Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,165,872 +C 100% intergenic (‑177/‑207) RCAP_rcc02007 ← / → tig phage integrase/trigger factor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,165,8721.→C100.0% 646.7 169intergenic (‑177/‑207)RCAP_rcc02007/tigphage integrase/trigger factor
Reads supporting (aligned to +/- strand):  new base (89/80):  ref base (0/0):  total (89/80)

Only 100 of 186 total aligned reads displayed.

GGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.C.TC.TG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165777‑2165955
                                                                                                    |                                                                                   
ggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTG.ccc                                                                                  >  1:3930035/1‑101 (MQ=255)
ggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTG.ccc                                                                                  >  1:2131232/1‑101 (MQ=255)
ggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCAGCACCATCTGACCTTTCC.TC.TGCCCTG.ccc                                                                                  >  2:312529/1‑101 (MQ=255)
 gcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTG.cccc                                                                                 >  1:785332/1‑100 (MQ=255)
    ttGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTG.ccccgcg                                                                              >  2:3356257/1‑97 (MQ=255)
     tGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTG.ccccgcgc                                                                             >  2:4341020/1‑96 (MQ=255)
      gagaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCt                                                                             >  2:2720338/1‑101 (MQ=255)
      gagaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TG.CCCT.CCCccgccct                                                                           >  1:3778165/1‑98 (MQ=255)
        gaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGc                                                                           >  2:1820312/1‑101 (MQ=255)
        gaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGc                                                                           >  1:70176/1‑101 (MQ=255)
        gaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGc                                                                           <  1:3294672/101‑1 (MQ=255)
        gaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGc                                                                           >  1:768231/1‑101 (MQ=255)
        gaTTTCCCGGGGCGCAGAGGCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGc                                                                           >  1:4740867/1‑101 (MQ=255)
             cccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGtct                                                                      >  2:1275812/1‑101 (MQ=255)
             cccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGtct                                                                      <  2:2186592/101‑1 (MQ=255)
             cccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGtct                                                                      <  1:1634775/101‑1 (MQ=255)
              ccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGtctc                                                                     <  1:3521311/101‑1 (MQ=255)
               cGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCg                                                                    <  1:595125/101‑1 (MQ=255)
               cGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCg                                                                    >  2:3949779/1‑101 (MQ=255)
                ggggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCgg                                                                   <  1:484121/101‑1 (MQ=255)
                ggggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCgg                                                                   <  1:4180723/101‑1 (MQ=255)
                 gggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCggg                                                                  >  1:1668564/1‑101 (MQ=255)
                  ggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGGGCAGACCACCAGCACCATCTGACCTTT.CATCGAC.CCTGCCCCGCGCTGCCGTCTCgggg                                                                 >  1:1976482/1‑101 (MQ=255)
                   gcgcAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGc                                                                <  2:1432536/101‑1 (MQ=255)
                    cgcAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCg                                                               >  1:3101543/1‑101 (MQ=255)
                     gcAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgt                                                              <  2:1976338/101‑1 (MQ=255)
                      cAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtc                                                             >  2:2065816/1‑101 (MQ=255)
                       agagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtct                                                            <  1:443410/101‑1 (MQ=255)
                        gagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctg                                                           <  2:876055/101‑1 (MQ=255)
                         agTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgt                                                          <  2:2094899/101‑1 (MQ=255)
                          gTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGTCgtctgtc                                                         <  2:1668435/101‑1 (MQ=255)
                          gTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtc                                                         <  1:2738507/101‑1 (MQ=255)
                          gTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtc                                                         >  2:3865339/1‑101 (MQ=255)
                           tCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtct                                                        <  1:4454717/101‑1 (MQ=255)
                            cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGtctgtctg                                                       >  2:302378/1‑101 (MQ=255)
                             aaCCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTgg                                                      >  2:2147491/1‑101 (MQ=255)
                              aCCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTggg                                                     <  1:3079231/101‑1 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    <  1:4276165/101‑1 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    >  2:1518148/1‑101 (MQ=255)
                                cTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCg                                                   <  2:3901590/101‑1 (MQ=255)
                                cTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCg                                                   >  1:586026/1‑101 (MQ=255)
                                cTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCg                                                   <  1:1943001/101‑1 (MQ=255)
                                 tGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGa                                                  >  1:3379368/1‑101 (MQ=255)
                                  gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 >  1:1594444/1‑101 (MQ=255)
                                   agaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAAt                                                >  2:2812555/1‑101 (MQ=255)
                                    gaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATc                                               >  1:1904536/1‑101 (MQ=255)
                                     aCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCt                                              <  2:1003987/101‑1 (MQ=255)
                                     aCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCt                                              >  1:4430524/1‑101 (MQ=255)
                                      cGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCtt                                             <  2:4261723/101‑1 (MQ=255)
                                        gCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCtttt                                           >  1:1665816/1‑101 (MQ=255)
                                        gCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATAtttt                                           <  1:3865631/101‑1 (MQ=255)
                                         cATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTc                                          <  1:2828173/101‑1 (MQ=255)
                                          aTGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCg                                         >  2:144911/1‑101 (MQ=255)
                                            gCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGAt                                       <  1:2481015/101‑1 (MQ=255)
                                             cAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATc                                      >  1:4079262/1‑101 (MQ=255)
                                              aaCACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCt                                     <  2:4955013/101‑1 (MQ=255)
                                              aaCACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCt                                     <  2:585947/101‑1 (MQ=255)
                                               acacGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCtt                                    <  2:431414/101‑1 (MQ=255)
                                                  cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 >  1:81279/1‑101 (MQ=255)
                                                  cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 <  1:2999603/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTg                                <  2:2586007/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTg                                <  1:4009901/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTg                                <  2:4510638/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTg                                <  2:3210412/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATATTTCTg                                >  2:1423185/1‑101 (MQ=255)
                                                    gCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGCCTGTCTGGGCGAATCTTTTCGATCTTTCTGc                               >  2:3482084/1‑101 (MQ=255)
                                                     cACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGcc                              <  1:1052722/101‑1 (MQ=255)
                                                     cACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGcc                              >  2:678320/1‑101 (MQ=255)
                                                       cTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCcgc                            >  2:220826/1‑101 (MQ=255)
                                                       cTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCcgc                            >  1:1211905/1‑101 (MQ=255)
                                                       cTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCcgc                            <  2:742876/101‑1 (MQ=255)
                                                         ggTGCAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCgcgc                          >  2:3654439/1‑101 (MQ=255)
                                                             cAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCggg                      <  1:4888364/101‑1 (MQ=255)
                                                             cAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCggg                      <  1:201251/101‑1 (MQ=255)
                                                             cAGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCggg                      >  1:1304600/1‑101 (MQ=255)
                                                              aGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGa                     >  2:4885582/1‑101 (MQ=255)
                                                              aGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGa                     <  2:2946904/101‑1 (MQ=255)
                                                              aGACCACCCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGa                     >  2:1854113/1‑101 (MQ=255)
                                                                accaccCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   <  2:3939880/101‑1 (MQ=255)
                                                                accaccCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   >  1:3824011/1‑101 (MQ=255)
                                                                 ccaccCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCg                  <  1:568411/101‑1 (MQ=255)
                                                                  caccCGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGa                 <  2:2326668/101‑1 (MQ=255)
                                                                    cccGCACCATCTGGCCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaa               <  1:4341337/101‑1 (MQ=255)
                                                                    cccGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaa               <  1:1465796/101‑1 (MQ=255)
                                                                     ccGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaaa              <  2:3593081/101‑1 (MQ=255)
                                                                     ccGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaaa              >  1:2351279/1‑101 (MQ=255)
                                                                      cGCACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAAc             >  2:2453576/1‑101 (MQ=255)
                                                                        cACCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACgg           <  2:744496/101‑1 (MQ=255)
                                                                         aCCATCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGc          >  2:5044838/1‑101 (MQ=255)
                                                                            aTCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       <  2:969423/101‑1 (MQ=255)
                                                                            aTCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       >  1:3152172/1‑101 (MQ=255)
                                                                            aTCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       >  1:2070177/1‑101 (MQ=255)
                                                                             tCTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCgg      >  2:3439474/1‑101 (MQ=255)
                                                                              cTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGa     >  2:3802843/1‑101 (MQ=255)
                                                                              cTGACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGa     >  2:898133/1‑101 (MQ=255)
                                                                                gACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaa   >  2:4719194/1‑101 (MQ=255)
                                                                                gACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaa   >  2:409604/1‑101 (MQ=255)
                                                                                gACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCCGaaa   >  2:4768119/1‑101 (MQ=255)
                                                                                gACCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCCGaaa   >  1:1015961/1‑101 (MQ=255)
                                                                                 aCCTTTCC.TC.TGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaaa  >  2:3411750/1‑101 (MQ=255)
                                                                                                    |                                                                                   
GGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.C.TC.TG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165777‑2165955

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG