2:4995430/1‑101 (MQ=255)


                                                       gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCgg                                               >  2:2315217/1‑100 (MQ=255)


                                                          tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:409452/92‑1 (MQ=0)


                                                          ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:1474956/91‑1 (MQ=9)


                                                          ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:2808515/101‑1 (MQ=255)


                                                           tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGAt                                           >  1:1894328/1‑101 (MQ=255)


                                                           tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCCTgggt                                           >  1:4765541/1‑99 (MQ=255)


                                                            tggccccgggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:29207/91‑1 (MQ=2)


                                                            tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:1945509/9‑101 (MQ=2)


                                                            tcaccactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:1344756/93‑1 (MQ=9)


                                                            gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:2637875/8‑101 (MQ=2)


                                                            cctcgcaCGTCGGCCCCCCCGCCGGGGCGTGGATGGAAGCAA..TGCG.GCGCG.GG.CCCA.T.GG.CT.GC.CGG.ACC..CGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:4801567/95‑1 (MQ=0)


                                                            cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCCCCCCCCGCGGGGGCGTGGATc                                          <  1:2874552/101‑1 (MQ=255)


                                                             cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:3873688/7‑101 (MQ=2)


                                                             caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGGTc                                          >  2:1986370/8‑101 (MQ=9)


                                                              tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGATCGa                                        >  1:3802797/1‑101 (MQ=255)


                                                              ccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:2218559/100‑1 (MQ=255)


                                                              aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:4290267/7‑101 (MQ=9)


                                                              aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCCCCCCCCGCGGGGGGGGGGGTCg                                         >  1:3902051/7‑101 (MQ=2)


                                                               cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                       >  1:635910/1‑101 (MQ=255)


                                                                gccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:4277650/5‑101 (MQ=2)


                                                                gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:4175570/4‑101 (MQ=2)


                                                                cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:903933/97‑1 (MQ=2)


                                                                aggccccGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:2260256/95‑1 (MQ=255)


                                                                 tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:180527/99‑1 (MQ=2)


                                                                 tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:290430/3‑101 (MQ=2)


                                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTTGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                     >  1:747289/1‑101 (MQ=255)


                                                                 gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  1:3771865/101‑1 (MQ=255)


                                                                  ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  2:312723/3‑101 (MQ=9)


                                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    >  2:3058242/3‑100 (MQ=2)


                                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                   <  1:2001456/100‑3 (MQ=2)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                   <  2:1090364/101‑3 (MQ=2)


                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                   <  1:989258/101‑3 (MQ=9)


                                                                    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..CCCT.GCGCC.GC.AGCG.AGTG.CG.TCTGGA.ACA..GG.AT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                   <  2:4434227/101‑3 (MQ=2)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    <  2:3735872/101‑1 (MQ=2)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 >  1:4860951/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 <  1:2667094/101‑1 (MQ=255)


                                                                     gcGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCa                                 <  2:2129897/100‑1 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                >  2:2427233/1‑101 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAActaaac                                >  2:4009691/1‑96 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                 >  1:3678483/1‑97 (MQ=9)


                                                                       gCTCCCCCCGCGGGGGCGTGGATTGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGATCCCCCCGCGGGGGGGTGGTTCGAAACATAAGc                               >  1:4064415/1‑101 (MQ=37)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                  >  1:4938516/1‑99 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGACTATG.CGC.TGGA.CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccacacc                               >  1:1638978/1‑95 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               >  2:4741744/1‑95 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  2:1447142/101‑7 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  1:2155961/101‑7 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                >  1:4218476/1‑96 (MQ=9)


                                                                        ccccccccGCGGGGGCGTGGGTCCAAAC.ATTGCTT.TGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                               <  2:2766090/99‑7 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCTCCGCGGGGGCGTGGATCGAAACATCAGCt                              <  2:468931/101‑1 (MQ=255)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                             >  1:322135/1‑93 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                               <  1:313218/101‑3 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAG.GT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAccccccacc                             >  2:1299302/1‑93 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGG.CC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                            >  2:2969986/1‑92 (MQ=2)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                             <  1:3033146/101‑9 (MQ=9)


                                                                           cccccGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                           <  1:1502598/101‑1 (MQ=255)


                                                                           cccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                            <  2:4921676/101‑10 (MQ=9)


                                                                           cccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGG.ATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                            <  1:2414911/101‑10 (MQ=9)


                                                                              ccGGGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                        <  1:682592/101‑1 (MQ=255)


                                                                              ccGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCAT.CG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                           <  1:1141240/101‑7 (MQ=0)


                                                                               cgcgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                       <  1:3333269/101‑1 (MQ=255)


                                                                               cgcgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                       <  1:210884/101‑1 (MQ=255)


                                                                                  gggggCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                    >  1:4004196/1‑101 (MQ=255)


                                                                                       ggTGGATAGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc               <  2:747199/100‑1 (MQ=255)


                                                                                        gTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGGTGGGCAGCCg              >  2:4335797/1‑101 (MQ=255)


                                                                                          ggATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa            >  2:1399203/1‑101 (MQ=255)


                                                                                                   c.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg   <  1:2595785/101‑1 (MQ=255)


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGG.CT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG