BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	89.3%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	89.3%	68.6 ‑0.2 ‑0.9	215	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (90/102): ref base (10/13): total (100/115)
Fisher's exact test strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.27e-01

Only 100 of 228 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


tGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.c                                                                                                  <  2:2698683/101‑1 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.g                                                                                                >  2:1698814/1‑101 (MQ=255)


  aTCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.gg                                                                                               >  1:278981/1‑100 (MQ=255)


  aTCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.gg                                                                                               <  2:3450705/101‑2 (MQ=255)


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcc                                                                                            <  1:3691325/101‑1 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gccca                                                                                          >  1:2473669/1‑99 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gccca                                                                                          <  2:1286208/101‑3 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaac                                                                                        >  1:1379456/1‑97 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaac                                                                                        >  1:1263323/1‑97 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaac                                                                                        <  2:4611780/101‑5 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaac                                                                                        <  1:4212856/101‑5 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaaccctc                                                                                    <  1:1745913/101‑9 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.gcccaaccctc                                                                                    <  1:2097028/101‑9 (MQ=255)


             gcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCg                                                                                    <  1:3234804/101‑1 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCgg                                                                                   >  1:891698/1‑101 (MQ=255)


                 cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGc                                                                                <  2:3720718/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCt                                                                               <  2:4222475/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCt                                                                               <  2:2473495/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGc                                                                             <  1:4009027/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGt                                                                          >  1:329772/1‑101 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                         >  2:3307512/1‑101 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                         <  1:4426304/101‑1 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTa                                                                         <  2:1582993/101‑1 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcgc                                                                     <  2:2237847/101‑5 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTagcgc                                                                     >  1:308470/1‑97 (MQ=255)


                              gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTc                                                                   >  2:2610776/1‑101 (MQ=255)


                               tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  <  2:652102/101‑1 (MQ=255)


                               tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  >  2:4149575/1‑101 (MQ=255)


                               gccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  <  1:1000489/100‑1 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCgg                                                                 <  1:3263826/101‑1 (MQ=255)


                                 cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  2:2957573/1‑101 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               <  2:4286157/101‑1 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               >  1:127913/1‑101 (MQ=255)


                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             >  1:2880278/1‑101 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            >  2:1105185/1‑101 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCAGGTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  2:2654663/101‑1 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           >  1:705431/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATTACCTTGCGGGTGGTGAACT.CGG.CCCAACCTTCGGGGATGCGGTAGCGCTCGGGCGtatt                                                          >  2:833165/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  2:1751501/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  2:1982592/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:979383/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:1560129/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:2790958/101‑1 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       <  1:3876489/101‑1 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       <  1:1968355/101‑1 (MQ=255)


                                            gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                     <  2:3356562/101‑1 (MQ=255)


                                            gagggggaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGTGTTTTCgggt                                                     >  1:2498736/1‑99 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  1:2377867/1‑101 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  2:1813348/1‑101 (MQ=255)


                                               ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                  >  2:505754/1‑101 (MQ=255)


                                                gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 >  1:1448359/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  2:4591143/1‑101 (MQ=255)


                                                   aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              <  1:2957801/101‑1 (MQ=255)


                                                    tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             <  2:4688081/101‑1 (MQ=255)


                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            >  1:1352613/1‑101 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:2219586/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:2706783/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  2:2757845/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:499114/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           >  1:3896134/1‑101 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:103039/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          <  2:3674145/101‑1 (MQ=255)


                                                        tctggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  2:754933/98‑1 (MQ=255)


                                                         gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                        <  2:1641021/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     <  2:4395037/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGGCGaa                                     >  2:1541023/1‑101 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  2:4198177/101‑1 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    >  2:4911078/1‑101 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  2:2498562/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   >  1:4692071/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               <  2:1506000/101‑1 (MQ=255)


                                                                  ttGACCGTGACGATCAACTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCTGATCAAGTTCCTCGACGAACAGGTc                               <  1:833267/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             >  1:3562746/1‑101 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACG.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          <  1:2453773/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         >  1:3422434/1‑101 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:3371045/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:2537965/101‑1 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        >  1:514493/1‑101 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        <  1:1698944/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:1043209/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       >  1:4901669/1‑101 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  1:1484395/101‑1 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     >  2:2760901/1‑101 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  1:3910217/1‑101 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:3881861/1‑101 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:2900795/1‑101 (MQ=255)


                                                                              aTCACCTTGCGGGTGGTGAACG.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGc                   <  2:5012769/101‑1 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 <  2:1348687/101‑1 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  2:2419746/1‑101 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 <  1:4941749/101‑1 (MQ=255)


                                                                                 aCCTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                <  1:1114256/101‑1 (MQ=255)


                                                                                   cTTGCGGGTGGTGAACTAG.G.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGAGAAgg              >  1:3596204/1‑101 (MQ=255)


                                                                                   cTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg              >  1:3018969/1‑101 (MQ=255)


                                                                                   cTTGCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg              <  1:744126/101‑1 (MQ=255)


                                                                                      gCGGGTGGTGAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  1:1637999/101‑1 (MQ=255)


                                                                                           tggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      >  2:1782651/1‑101 (MQ=255)


                                                                                            ggtgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     <  2:3197622/101‑1 (MQ=255)


                                                                                              tgAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg   <  2:3334054/101‑1 (MQ=255)


                                                                                              tgAACT.C.GTCCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg   <  1:483249/101‑1 (MQ=255)


                                                                                               gAACT.CGG.CCCAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  2:2947884/101‑1 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.G.CCC.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG