BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,745,454	+C	100%	intergenic (‑114/+654)	RCAP_rcc01613 ← / ← metH4	hypothetical protein/methionine synthase subunit B

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,745,454	1	.→C	100.0%	64.0	147	intergenic (‑114/+654)	RCAP_rcc01613/metH4	hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand): new base (89/37): ref base (5/16): total (94/53)
Fisher's exact test strand distribution p-value = 9.81e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.46e-02

Only 100 of 190 total aligned reads displayed.


CAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACG.GCGCGAGCTTTGGCACCT.C.A..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCAAGGCGCCCGG  >  NC_014034/1745355‑1745553


cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..c                                                                                                      >  1:4565978/1‑101 (MQ=255)


 aGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCTGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cc                                                                                                     <  2:3768593/101‑1 (MQ=255)


  gcgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..ccc                                                                                                    <  2:4491710/101‑1 (MQ=255)


   cgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cccc                                                                                                   <  2:58270/101‑1 (MQ=255)


   cgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCCCGAGC.TTGGCCCCC.C.A..cccc                                                                                                   >  1:3458480/1‑101 (MQ=255)


   cgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGACGCGCGCGAGC.TTGGCACCT.C.A..cccc                                                                                                   >  2:3492068/1‑101 (MQ=255)


   ccccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cccc                                                                                                   <  1:3683190/99‑1 (MQ=255)


     ccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cccccc                                                                                                 <  2:733369/101‑1 (MQ=255)


      cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..ccccccc                                                                                                >  1:145217/1‑101 (MQ=255)


       ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cccccccc                                                                                               <  1:2912693/101‑1 (MQ=255)


         ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..cccccccccg                                                                                             >  2:2683103/1‑99 (MQ=255)


             cggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..ccccccccccgcgc                                                                                         >  2:3314341/1‑95 (MQ=255)


              ggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCa                                                                                         >  2:2580126/1‑101 (MQ=255)


               gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..ccccccccccgcgcac                                                                                       >  1:3068203/1‑93 (MQ=255)


                ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCAcc                                                                                       <  2:2096439/101‑1 (MQ=255)


                ggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..ccccccccccgcgcccc                                                                                      >  2:1825857/1‑92 (MQ=255)


                 gggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCg                                                                                      <  2:4982127/101‑1 (MQ=255)


                   gcgggggTCGGCAGCCCGGCCGTCGGATCGGCCACCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGc                                                                                    <  1:4337708/101‑1 (MQ=255)


                    cgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCACCGGcc                                                                                   >  1:3801007/1‑100 (MQ=255)


                    cgggggGCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCC.CAA..CCCACCCCCGCGCACCGGca                                                                                   >  2:3431566/1‑101 (MQ=255)


                     gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGcac                                                                                  <  1:521845/101‑1 (MQ=255)


                     gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCCCCGGcac                                                                                  >  1:2289977/1‑101 (MQ=255)


                     gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCACCGGcac                                                                                  >  1:1120295/1‑101 (MQ=255)


                      ggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGcaca                                                                                 <  1:3930595/101‑1 (MQ=255)


                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCag                                                                            <  1:3897113/101‑1 (MQ=255)


                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.A..CCCCCCCCGGCGCACCGGCACAGGCaga                                                                           >  2:2512007/1‑101 (MQ=255)


                             gCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCagag                                                                          >  1:1786947/1‑101 (MQ=255)


                              cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGGCCCCCCGCACAGCCagaga                                                                         >  2:3804772/1‑101 (MQ=255)


                                gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAgc                                                                       <  1:4689008/101‑1 (MQ=255)


                                 cccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCCCCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcg                                                                      <  1:4844624/101‑1 (MQ=255)


                                 cacgcccGCCGGATCGGCCAGAGGCATGTCGATCGCGGCGGTCGACGCGCGCGAG.ATTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcg                                                                      <  2:4846770/96‑1 (MQ=255)


                                  ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                                     <  1:1228604/101‑1 (MQ=255)


                                      ccGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggcg                                                                 <  1:206279/101‑1 (MQ=255)


                                      ccGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggcg                                                                 <  1:660954/101‑1 (MQ=255)


                                        gTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACCGCCAGAGAGCGCggcggc                                                               >  1:4416498/1‑101 (MQ=255)


                                         tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcc                                                              >  2:1957826/1‑101 (MQ=255)


                                         tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcc                                                              >  1:2065096/1‑101 (MQ=255)


                                          cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCCCAGCCCGAGAGCCCGGCGGCCa                                                             >  2:2919774/1‑101 (MQ=255)


                                           ggATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcccg                                                            >  2:3483225/1‑99 (MQ=255)


                                            gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                           <  1:2683288/101‑1 (MQ=255)


                                            gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                           >  2:3090912/1‑101 (MQ=255)


                                            gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                           >  2:966344/1‑101 (MQ=255)


                                               cGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                        <  2:2616570/101‑1 (MQ=255)


                                               cGGCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                        <  1:2353328/101‑1 (MQ=255)


                                                ggCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                       <  1:3016148/101‑1 (MQ=255)


                                                ggCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcca                                                       >  2:4234174/1‑101 (MQ=255)


                                                 gCCAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                      >  1:2215965/1‑101 (MQ=255)


                                                 gCCAGCGGCATATCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                      <  1:4965805/101‑1 (MQ=255)


                                                  ccAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagc                                                     <  2:5038242/101‑1 (MQ=255)


                                                  ccAGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagc                                                     <  1:1660389/101‑1 (MQ=255)


                                                    aGCGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGccc                                                   <  1:2257046/101‑1 (MQ=255)


                                                      cGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                                 <  2:2414448/101‑1 (MQ=255)


                                                      cGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACGGGGACACCCAGAGAGCGCGGCGGCCAGACTCAGCCCCg                                                 >  2:2847939/1‑101 (MQ=255)


                                                      cGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                                 >  2:210020/1‑101 (MQ=255)


                                                      cGGCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                                 <  1:2323084/101‑1 (MQ=255)


                                                       ggCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                                <  2:3699493/101‑1 (MQ=255)


                                                       ggCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                                >  1:2118725/1‑101 (MQ=255)


                                                       ggCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                                <  2:4929724/101‑1 (MQ=255)


                                                        gCATGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgag                                               <  1:2930745/101‑1 (MQ=255)


                                                          aTGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                             <  1:3298524/101‑1 (MQ=255)


                                                          aTGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                             >  1:1025174/1‑101 (MQ=255)


                                                          aTGTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGACCCGagag                                             >  2:4040814/1‑101 (MQ=255)


                                                            gTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAggg                                           >  1:384542/1‑101 (MQ=255)


                                                            gTCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAggg                                           >  1:4279133/1‑101 (MQ=255)


                                                             tCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                          >  1:11649/1‑101 (MQ=255)


                                                             tCGATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                          >  1:923273/1‑101 (MQ=255)


                                                               gATCGCGGCGGTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                        <  2:836839/101‑1 (MQ=255)


                                                                aTCGCGGCGGTCGACGCGCGCGAGC.TTGGCCCCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGt                                       <  1:3431830/101‑1 (MQ=255)


                                                                  cgcgGCGGTCGACGCGCGCGAGC.TTGGCCCCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                                     <  1:3805057/101‑1 (MQ=255)


                                                                    cggcggTCGACGCGGGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.g                                  <  1:4354579/101‑1 (MQ=255)


                                                                    cggcggTCGACGCGCGCGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.g                                  <  1:4234491/101‑1 (MQ=255)


                                                                      gcggTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGa                                >  1:3127168/1‑101 (MQ=255)


                                                                        ggTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAc                              >  2:1372094/1‑101 (MQ=255)


                                                                         gTCGACGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGGCGCACCGGAACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAc                              >  2:3532641/1‑101 (MQ=255)


                                                                         gTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCCCCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAcg                             >  1:1984661/1‑101 (MQ=255)


                                                                         gTCGACGCGCGCGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAcg                             >  1:4955337/1‑101 (MQ=255)


                                                                           cgacncgcgcGAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAcggc                           <  1:4035011/95‑1 (MQ=255)


                                                                            gacgcgcgcGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAAcggc.g                         >  2:1886694/6‑101 (MQ=255)


                                                                               gcgcgcGAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGc                      >  2:4648017/3‑101 (MQ=255)


                                                                                 gcgcGAGC.TTGGCACCT.C.ACCCCCCCCCCGGCGCCCCGGCACACCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGGGGGCAGTCAC.GAAACGGC.GGGcc                     >  1:4889147/1‑101 (MQ=255)


                                                                                  cgcgAGC.TTGGCCCCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCg                   <  2:2306275/101‑1 (MQ=255)


                                                                                  cgcgAGC.TTGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCg                   <  1:1183812/101‑1 (MQ=255)


                                                                                  cgcgAGC.TTGCCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCg                   <  1:1411118/101‑1 (MQ=255)


                                                                                    cgAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTGAA.GGAAcggc.ggcggccac                 >  2:3940695/1‑94 (MQ=255)


                                                                                    cgAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGGCCGaa                 >  1:3853896/1‑101 (MQ=255)


                                                                                    cgAGC.TTGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGaa                 >  1:4136448/1‑101 (MQ=255)


                                                                                      gagcttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCCCAGCCCGAGAGCCCGGCGGCCACAGCCAGCCCCGAGGGTGCAGGCAAGGGAAGGGG.GGGcccacg                 >  2:1659636/5‑98 (MQ=255)


                                                                                       agcttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGCAgc               >  2:2133296/4‑101 (MQ=255)


                                                                                         cttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGccgcgagtgg             >  2:2687072/2‑93 (MQ=255)


                                                                                         cttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAgcgc             <  1:3380072/100‑1 (MQ=255)


                                                                                         cttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAacgc             >  2:3378317/2‑101 (MQ=255)


                                                                                          ttGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGGCCGAAgcggc            >  2:2240710/1‑99 (MQ=255)


                                                                                          ttGGCACCT.C.AC.CCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGGCAA.GGAACGGCGGGGCCCGagggca             >  1:2628388/1‑96 (MQ=255)


                                                                                           tGGCACCTCC.C..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCaa           <  2:1148824/101‑1 (MQ=255)


                                                                                           tGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAgcgcca           >  2:4344570/1‑99 (MQ=255)


                                                                                           tGGCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCaa           <  2:1423710/101‑1 (MQ=255)


                                                                                            ggCACCT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCCAAGCGCAAg          >  1:4885044/1‑101 (MQ=255)


                                                                                             gCACCT.C.CC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCAAgg         <  2:3608712/101‑1 (MQ=255)


                                                                                                ccT.C.AC.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGCGGGGCCCGAA.CGCAAAgcgc      >  2:4691851/1‑101 (MQ=255)


                                                                                                    c.A..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCAAGGCGCCCgg  >  2:2110884/1‑101 (MQ=255)


CAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACG.GCGCGAGCTTTGGCACCT.C.A..CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAA.GGAACGGC.GGGCCCGAAGCGCAAGGCGCCCGG  >  NC_014034/1745355‑1745553

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 21 ≤


ATCG

< 31 ≤


ATCG

< 40 ≤


ATCG