Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,070,633	+G	100%	intergenic (+48/+124)	dcd → / ← guaB	deoxycytidine triphosphate deaminase/inosine‑5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,070,633	1	.→G	100.0%	107.3	42	intergenic (+48/+124)	dcd/guaB	deoxycytidine triphosphate deaminase/inosine‑5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (1/41):  ref base (0/0):  total (1/41)

CGAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTCCCC  >  NC_014034/2070533‑2070728                                                                                                          |                                                                                                 cGAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgcc                                                                                                   >  2:1823708/1‑101 (MQ=255) cGAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgcc                                                                                                   >  1:828978/1‑101 (MQ=255)  gAACTATCAGGGCCAGGGTCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgccc                                                                                                  >  1:3867910/1‑100 (MQ=255)  gAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgccc                                                                                                  >  1:4546674/1‑100 (MQ=255)   aaCTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgcccg                                                                                                 >  1:632898/1‑99 (MQ=255)   aaCTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgcccg                                                                                                 >  1:4127290/1‑99 (MQ=255)    aCTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..cgg                                                                                              >  1:2138317/1‑101 (MQ=255)      tATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACccgcccgtgg                                                                                              >  2:4259069/1‑96 (MQ=255)      tATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..ggggg                                                                                            >  1:2535314/1‑101 (MQ=255)       aTCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..gggggg                                                                                           >  1:4081838/1‑100 (MQ=255)          aGGGCCGGGGCCGGAAGCTCCCCAAGCTTTCCCGCGGGGGGG.GGGGGGCGCGCGGATGGTTTCCG.CCGCGGCTTT.CCC.TGCGGCGCCCCGCC..ggggggcgc                                                                                        <  2:2974746/101‑5 (MQ=255)          aGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..ggggggggg                                                                                        >  2:1584820/1‑97 (MQ=255)              cggggggGTGAACCCCGCCAAGCTTTCCGGGGCGGGGG.GAGGGGCGCGCGGATGGTTTCCG.CCGCGGCTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGcc                                                                                     <  2:360295/98‑1 (MQ=255)                 ggggCTGAAGCCCGCCAAGCTTTCCCGGGGGGGGG.GGGGGGCGCGGGGATGGTTTCCC.CCGGGGCTTT.CCC.GGGGGCGCCCCGCCG.GGGGGCGCTGccccc                                                                                  <  2:3808826/101‑1 (MQ=255)                  gggCCGAAGCCCGCCAAGCTTTCCCGCGGGGGGG.GGGGGGCGCGGGGATGGTTTTCG.CGGCGGCTTT.GCC.GGCGGCCCCCCGCCG.GGGGGCGCTGcccccc                                                                                 <  2:699122/101‑1 (MQ=255)                  ggacagAAGCTCGCCACGTGTTCCCGCGGGGGGT.GGGGGGCGCGGGGTTGGTTTCCC.CGGCGGTTTT.GCC.GGGGGCGCCCCGCCG.GGGGGCGCTGcccccc                                                                                 <  2:2140073/96‑1 (MQ=255)                    gCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCCG.GGGGGGGGTGcccccccc                                                                               >  2:645992/1‑99 (MQ=38)                    gCTGAAGCGCGCCACGCTTCTCGGGGGGGGGG.GGGGGGCGCGGGGATGGTTTCCG.CCGGGGCTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCgg                                                                               <  2:4918042/101‑1 (MQ=255)                        aggcccGCCAAGCATGGCCGCGGGGGGG.GGGGGGCGCGCGGAGGGTTTCCG.CCGCGGTTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGAccc                                                                           <  2:4532364/96‑1 (MQ=19)                        acgcccGCCAAGCTTTCCCGGGGGGGGG.GGGGGGCGCGCGTGTGGTTTCCC.CCGCGGCTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGAccc                                                                           <  1:2971012/96‑1 (MQ=16)                        aaGCCCGACAAGCCTGTCCGCGCGGGGG.GAGGGGCGCCAGGTGCGTTTTCG.CGTCTTCTGC.GCC.CGCGGCAACCCGCCG.GGGGGCGCTGCCCCCCGGAccc                                                                           <  2:734722/101‑1 (MQ=9)                          gcccGCCAATCTTCTCCGCGGGGGGT.GGGGGGCGGGCGAATGGTTTCCG.CCGCGGCTTG.GCC.TGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGAccccc                                                                         <  2:4081535/98‑1 (MQ=16)                           ccccccAAGC.TTTCCGGGGGGGGTGGGGGGGCGCGCGGTTGTTTTCCG.CCGCGGCTTT.GCT.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGAcccccc                                                                        <  2:3313293/97‑1 (MQ=12)                           cTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCCCGGGGGGGGCGGccccccccgccccc                                                                         >  2:573205/1‑93 (MQ=255)                              ggcactcATTCCCGGGGGGGGG.GGGGGGCGCGGGTATGGTTTTGG.TTCCTGTTCT.CCC.GGCGCCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCggg                                                                     <  1:3386877/95‑1 (MQ=2)                                         ccggggggggG.GGGGGGCGGGGGGGTGTTTTCCG.CCGCGGCTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGc                                                          <  1:2426669/95‑1 (MQ=16)                                         ccggcgggggG.GGGGGGCGCGCGGAGGTGTTCCG.CGGCGTTTTC.GCC.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGc                                                          <  1:726039/95‑1 (MQ=9)                                         ccGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGAGACCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGc                                                          <  1:2352999/101‑1 (MQ=255)                                          ggcgggGGGG.GGGGGGCGCGGGGATGGTTTTCG.CCGCGGCTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCa                                                         <  2:3867618/100‑1 (MQ=21)                                          cgcgggGGGG.GGGGGGGGCGGGGTTGGTTTCCC.CCTGGTCTTC.GCG.CGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCa                                                         <  2:2851501/101‑1 (MQ=9)                                           cgggggggG.GGGGGGCGCGCGGTTGTTTTCCC.CCGCGGTTTT.CCC.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCaa                                                        <  1:3916445/97‑1 (MQ=12)                                             ggggggG.GGGGGGGGGGGGGGTGGTTTCCG.CCGCGGTTTT.GCC.GGGGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGa                                                      <  1:3344234/99‑1 (MQ=9)                                              gggggG.GGGGGGCGGGGGGTTGGTTTGCG.CCGGGGTTTG.GCG.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGAt                                                     <  1:989950/100‑1 (MQ=9)                                               ggggG.GGGGGGGGGGGGGGTGGTTTCCG.CGGGGGCTTG.CCG.TGCGGGGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATg                                                    <  1:3677775/101‑1 (MQ=9)                                               ggggG.GGGGGGGGCGGGGATGTTTTCCG.CCGCGCCTGG.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATg                                                    <  2:4781670/101‑1 (MQ=12)                                               ggggG.GGGGGCCGCGGGGTGGGTTTCCG.CCGCGGTTTC.GCC.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATg                                                    <  2:2671678/101‑1 (MQ=9)                                                 ggggggggggCGCGGGGATGGTTTCCG.CCGCGGTTTT.GCC.GGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGa                                                   <  2:828876/95‑1 (MQ=18)                                                    gggggggCGCGGGGAGTGTTTCCC.CCGCTGTTTG.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAgg                                                <  2:3958658/98‑1 (MQ=12)                                                      gggggCGGGGGGTTGGTTTCCG.CCGCGTCTTG.GCG.GGCGCCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAgggg                                              <  1:2813533/100‑1 (MQ=12)                                                      gggggCGCGCGGATGGTTTGCC.CCGCGCCTTC.CCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAgggg                                              <  1:3284550/100‑1 (MQ=18)                                                       ggggGGCGGGGGTGTTTTCCG.CCGCGTTTTT.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGa                                             <  1:2251856/101‑1 (MQ=17)                                                        gggCGGGGGGGTGGTTTCCG..CGCGGTTTTCGCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGaa                                            <  2:4870348/101‑1 (MQ=12)                                                        gggCGCGCGG.TTGTTTTCGCCCGGGGTTTG.GCC.GGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGaa                                            <  1:4151116/101‑1 (MQ=12)                                                                gggtggttcccg.ccgcCGCTTG.CCC.CCCCGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGtt                                    <  1:99823/91‑1 (MQ=17)                                                                gggtgggtTCCG.GCGCGGCTTG.GCC.CGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGtt                                    <  1:3782933/98‑1 (MQ=18)                                                                 gggtgttgccg.ccgcGTCTTG.CCC.GGCGCCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGttt                                   <  2:1266499/92‑1 (MQ=17)                                                                 gatgttttgcg.ccgcGG.TTT.GCCGGGCGGCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGttt                                   <  2:2159599/92‑1 (MQ=17)                                                                         tgcgcgggggCTTC.CCC.TGCGCCCCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTa                            <  2:3155762/93‑1 (MQ=18)                                                                                   ggtttgcccggcggccCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGaaa                    <  2:318500/91‑1 (MQ=21)                                                                                    ttt.GCC.TGCGGCGCCCCGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAg                 <  2:3400312/101‑1 (MQ=255)                                                                                              cgcccccccGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCTgggg         <  1:4380848/95‑1 (MQ=25)                                                                                               gcccccccGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGt        <  2:1246829/96‑1 (MQ=255)                                                                                                gccccccGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGtt       <  1:573284/97‑1 (MQ=255)                                                                                                gccccccGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGtt       <  2:2412601/97‑1 (MQ=255)                                                                                                  cccccGCCG.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTc     <  1:4399923/99‑1 (MQ=255)                                                                                                     ccgccg.GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTcccc  <  1:5368/101‑1 (MQ=255)                                                                                                          |                                                                                                 CGAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGT.GAGGGGCGCGCGGATGGATTTCG.CCGCGGCTTT.GCC.TGCGGCGACCCGCC..GGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTCCCC  >  NC_014034/2070533‑2070728 Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG