Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,206,353 +G 2.9% coding (909/3258 nt) cobN ← cobaltochelatase subunit CobN

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,206,3531.→G 2.9% 7.8 ‑4.5 ‑0.0173coding (909/3258 nt)cobNcobaltochelatase subunit CobN
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (66/102):  total (71/102)
Fisher's exact test strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.05e-01

Only 100 of 201 total aligned reads displayed.

CCAGATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGCGTCGTGC  >  NC_014034/2206253‑2206453
                                                                                                     |                                                                                                     
ccAGATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCa                                                                                                        >  1:1769712/1‑101 (MQ=255)
  aGATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGCTCGGCA.gg                                                                                                     >  2:2668896/1‑101 (MQ=255)
  aGATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.gg                                                                                                     <  1:3991230/101‑1 (MQ=255)
   gATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.ggg                                                                                                    >  1:4260873/1‑100 (MQ=255)
   gATCGGGGTAGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.ggg                                                                                                    >  2:4112774/1‑100 (MQ=255)
    ctCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCg                                                                                                   <  1:1499015/100‑1 (MQ=255)
    aTCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.gggg                                                                                                   >  2:2131196/1‑99 (MQ=255)
    aTCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCg                                                                                                   >  1:3149839/1‑101 (MQ=255)
       ggggTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.gaa                                                                                               >  1:1616814/1‑101 (MQ=255)
       ggggTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.aaa                                                                                               <  1:619408/101‑1 (MQ=255)
       ggggTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.aaa                                                                                               >  2:3590184/1‑101 (MQ=255)
         ggTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.aaacg                                                                                             >  1:1242474/1‑99 (MQ=255)
         ggTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.aaaag                                                                                             >  1:1046763/1‑99 (MQ=255)
           tCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.GAAGGcc                                                                                           >  1:2523359/1‑101 (MQ=255)
            cgcgcgGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.gggggaaaggac                                                                                           >  2:2021350/1‑91 (MQ=255)
            cgcgcgGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.aaaaggcg                                                                                          >  1:2750884/1‑96 (MQ=255)
             gcgcgGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCcgc                                                                                         <  2:1009087/101‑1 (MQ=255)
                  gCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGGCg                                                                                    >  2:1901413/1‑101 (MQ=255)
                   cTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCgg                                                                                   <  1:3204039/101‑1 (MQ=255)
                    tCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.GAAGGGCGCCGTGggg                                                                                  >  2:1665567/1‑100 (MQ=255)
                     cGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGcc                                                                                 <  1:1832052/101‑1 (MQ=255)
                      gggCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGGCGGCCa                                                                                >  2:4489196/1‑101 (MQ=255)
                      gggCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCa                                                                                <  2:3522784/101‑1 (MQ=255)
                       ggCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCAGGGGG.AAAGGCC.CGGGCGGCCaa                                                                               >  1:4204926/1‑101 (MQ=255)
                          gcCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.GAAGGGCGCGGGCGGGCAAGGc                                                                            >  2:4621255/1‑101 (MQ=255)
                          gcCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.GAAAGCCGCGGGCGGCCAAGGc                                                                            >  1:2128804/1‑101 (MQ=255)
                           ccTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCGGCGGGGGGCCCAGGcc                                                                           >  2:2832120/1‑101 (MQ=255)
                            cTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGccc                                                                          <  2:1616682/101‑1 (MQ=255)
                            cTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGccc                                                                          <  1:1135650/101‑1 (MQ=255)
                             ttGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGGCGCGGGCGGctacggacgc                                                                         >  2:3068607/1‑92 (MQ=255)
                              tGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCAGGGGG.AAAGGCC.CGGGCGGCCAAGGgcccc                                                                        >  1:3465647/1‑99 (MQ=255)
                              tGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGGAAAAGGCCGGGGCGGGCCAGGGcccg                                                                         >  2:4273376/1‑100 (MQ=255)
                               gAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGGCGGGCAAGGcccccc                                                                       >  1:4280332/1‑98 (MQ=255)
                                 aGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGCCGAGGGGGGCCACGGgcccgccc                                                                     >  1:1446185/1‑96 (MQ=255)
                                 aGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTcg                                                                     <  2:615237/101‑1 (MQ=255)
                                  ggAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGCCTCGTGCGGCCCATGccccgctgc                                                                    >  1:1293323/1‑95 (MQ=255)
                                  ggAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTcgc                                                                    <  1:3443238/101‑1 (MQ=255)
                                     gacgacACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGGCGCGGGGGGGCAAAGCCCAGGCgcgcc                                                                 >  2:4893324/1‑100 (MQ=255)
                                     gacgacACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.CAACGCCGCCGCTCGCCCAGGccccccacccgc                                                                 >  2:1076475/1‑92 (MQ=255)
                                      acgacACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCtt                                                                <  2:293077/101‑1 (MQ=255)
                                        gacACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTgc                                                              <  2:4173189/101‑1 (MQ=255)
                                         acacCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTgcg                                                             <  1:3936103/101‑1 (MQ=255)
                                          cacCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCTGCGGGCGGCCCAGGCCCACTCCCGCCTgcgg                                                            >  1:4507550/1‑101 (MQ=255)
                                          cacCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTgcgg                                                            <  2:2793777/101‑1 (MQ=255)
                                          acccGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTgcgg                                                            <  2:1276577/99‑1 (MQ=255)
                                           acCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAAGCCGCGGGCGGGCAAGGCCCCGTCCCCCTTgcggg                                                           >  1:1002187/1‑101 (MQ=255)
                                            ccGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.GAAGGCCGCGGTCGGCCAAGGGCCCGTCGCGCTTgagatc                                                          >  2:3901916/1‑96 (MQ=255)
                                            ccGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTgcgggc                                                          <  1:2642848/101‑1 (MQ=255)
                                               gCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAAGCCGCGGGCGGCCAAGGCCCAGTCGGGGCTgcggggggg                                                       >  1:390405/1‑100 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATTCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGgag                                                   <  2:4936412/101‑1 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGCCGCGGGCGGCCAAGGCCCAGCCCCGCGTGCGGGGGGGgggg                                                   >  1:712082/1‑99 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGGCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTgggg                                                   >  2:2729235/1‑99 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGgag                                                   <  1:3411408/101‑1 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGgag                                                   <  2:135316/101‑1 (MQ=255)
                                                   aCAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGgag                                                   <  2:1627953/101‑1 (MQ=255)
                                                    cAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGCCGGCCAAAGCCCAGTCGCGCCGGCCGGCGGTGgcga                                                  >  1:556621/1‑101 (MQ=255)
                                                     aGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGagag                                                 <  2:3460486/101‑1 (MQ=255)
                                                      gACGGCCATCGGCCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAgc                                                <  2:2437938/101‑1 (MQ=255)
                                                      gACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAgc                                                <  1:2281573/101‑1 (MQ=255)
                                                        cGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAgcgc                                              <  1:1483398/101‑1 (MQ=255)
                                                        cGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAgcgc                                              <  2:3700019/101‑1 (MQ=255)
                                                         ggCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGcgcg                                             <  2:556414/101‑1 (MQ=255)
                                                          gCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGa                                            <  2:4792000/101‑1 (MQ=255)
                                                            cATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.AAAGGCCGCGGTCGGCCAAGGCCCCGGCGCGCGTGCGGGCGGGGGGGAGCGCGAcc                                          >  1:3813676/1‑101 (MQ=255)
                                                            cATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGAcc                                          <  2:3668291/101‑1 (MQ=255)
                                                              tCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTg                                        <  2:276274/101‑1 (MQ=255)
                                                               cGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGa                                       <  1:4043123/101‑1 (MQ=255)
                                                               cGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGa                                       <  2:1695592/101‑1 (MQ=255)
                                                                gaccggggacagcaGGACATGCATGGCCAGATCGGCA.GGCG.AGAGGCCGCGGTCGCCAAAGTCCCAGGCGTTTTTGCGGGCGGTGGAGAGCGCCACCTGaa                                      <  2:3934389/92‑1 (MQ=255)
                                                                gACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGGG.GAAGGGCCGGGCCCGCCAAAGCCCAGGCGCGCTTGCGGGCGGGGGGGAGCGCGACCTGaa                                      >  2:3197352/1‑101 (MQ=255)
                                                                gACCACGGGCAGCACGACATGCATGGCCAGATCGGCA.GGAG.AAAGGCCGCGGTCGGCCAAGGGCCCCTCGCGCTTGTGGGCGGGGGGGGGGGCGGCCCGaa                                      >  2:4547477/1‑101 (MQ=255)
                                                                 aCCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGaaa                                     <  2:2977329/101‑1 (MQ=255)
                                                                      gggCAGCACGACATGCATGGCCAGATCGGCA.GGCG.GAAAGCGGCGGTCGGCCAAGGCCCAGCCGCGGTCGCGGGCGGGGGGGAGTGCGGCCTGAAACATCg                                >  1:2837278/1‑101 (MQ=255)
                                                                       ggCAGCACGACATGCATGGCCAGATCGGCA.CGCG.AAAAGCCGCGGGCGGCCAAGGCCCCGTCGCGCTTGCGGGGGGGGGAGAGCGCGACCCGAAAAAcccg                               >  1:4491359/1‑99 (MQ=255)
                                                                          agcaCGACATGCATGGCCAGATCGGCA.GGGG.AAAAGCCGCGGGCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGGGGGGAGGGGGACCCGAAAAACCCGccc                            >  1:3901022/1‑99 (MQ=255)
                                                                           gcaCGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGcaca                           <  1:729200/101‑1 (MQ=255)
                                                                            caCGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcat                          <  1:4951308/101‑1 (MQ=255)
                                                                            caCGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcat                          <  1:2171456/101‑1 (MQ=255)
                                                                             aCGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcatc                         <  1:3554939/101‑1 (MQ=255)
                                                                               gACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcatcat                       <  1:1684688/101‑1 (MQ=255)
                                                                               gACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcatcat                       <  1:2000853/101‑1 (MQ=255)
                                                                               gACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcatcat                       <  2:3759649/101‑1 (MQ=255)
                                                                               gACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCAcatcat                       <  1:3273861/101‑1 (MQ=255)
                                                                                aCATGCATGGCCAGATCGGCA.GGCG.AAAGGGAGCCGTCGGCCAAGGGCCAGCCGCGCCTGCGGGCGGCGGAGAGCGCGACCCGGAACACCGGACCATCATa                      >  2:4081328/1‑101 (MQ=255)
                                                                                aCATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGGCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGGGGAGAGCGCGACCTGAAACACCGGCACATCATa                      >  2:1945633/1‑101 (MQ=255)
                                                                                 catgcatgGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAg                     <  2:379252/101‑1 (MQ=255)
                                                                                 catgcatgGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAg                     <  2:2552060/101‑1 (MQ=255)
                                                                                    gcatgGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCgg                  <  1:4093056/101‑1 (MQ=255)
                                                                                        ggCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCaa              <  1:2032002/101‑1 (MQ=255)
                                                                                         gCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCaaa             <  2:81861/101‑1 (MQ=255)
                                                                                           cAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGGGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAgg           >  1:3637760/1‑101 (MQ=255)
                                                                                             gATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGc         >  2:687300/1‑101 (MQ=255)
                                                                                                cGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtc      <  1:823624/101‑1 (MQ=255)
                                                                                                cGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtc      <  1:3323637/101‑1 (MQ=255)
                                                                                                  gCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtcgt    <  1:1901550/101‑1 (MQ=255)
                                                                                                  gCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtcgt    <  1:4397787/101‑1 (MQ=255)
                                                                                                  gCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtcgt    <  2:204272/101‑1 (MQ=255)
                                                                                                  gCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGcgtcgt    >  2:1750482/1‑101 (MQ=255)
                                                                                                   cA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGCGTCGTg   >  2:4224397/1‑101 (MQ=255)
                                                                                                    a.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGCGTCGTGc  <  2:3555215/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CCAGATCGGGGTCGCGCGGCTCGGGCGCCTTGAAGGAGACGACACCGGCGAACAGACGGCCATCGACCTCGGGCAGCACGACATGCATGGCCAGATCGGCA.GGCG.AAAGGCCGCGGTCGGCCAAGGCCCAGTCGCGCTTGCGGGCGGTGGAGAGCGCGACCTGAAACACCGGCACATCATAGCGGTCAAAGGGCGTCGTGC  >  NC_014034/2206253‑2206453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG