Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,702	+GG	100%	intergenic (‑126/‑277)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,702	1	.→G	100.0%	137.9	55	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/53):  ref base (1/0):  total (1/54)
*	NC_014034	1,732,702	2	.→G	100.0%	142.4	55	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/54):  ref base (1/0):  total (1/54)

TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732604‑1732784                                                                                                         ||                                                                                        tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..g                                                                                        >  2:2310360/1‑101 (MQ=255)  atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCGGC.Cc...gggc..gg                                                                                       >  2:4490965/1‑101 (MQ=255)  atataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cc...cggg..ggg                                                                                      >  2:1567708/1‑101 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggg                                                                                     <  1:3930975/101‑2 (MQ=255)     taGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGGGGG.GA.Cc...cggg..gggggg                                                                                   >  2:2893054/1‑101 (MQ=255)      aGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGGCGCGGC.GG.CC...CGG...GGGCgg..g..g                                                                             >  1:4382640/1‑101 (MQ=255)       gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggct                                                                                  <  2:1819975/101‑5 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GG.CC...CCCC..GGGGgg..g..gg                                                                            >  1:1904076/1‑100 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctc                                                                                 <  1:3803435/101‑6 (MQ=255)         gTATCGGGGGGAGGGGTAAAGGCCGGCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctcc                                                                                <  1:1072471/101‑7 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGG.NA.Cc...cggg..ggnngnnnnn                                                                               >  2:2127742/1‑96 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctcc                                                                                <  2:597974/101‑7 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..CGGCgg..g..gg                                                                            >  1:2230135/1‑100 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GCGGgg..g..gg                                                                            >  2:3702518/1‑100 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...CGCC..GCGCcg..g..gtc                                                                           >  2:4882319/1‑99 (MQ=255)          tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggctccg                                                                               <  2:28758/101‑8 (MQ=39)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GG.CC...CGGG..GCGGGG..G..Gcccc                                                                         >  1:1329482/1‑101 (MQ=255)             cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCA.GGGCGG..G..Gcccc                                                                         >  2:1286185/1‑101 (MQ=255)             cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGC..G..Gcgccc                                                                        >  2:616588/1‑101 (MQ=255)              ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGGGGC.CA.CC...CGGG..GGGGgg..g..ggggcccc                                                                      >  1:1680076/1‑94 (MQ=255)               gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCc...gggc..gggggg..gcgcgcccc                                                                        >  2:3999604/1‑93 (MQ=255)               gggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..G..Ggccgccc                                                                      >  1:4114447/1‑97 (MQ=255)                ggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..CGGGGG..G..Gcgcccccc                                                                     >  2:1606560/1‑98 (MQ=255)                 gggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..T..Cccccccccc                                                                    >  2:744264/1‑97 (MQ=255)                   gagaGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..GGGGGG..G..GGCCGCCCAccc                                                                  >  2:4377324/1‑101 (MQ=255)                    agagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...CGGG..GGGGGG..G..GGCCCCCCCccccc                                                                >  1:2287093/1‑101 (MQ=255)                        gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCg                                                               <  1:2128023/101‑1 (MQ=25)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGG..CGGGGG..G..GCGGCCGCCccccccccc                                                            >  1:1389059/1‑97 (MQ=255)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                              <  2:3261251/101‑1 (MQ=25)                         tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCgg                                                              <  2:1307984/101‑1 (MQ=25)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGc                                                             <  2:1701662/101‑1 (MQ=25)                            ccgatctCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  1:3391033/95‑1 (MQ=21)                            aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCCCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  2:1388472/101‑1 (MQ=25)                            aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTCCCAAGCAGGGTTTCGGCGCGGCGGGGCCGCGGC.GACCC...GGGCCGGGGCTC..C..GCCCCCGCACCCCCGGCGt                                                           <  2:4172312/101‑1 (MQ=25)                             ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTa                                                          <  1:1317713/101‑1 (MQ=25)                              gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  2:4382323/101‑1 (MQ=25)                              gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  2:4494322/101‑1 (MQ=25)                              gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.t                                                        <  1:3702860/101‑1 (MQ=25)                               ccGGCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.tt                                                       <  2:2110618/101‑1 (MQ=21)                                cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.ttt                                                      <  2:2340733/101‑1 (MQ=25)                                cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.ttt                                                      <  2:968515/101‑1 (MQ=25)                                 gTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTg                                                     <  2:1488043/101‑1 (MQ=21)                                      tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCa                                                <  1:4094346/101‑1 (MQ=21)                                      tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCa                                                <  2:525119/101‑1 (MQ=21)                                        cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaag                                              <  1:1766103/101‑1 (MQ=21)                                         ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaaga                                             <  2:2583877/101‑1 (MQ=21)                                         ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaaga                                             <  1:2498349/101‑1 (MQ=21)                                           ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCGCCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:3395357/101‑1 (MQ=18)                                           ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  2:1643264/101‑1 (MQ=18)                                           ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:3776589/101‑1 (MQ=18)                                           ggCGGAAAGCCGCGTGCCAAGCAGGGTTGCGCCACGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCaagaag                                           <  1:4377641/101‑1 (MQ=18)                                            gCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAagaaga                                          <  2:4739715/101‑1 (MQ=18)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAgaagaa                                         <  2:3221040/101‑1 (MQ=18)                                              ggAAAGCCGCGTGCAAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCGCGGCGTA.TTTGCGCCAAGAAGaaa                                        <  2:2504440/101‑1 (MQ=18)                                                aaaGCCGCGTGCGAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACa                                      <  1:4375377/101‑1 (MQ=18)                                                 aaGCGGCGTGCCAGGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACaa                                     <  1:3447389/101‑1 (MQ=17)                                                 aaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGC..GGGGGG..GCCGCGCCCGCACCCCCCGCGCA.TTTTTGACAAGAAGAAAAaa                                     >  2:3089241/1‑101 (MQ=255)                                                   gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGc                                   <  2:4592591/101‑1 (MQ=18)                                                   gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGc                                   <  1:4920762/101‑1 (MQ=18)                                                      gcgTGGCAAGCAGGGTTTCGCGGGGGCGGGGCGGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCggg                                <  1:1029493/101‑1 (MQ=17)                                                      gcgTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCTGCGTA.TTTGCGCCAAGAAGAAACAAGCggg                                <  1:4491285/101‑1 (MQ=18)                                                        gggCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAc                              <  2:83904/99‑1 (MQ=18)                                                        gTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CCCGGGGGC..GGGGGG..G..CCCCCCCCACCCCCCGCGCATTTTTCGCC.AGAAAAAACaaaagggga                              >  2:2322397/1‑94 (MQ=255)                                                         tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  2:3229039/101‑1 (MQ=18)                                                         tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  1:3831048/101‑1 (MQ=18)                                                         tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGAcc                             <  1:65293/101‑1 (MQ=18)                                                          gCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCa                            <  2:4697739/101‑1 (MQ=18)                                                           ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:1042902/101‑1 (MQ=18)                                                           ccAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAg                           <  2:4924579/101‑1 (MQ=18)                                                            cAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAgg                          <  2:4750756/101‑1 (MQ=18)                                                              aGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcc                        <  1:1477665/101‑1 (MQ=18)                                                                cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  2:2592945/101‑1 (MQ=18)                                                                cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  1:1411749/101‑1 (MQ=18)                                                                cAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccc                      <  2:3097491/101‑1 (MQ=18)                                                                      ttCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa                <  1:75397/101‑1 (MQ=18)                                                                       tCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAg               <  1:1762962/101‑1 (MQ=18)                                                                       tCGCCGCGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAg               <  1:114457/101‑1 (MQ=18)                                                                        cgccgcGGCGGGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGc              <  1:257664/101‑1 (MQ=18)                                                                             cggcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt         <  2:462330/101‑1 (MQ=18)                                                                               ggggggCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  1:4261380/99‑1 (MQ=18)                                                                               gcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  2:254259/101‑1 (MQ=18)                                                                               gcggGGCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc       <  1:1327336/101‑1 (MQ=18)                                                                                   ggCCGCGGC.GACCC...GGGC..GGGGGGTCC..CCCCCCCCCCCCCCCGCGGA.TTTTCGCCCAGAAGAAAACAACGGGACCAGGCCCCCCCGCAGCCCCACGCCCTc   >  2:871571/1‑101 (MQ=255)                                                                                    gCCGCGGC.GACCC...GGGCGGGGGCTC..C..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTcc  <  2:2286929/101‑1 (MQ=18)                                                                                                         ||                                                                                        TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC...GGGC..GGGCGG..G..GCCCCCGCACCCCCGGCGTA.TTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCCGTCC  >  NC_014034/1732604‑1732784 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG