Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,084 +C 100% coding (26/879 nt) potH1 ← polyamine ABC transporter permease PotH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,0841.→C100.0% 872.2 231coding (26/879 nt)potH1polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand):  new base (108/123):  ref base (0/0):  total (108/123)

Only 100 of 265 total aligned reads displayed.

GAATAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAA  >  NC_014034/2046985‑2047174
                                                                                                     |                                                                                                
gAATAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAgcgc.g.c                                                                                                 >  1:4235550/1‑101 (MQ=255)
gAATAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAgcgc.g.c                                                                                                 >  1:1246245/1‑101 (MQ=255)
gAATAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAgcgc.g.c                                                                                                 >  2:438795/1‑101 (MQ=255)
 aaTAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.cc                                                                                                >  1:604067/1‑101 (MQ=255)
 aaTAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.cc                                                                                                >  1:2689938/1‑101 (MQ=255)
  aTAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.ccc                                                                                               <  1:2429587/101‑2 (MQ=255)
   tAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.ccca                                                                                              >  1:3199492/1‑99 (MQ=255)
    aGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.cccac                                                                                             >  2:2921614/1‑98 (MQ=255)
    aGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.cccac                                                                                             >  2:2526625/1‑98 (MQ=255)
     ggCGGCGATGGTCACCAGCGGGCCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCAGGACCCAGCCGCTGGCCGCCTCCGAGCGC.G.cccacc                                                                                            <  2:3116921/101‑5 (MQ=255)
       cgacgaGGGTCCCCGGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACcg.c                                                                                          <  2:961593/101‑1 (MQ=255)
        gccgaTGGTCCCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACcg.cc                                                                                         <  1:3084319/99‑2 (MQ=255)
          cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgg                                                                                       >  2:2620481/1‑101 (MQ=255)
           gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccggg                                                                                      <  1:1998339/101‑2 (MQ=255)
            aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCAGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggt                                                                                     >  1:4352384/1‑99 (MQ=255)
             tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggtt                                                                                    <  1:452581/101‑4 (MQ=255)
              ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCAGAGATCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttg                                                                                   <  2:4781307/101‑5 (MQ=255)
               gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttgg                                                                                  <  2:1385195/101‑6 (MQ=255)
               gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttgg                                                                                  >  2:36218/1‑96 (MQ=255)
                tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttgga                                                                                 <  2:3893008/101‑7 (MQ=255)
                  aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttggaga                                                                               >  1:966643/1‑93 (MQ=255)
                  aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCG.Ccgggttggaga                                                                               >  2:2316904/1‑93 (MQ=255)
                   ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGAt                                                                               <  1:1441101/101‑1 (MQ=255)
                     aGCGGGTCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGc                                                                             <  2:2223036/101‑1 (MQ=255)
                     aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGc                                                                             >  1:2173236/1‑101 (MQ=255)
                     aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGc                                                                             <  1:4682325/101‑1 (MQ=255)
                     aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGc                                                                             >  1:3491823/1‑101 (MQ=255)
                        ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGa                                                                          >  1:4472460/1‑101 (MQ=255)
                         gggCCGCCAGCAACAGAACTGCATGGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGAc                                                                         <  2:4916149/101‑1 (MQ=255)
                         gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGAc                                                                         >  1:2985709/1‑101 (MQ=255)
                         gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGAc                                                                         <  2:2822355/101‑1 (MQ=255)
                         gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGAc                                                                         <  2:4627503/101‑1 (MQ=255)
                          ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACa                                                                        >  1:1717113/1‑101 (MQ=255)
                            ccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATa                                                                      >  1:4968545/1‑101 (MQ=255)
                            ccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATa                                                                      <  2:3016718/101‑1 (MQ=255)
                               ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                   >  2:176170/1‑101 (MQ=255)
                                cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                  >  2:1698806/1‑101 (MQ=255)
                                 aGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTa                                                                 >  2:2932788/1‑101 (MQ=255)
                                  gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                <  1:4579655/101‑1 (MQ=255)
                                  gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTAt                                                                >  1:211983/1‑101 (MQ=255)
                                   cAACAGAACTTCATAGATCGGTGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                               <  2:4833725/101‑1 (MQ=255)
                                   cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                               >  2:4101519/1‑101 (MQ=255)
                                   cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTAtt                                                               >  1:2943204/1‑101 (MQ=255)
                                    aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                              >  1:3301064/1‑101 (MQ=255)
                                    aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                              <  1:4376115/101‑1 (MQ=255)
                                      aaGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct.c                                                           <  1:2575705/100‑1 (MQ=255)
                                       aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                          >  2:2857342/1‑101 (MQ=255)
                                       aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.cc                                                          <  2:1510488/101‑1 (MQ=255)
                                         atcTGCATAGATCAGCGTTGGGCTCACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                        <  2:2985481/99‑3 (MQ=255)
                                         aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                        >  2:3385060/1‑99 (MQ=255)
                                          aCTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                       <  1:1138280/101‑4 (MQ=255)
                                          aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                       <  2:1130835/101‑4 (MQ=255)
                                          aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctc                                                       <  2:1421634/101‑4 (MQ=255)
                                           cTGCAAAGATCAGGGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                      <  1:823688/101‑5 (MQ=255)
                                             gaaTAGATCAGCGTTGGGTTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgc                                                    <  2:1481851/99‑1 (MQ=255)
                                             gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgc                                                    <  2:513339/101‑1 (MQ=255)
                                              cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgcg                                                   >  2:63186/1‑101 (MQ=255)
                                              cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgcg                                                   <  2:4472141/101‑1 (MQ=255)
                                                    tCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgcgcgtctc                                             <  2:1237512/101‑6 (MQ=255)
                                                    tCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAgcgcgtctc                                             <  2:4326573/101‑6 (MQ=255)
                                                     cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCa                                            >  1:1497613/1‑101 (MQ=255)
                                                      aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.g                                          >  1:1663525/1‑101 (MQ=255)
                                                      aGCGTTGGGCCGACCATGAACCAGCCGCTGGTCGCCTCCGAGCGCAA.CAACCGCCGGGATCGAGATGCCGGCATGCTCCATTCA.CCGTCAgcgcgcctccg                                           >  2:1650351/1‑94 (MQ=255)
                                                       gCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.gg                                         <  2:3715667/101‑2 (MQ=255)
                                                       gCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.gg                                         >  2:3173573/1‑100 (MQ=255)
                                                          ttGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.ggcgc                                      >  2:3536387/1‑97 (MQ=255)
                                                          ttGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.ggcgc                                      >  1:1832931/1‑97 (MQ=255)
                                                           tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.ggcgcg                                     >  1:3701741/1‑96 (MQ=255)
                                                           tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.ggcgcg                                     >  2:3371063/1‑96 (MQ=255)
                                                           tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCA.ggcacg                                     >  1:4817164/1‑96 (MQ=255)
                                                              gCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGttt                                   <  1:1235892/101‑1 (MQ=255)
                                                              gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGttt                                   >  2:1555505/1‑101 (MQ=255)
                                                               cTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                  <  1:2238113/101‑1 (MQ=255)
                                                               cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                  <  1:1413966/101‑1 (MQ=255)
                                                               cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                  >  2:3801008/1‑101 (MQ=255)
                                                               cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                  >  2:1815788/1‑101 (MQ=255)
                                                               cTGACCATAACCCAGCCGCTGGCAGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGTCATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTg                                  <  2:4976111/101‑1 (MQ=255)
                                                                tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTgg                                 <  1:2052018/101‑1 (MQ=255)
                                                                   ccATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGcc                              >  1:694334/1‑101 (MQ=255)
                                                                    cATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCa                             >  1:1910762/1‑101 (MQ=255)
                                                                    cATGAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTAGGCCa                             >  2:2487/1‑101 (MQ=255)
                                                                       gAACCAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAAt                          >  1:4865084/1‑101 (MQ=255)
                                                                           cAGCCGCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGc                      >  1:1028874/1‑101 (MQ=255)
                                                                                gCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                 <  2:2149952/101‑1 (MQ=255)
                                                                                gCTGGCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                 <  2:603988/101‑1 (MQ=255)
                                                                                   ggCCGCCTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt              <  1:4114890/101‑1 (MQ=255)
                                                                                    gccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt             <  2:762485/101‑1 (MQ=255)
                                                                                     ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc            <  2:3664440/101‑1 (MQ=255)
                                                                                     ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc            <  2:5026180/101‑1 (MQ=255)
                                                                                     ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc            <  1:176186/101‑1 (MQ=255)
                                                                                     ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc            <  1:4029327/101‑1 (MQ=255)
                                                                                     ccgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc            <  1:2914318/101‑1 (MQ=255)
                                                                                      cgccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg           <  2:4255478/101‑1 (MQ=255)
                                                                                        ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc         <  1:889223/101‑1 (MQ=255)
                                                                                        ccTCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc         <  2:3257914/101‑1 (MQ=255)
                                                                                          tCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag       <  2:3199241/101‑1 (MQ=255)
                                                                                          tCCGAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag       <  2:2963621/101‑1 (MQ=255)
                                                                                             gAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg    >  2:4698439/1‑101 (MQ=255)
                                                                                             gAGCGC.GCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg    <  2:4056995/101‑1 (MQ=255)
                                                                                               gcgc.gcCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCC.TCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa  <  2:126903/101‑1 (MQ=255)
                                                                                                     |                                                                                                
GAATAGGCGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGC.G.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CC.TCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAA  >  NC_014034/2046985‑2047174

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG