Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 612.4 179coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (91/88):  ref base (0/0):  total (91/88)

Only 100 of 207 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATG.C.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005
                                                                                                       |                                                                                               
aaTCTTGCGCAAACCGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCa                                                                                                  <  1:3953860/101‑1 (MQ=255)
     tGCGCAAACCTCGCCCGGACCCGCCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                            <  2:2725074/101‑4 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCCGCTCCCGCA.ggggc                                                                                            >  2:159246/1‑98 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.ggggc                                                                                            >  2:4853549/1‑98 (MQ=255)
      gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           <  2:2183115/101‑5 (MQ=255)
      gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           <  2:3803548/101‑5 (MQ=255)
      gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           >  1:2473089/1‑97 (MQ=255)
      gcgaAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           <  2:77489/101‑5 (MQ=255)
        ccAAAGCTCGCCCGATTCCGGCCTGCCACCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                                          <  2:2519334/100‑1 (MQ=255)
           aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                                       >  2:60020/1‑101 (MQ=255)
            aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                                      >  1:2523181/1‑101 (MQ=255)
             aCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                                     >  2:2543504/1‑101 (MQ=255)
              cTCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                    <  1:977032/101‑1 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGcc                                                                                  >  2:3532872/1‑101 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCCCAGGGGCTGCGCCTCGcc                                                                                  >  1:1088466/1‑101 (MQ=255)
                   ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                                               >  1:506154/1‑101 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             >  2:1966451/1‑101 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             >  1:2784044/1‑101 (MQ=255)
                       acccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           <  1:4277411/99‑1 (MQ=255)
                       acccGGCCGGCCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           <  2:3083358/99‑1 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           >  2:2628532/1‑101 (MQ=255)
                         ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                                         <  1:4115481/101‑1 (MQ=255)
                         ccGGCCTGCCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                                         <  1:3684678/101‑1 (MQ=255)
                            gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                      <  1:2740276/101‑1 (MQ=255)
                             ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCTCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                                     >  1:4370972/1‑101 (MQ=255)
                             ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCCGc                                                                     >  1:3130289/1‑101 (MQ=255)
                             ccTGCCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                                     <  2:4302346/101‑1 (MQ=255)
                               tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                                   >  2:591865/1‑101 (MQ=255)
                               tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                                   >  2:3322317/1‑101 (MQ=255)
                                gTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCggc                                                                  <  2:1286962/101‑3 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCC.GAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  1:4246878/101‑1 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  1:624535/101‑1 (MQ=255)
                                cgcacccGCCCTGCCGCAGCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  1:4510524/96‑1 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                                >  2:1232618/1‑101 (MQ=255)
                                     ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGGGCCTGCGTCGCGGc                                                             >  2:3344873/1‑101 (MQ=255)
                                      cgcccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                            <  1:3482132/98‑1 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                            <  1:4709747/101‑1 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGcagatcggaag                                                           >  1:770927/1‑91 (MQ=255)
                                          cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                                        <  1:4041239/101‑1 (MQ=255)
                                           tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                                       <  1:2085535/101‑1 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                      >  2:4435963/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAACAGCGCCTGCGTCGCGGCCGCGttt                                                      >  1:707899/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCCCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCCttt                                                      <  1:304956/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                    >  1:3268232/1‑101 (MQ=255)
                                               gCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                                   >  1:1692389/1‑101 (MQ=255)
                                               gCAACGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                                   >  2:4607544/1‑101 (MQ=255)
                                                 aaCGGCGTCGCCGGTGGCG.CCTGCAGG.CGGAAGACCGCCGTCCGCTCCCGCAGGGTCCGGGCCTCGCCCAGCAGCGCCTGCGGCGCGGGCGCGTTTTGCTc                                                 >  2:1317826/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTGCCGCAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGGTTTCCTc                                                 >  2:4363908/1‑101 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                                <  2:1824908/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                                <  1:3124360/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                                <  1:3937775/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGAGCCTCGCCCAGCAGCGCCTGCGGCGCGGCCGCGTTTTCCTCg                                                <  2:471214/101‑1 (MQ=255)
                                                   cggcggCGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                                               <  1:1370813/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              >  1:1940196/1‑101 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             <  1:683096/101‑1 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             >  2:3909909/1‑101 (MQ=37)
                                                     gcggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             >  1:4799363/1‑101 (MQ=37)
                                                      cggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                                            <  2:1492582/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           >  2:1702234/1‑101 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           >  1:4902892/1‑101 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  1:1005113/101‑1 (MQ=255)
                                                         cgcCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  1:1439524/101‑1 (MQ=25)
                                                         cgcCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  1:2413117/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                        <  1:2867365/101‑1 (MQ=25)
                                                           ccGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                       >  1:573408/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      >  2:2674383/1‑101 (MQ=25)
                                                             ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     >  2:142454/1‑101 (MQ=25)
                                                             ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     <  1:2028142/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     >  2:3288950/1‑101 (MQ=25)
                                                               tGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   >  1:3856847/1‑101 (MQ=21)
                                                               tGGCG.CCTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   >  2:4240703/1‑101 (MQ=25)
                                                                gggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   <  2:3408481/96‑1 (MQ=25)
                                                                 ggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  2:3949090/97‑1 (MQ=25)
                                                                 ggcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCCGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  >  2:2570139/5‑101 (MQ=21)
                                                                  gcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  1:3193565/98‑1 (MQ=25)
                                                                  gcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  1:3172751/98‑1 (MQ=25)
                                                                  gcgccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  1:2243237/98‑1 (MQ=25)
                                                                    gccTGCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                               <  1:282353/100‑1 (MQ=25)
                                                                        gCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           >  2:3663920/1‑101 (MQ=21)
                                                                        gCATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           >  2:4675867/1‑101 (MQ=21)
                                                                         cATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                          <  1:1821368/101‑1 (MQ=25)
                                                                         cATG.CGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                          <  2:2905689/101‑1 (MQ=25)
                                                                              cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                      <  2:707810/99‑1 (MQ=25)
                                                                              cggAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggggaac                      >  2:2033939/3‑97 (MQ=21)
                                                                               ggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc                     <  2:491210/100‑1 (MQ=21)
                                                                                 aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  1:3072427/101‑1 (MQ=21)
                                                                                 aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   >  2:2709706/1‑101 (MQ=21)
                                                                                 aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   >  1:1750586/1‑101 (MQ=21)
                                                                                 aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  1:2574496/101‑1 (MQ=21)
                                                                                    accgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                >  2:3996991/11‑101 (MQ=21)
                                                                                    accgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                >  2:4818339/11‑101 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               <  1:2970930/92‑1 (MQ=21)
                                                                                      cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa              <  1:154637/93‑1 (MQ=21)
                                                                                      cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa              <  2:2221595/93‑1 (MQ=21)
                                                                                       gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  1:1263593/8‑101 (MQ=25)
                                                                                         cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt           >  1:219852/6‑101 (MQ=255)
                                                                                            cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        >  2:402512/3‑101 (MQ=255)
                                                                                            cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        >  1:4200834/3‑101 (MQ=255)
                                                                                              gCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      <  2:2427215/101‑1 (MQ=255)
                                                                                              gCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      >  2:3128784/1‑101 (MQ=255)
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AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATG.C.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG