Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,563 +C 1.8% coding (1445/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5631.→C 1.8% 6.9 0.0 ‑0.1273coding (1445/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (135/133):  total (138/135)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.74e-01

Only 100 of 314 total aligned reads displayed.

AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663
                                                                                                                                                                                                             
 acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCta                                                                                                     >  1:1481194/1‑100 (MQ=255)
 acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTg                                                                                                     <  2:1581781/101‑1 (MQ=255)
      gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTGTTCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTc                                                                                                >  1:3494110/1‑101 (MQ=37)
      gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTc                                                                                                <  2:1067109/101‑1 (MQ=255)
       tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTcc                                                                                               >  1:137353/1‑100 (MQ=255)
           caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                                           >  1:201059/1‑101 (MQ=255)
            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTccc                                                                                          >  2:4454991/1‑101 (MQ=255)
            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTccc                                                                                          >  1:1000175/1‑101 (MQ=255)
                 gCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                     <  2:786732/101‑1 (MQ=255)
                 gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                     >  1:1849373/1‑101 (MQ=255)
                 gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                     >  1:498381/1‑101 (MQ=255)
                 ccAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                     <  2:109327/100‑1 (MQ=255)
                    aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCTTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                                                                                  >  1:4354573/1‑101 (MQ=255)
                    aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                                                                                  >  2:323002/1‑101 (MQ=255)
                         ccTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                                             <  2:3310703/101‑1 (MQ=255)
                         ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                                             >  2:3485924/1‑101 (MQ=255)
                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  2:3127992/101‑1 (MQ=255)
                           ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGA.C.C..CAGTTCA.CGGCGGGCTCTCGTCGCCCCCCCCCCGGGGGCGTGGa                                                                          >  1:635273/11‑101 (MQ=0)
                              accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.A..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:591651/101‑1 (MQ=255)
                              acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGGGGGTCg                                                                       >  1:2152197/8‑101 (MQ=0)
                               cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       >  1:3940410/7‑101 (MQ=2)
                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:1244156/101‑1 (MQ=255)
                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:4023952/101‑1 (MQ=255)
                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  2:59962/101‑1 (MQ=255)
                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  2:1222346/101‑1 (MQ=255)
                                cgagcgTCTCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:4184528/96‑1 (MQ=0)
                                caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      <  2:3088297/101‑1 (MQ=255)
                                aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  1:4619179/96‑1 (MQ=2)
                                 gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      >  1:4539078/5‑101 (MQ=2)
                                 ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  2:4694280/5‑101 (MQ=2)
                                 accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  2:3196296/1‑101 (MQ=255)
                                  tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  1:3873980/98‑1 (MQ=2)
                                  ccGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  2:4332163/101‑1 (MQ=255)
                                   cGGTCGCTCCCCCGGGGGGGGCGTGGATCGAAACAA.CTGACCCGGGAA.C..GGCCTGTGCGCTG.GACGTGTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                  <  2:1815864/101‑2 (MQ=2)
                                   atgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                                  <  2:3997987/99‑2 (MQ=2)
                                    gggcGCTCCCCCCGGGGGGGCGTCGATCGAAACACCCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                  <  2:1481064/98‑1 (MQ=37)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  2:2942388/100‑1 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  1:26221/1‑101 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  2:3101903/1‑101 (MQ=255)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 >  2:722121/1‑101 (MQ=255)
                                     gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 <  1:2403980/101‑1 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                <  1:2101766/101‑1 (MQ=2)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                <  1:3137738/101‑1 (MQ=2)
                                      gcGCTCCCCCCGCTGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                <  2:2507836/100‑1 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                              <  2:2562468/101‑6 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAAcggggg                                                              >  2:2184247/1‑96 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                               >  1:3557851/1‑101 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                             <  2:4884037/101‑7 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                             <  1:4200446/101‑7 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                               <  1:709721/101‑4 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                               >  1:4126686/1‑98 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                              >  1:1948376/1‑101 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                            <  2:417589/101‑5 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                            <  1:1421333/101‑1 (MQ=255)
                                          cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                           <  2:4572235/100‑9 (MQ=2)
                                          cccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                             <  1:2815117/100‑6 (MQ=0)
                                           ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                          <  2:4315229/101‑10 (MQ=2)
                                           ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                            >  2:828414/1‑95 (MQ=0)
                                            cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                                         <  1:1663391/101‑11 (MQ=0)
                                             ccccGGGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                         <  2:3823277/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGAt                                                         >  2:222497/1‑101 (MQ=255)
                                              cccGCGGGGGCGTGGGTCGAAACAACCCGATCATGG.C.C..CGCCCCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                        >  1:380968/1‑101 (MQ=255)
                                               ccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                                       >  1:4919977/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                    >  2:3915795/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                   <  1:1091230/101‑1 (MQ=255)
                                                     gggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                                 >  1:1054237/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                >  1:1775533/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                >  2:1533238/1‑101 (MQ=255)
                                                          tGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                            >  2:1904895/1‑101 (MQ=255)
                                                          tGGATCAAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                            <  1:829236/101‑1 (MQ=255)
                                                           ggATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAgccgcc                                           >  2:164977/1‑101 (MQ=255)
                                                            gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          <  1:3221149/101‑1 (MQ=255)
                                                            gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          >  1:3286043/1‑101 (MQ=255)
                                                            gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                          >  1:4855283/1‑101 (MQ=255)
                                                             agcGAAACAACCCGCTCCGGG.C.C..CGCCTCAGCGCCGCGCTGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                         <  2:5000046/99‑1 (MQ=37)
                                                              tCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                        <  2:3610050/101‑1 (MQ=255)
                                                                gAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                      >  1:2877629/1‑101 (MQ=255)
                                                                 aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGTCGTGACCcgccccgccc                                     >  1:2490926/1‑101 (MQ=255)
                                                                 aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                     >  2:2347835/1‑101 (MQ=255)
                                                                 aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                     >  2:559564/1‑101 (MQ=255)
                                                                 aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                     <  1:2457202/101‑1 (MQ=255)
                                                                   acaccCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                                   <  2:4354256/101‑1 (MQ=255)
                                                                        ccGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                              <  2:2384011/101‑1 (MQ=255)
                                                                            tCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                          >  1:2363042/1‑101 (MQ=255)
                                                                             cttGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGTGGTGGATAGAAACATCCGCGGATCGGGGTGACCCGCCCCGCCCATCGGTCGCTcc                         <  1:3383903/99‑1 (MQ=38)
                                                                              aggg.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGTATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                        <  1:2214594/99‑1 (MQ=255)
                                                                                gg.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGCCGCTccccc                      >  2:1001806/1‑101 (MQ=255)
                                                                                 g.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                     <  1:4312641/101‑1 (MQ=255)
                                                                                     c..cGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc                   <  1:3714466/101‑1 (MQ=255)
                                                                                            ccAGCGCCGCTGTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg              <  2:3719039/100‑1 (MQ=255)
                                                                                                 gccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg         <  1:3102141/101‑1 (MQ=255)
                                                                                                  ccgcGCTGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa        <  1:4257497/101‑1 (MQ=255)
                                                                                                  ccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa        <  2:2877416/101‑1 (MQ=255)
                                                                                                  ccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa        <  1:4424354/101‑1 (MQ=255)
                                                                                                    gcgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      >  1:1535263/1‑101 (MQ=255)
                                                                                                    gcgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc      <  1:3056722/101‑1 (MQ=255)
                                                                                                     cgcTGACGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  2:1775403/101‑1 (MQ=255)
                                                                                                      gcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa    >  2:3554424/1‑101 (MQ=255)
                                                                                                      gcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGGGGATCGa    >  1:4737577/1‑101 (MQ=255)
                                                                                                        tGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  <  1:2672264/101‑1 (MQ=255)
                                                                                                                                                                                                             
AACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397463‑1397663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG