Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,056 +T 3.2% intergenic (+104/‑138) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0561.→T 3.2% 12.7 ‑0.9 ‑0.6154intergenic (+104/‑138)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (86/63):  total (90/64)
Fisher's exact test strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.68e-01

Only 100 of 167 total aligned reads displayed.

GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493956‑2494152
                                                                                                      |                                                                                                        
ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGaa                                                                                                           <  2:1670615/101‑1 (MQ=255)
 gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.t                                                                                                         <  1:2724890/101‑1 (MQ=255)
 gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.t                                                                                                         <  1:231201/101‑1 (MQ=255)
  ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.Tg                                                                                                        <  2:4312072/101‑1 (MQ=255)
   gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCC.GGGGGGTGAA.Tgg                                                                                                       <  1:2876840/101‑1 (MQ=255)
   gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.Tgg                                                                                                       >  2:4718543/1‑101 (MQ=255)
     gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..g                                                                                                   >  2:2348482/1‑101 (MQ=255)
       tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aa                                                                                                >  1:2942809/1‑100 (MQ=255)
        gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aag                                                                                               >  1:4935839/1‑99 (MQ=255)
        ccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aag                                                                                               <  2:295632/100‑3 (MQ=255)
         cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagcc                                                                                             <  1:3490442/101‑5 (MQ=255)
         cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagcc                                                                                             <  1:1753572/101‑5 (MQ=255)
         cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagcc                                                                                             <  2:3848104/101‑5 (MQ=255)
          ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagcca                                                                                            >  2:1580692/1‑96 (MQ=255)
               gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCC.GGGGGGCGAA.TGGC..G.aagccagagg                                                                                        >  2:4054418/2‑92 (MQ=18)
               gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagccagagg                                                                                        <  2:4234184/100‑10 (MQ=255)
                tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.aagccagaggg                                                                                       <  1:1113887/101‑11 (MQ=255)
                     aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                                                  >  2:2286492/1‑101 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGATATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                               >  2:5013465/1‑98 (MQ=17)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCaggg                                                                               >  2:2108998/1‑98 (MQ=18)
                         ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GTGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                              >  1:4296424/1‑97 (MQ=17)
                         ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                              >  2:305709/1‑97 (MQ=17)
                            cTCCCCCCGGGATATTTGCAGCAAGTTGAAACCGGATGCCCTTCATCTTGCCCGAAATATCCC.GGGGGGTGAG.TCGC..GAAG.CCACAGGGGGGC.AGCG.c.ccc                                                                        >  2:1792817/1‑101 (MQ=17)
                             tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cccc                                                                       >  2:3924517/1‑101 (MQ=17)
                              ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccc                                                                      <  2:4222518/101‑2 (MQ=17)
                                   cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGccc                                                                 <  2:1778835/101‑1 (MQ=17)
                                    gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGcccc                                                                >  2:3722405/1‑101 (MQ=17)
                                     ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCC.TGCCCcg                                                               >  1:414303/1‑101 (MQ=2)
                                      gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCC.TGCCCcgc                                                              >  1:1106325/1‑100 (MQ=2)
                                      gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.CCCC.TGCCCcgg                                                              >  1:1500262/1‑101 (MQ=18)
                                      gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.C.CCCC.TGCCCcgg                                                              >  2:3780334/1‑101 (MQ=17)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCC.TGCCCcgcc                                                             >  2:190218/1‑99 (MQ=2)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCC.TGCCCcgcc                                                             <  2:2567939/101‑3 (MQ=2)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC..AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCC.TGCCCcgcc                                                             >  1:831521/1‑99 (MQ=2)
                                       atatTTGCAGGAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.GGGC..GAAG.CCAGAGGGGGGCCGGGC.C.CCCC.TGCCCcgcc                                                             >  1:1654281/1‑99 (MQ=17)
                                       atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCcgcg                                                             >  1:2509077/1‑99 (MQ=17)
                                       atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCcggc                                                             >  1:1947906/1‑101 (MQ=17)
                                          tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGt                                                          <  2:1530092/101‑1 (MQ=17)
                                           ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTc                                                         <  1:1445728/101‑1 (MQ=17)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGGcc                                                        >  1:3779656/1‑101 (MQ=17)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGCcc                                                        >  1:3021628/1‑101 (MQ=17)
                                            tGCACNNAGATGCAACCGGATTCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTcc                                                        <  1:1976333/101‑1 (MQ=14)
                                              cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCcggcggggcgt                                                      >  2:4463382/1‑96 (MQ=17)
                                                gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTgg                                                    >  1:4272173/1‑101 (MQ=17)
                                                   aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTggcgg                                                 >  2:3403828/1‑101 (MQ=18)
                                                   aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCCGCGGTCCGTggcgg                                                 >  2:3144664/1‑101 (MQ=17)
                                                    gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTggcggc                                                >  1:991197/1‑101 (MQ=17)
                                                        aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCt                                           >  2:29184/1‑101 (MQ=17)
                                                        aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCt                                           >  1:3099186/1‑101 (MQ=18)
                                                         aaCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCtt                                          <  2:2912032/101‑1 (MQ=17)
                                                          aCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTg                                         >  2:3661741/1‑101 (MQ=18)
                                                          aCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTg                                         <  1:4969488/101‑1 (MQ=17)
                                                          aCCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTg                                         <  1:1317686/101‑1 (MQ=17)
                                                           ccGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGa                                        >  2:2153091/1‑101 (MQ=18)
                                                           ccGGATGCCTTTCATCTTCCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGa                                        <  2:1609947/101‑1 (MQ=17)
                                                            cGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAc                                       >  1:4841375/1‑101 (MQ=17)
                                                             ggATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcg                                      <  2:3017367/101‑1 (MQ=17)
                                                              gATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcgg                                     >  1:4469323/1‑101 (MQ=18)
                                                              gATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCA.CACCCC.TGCCCCGGCGGTCCGTGGCGGGG.CCCTGAcgg                                     >  1:836473/1‑101 (MQ=12)
                                                                tGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGAcgggc                                   <  1:740217/101‑1 (MQ=17)
                                                                tGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGT.GCGGCG.CCTTGAcgggcg                                  >  2:218413/1‑101 (MQ=17)
                                                                    tttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCgggg                               >  2:1200603/1‑101 (MQ=18)
                                                                    tttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCgggg                               <  2:4831782/101‑1 (MQ=17)
                                                                     ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                              >  2:76190/1‑101 (MQ=18)
                                                                     ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                              >  2:2277111/1‑101 (MQ=18)
                                                                     ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACCGGCggggg                              >  2:2228155/1‑101 (MQ=17)
                                                                     ttCATCTTGCCCGAAATATCCC.GGGGGGTGAA.CGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCggggg                              >  2:1903303/1‑101 (MQ=18)
                                                                      tCATCTTGCCCGAAATATCCC.GGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TCCCCCGGCGGTCCGTGGCGGCG.CCTTGACCGGGgggggg                             >  2:1239428/1‑100 (MQ=9)
                                                                       cATCTTGCCCGAAATATCCC.GGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGgcg                            >  1:531678/1‑101 (MQ=18)
                                                                        aTCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGgcgg                           >  1:2168370/1‑100 (MQ=18)
                                                                          cTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCgg                         >  1:3041789/1‑101 (MQ=18)
                                                                          cTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCgg                         <  2:4405102/101‑1 (MQ=18)
                                                                            tGCCCGAAATATCCCGGGGGGGGGAA.TGGG..G.AA.CCAAGGGGGGGGAAGGG.C.CCCCTTGCCCCGCCGGCCCG.GGCGGGGCCCTTGACGGGGGGGGGGGGggg                        >  1:2060910/1‑101 (MQ=255)
                                                                            tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.GCTTGACGGGCGGGGGCGCGGGc                       >  2:4999236/1‑101 (MQ=18)
                                                                            tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGc                       <  1:3325569/101‑1 (MQ=18)
                                                                            tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGc                       <  2:1954602/101‑1 (MQ=18)
                                                                            tGCCCGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGc                       <  1:380383/101‑1 (MQ=18)
                                                                             gCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AA.GCCAGAGGGGGCCAGCGCC.CCCC.TCCCCCGGCGGTCCGTGGCGGCG.CCTTCACCTGGGGGGGCGCggggc                      >  2:621723/1‑99 (MQ=9)
                                                                              cccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAt                     <  1:2606143/101‑1 (MQ=18)
                                                                              cccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAt                     <  1:2381922/101‑1 (MQ=18)
                                                                               ccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:2961129/101‑1 (MQ=18)
                                                                               ccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAtt                    >  2:3021597/1‑101 (MQ=18)
                                                                               ccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:2861406/101‑1 (MQ=18)
                                                                               ccGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCAtt                    >  2:2835915/1‑101 (MQ=18)
                                                                                cgcaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTa                   <  2:4256508/98‑1 (MQ=18)
                                                                                cGAAATATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTGGACGGGCGGGGGCGCgggactga                   >  2:3170134/1‑96 (MQ=17)
                                                                                  aaaTATCCC.GTGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGGGCGGGCATTAAc                 >  1:3450778/1‑101 (MQ=18)
                                                                                  aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  2:4202196/1‑101 (MQ=18)
                                                                                  aaaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  1:3127500/1‑101 (MQ=18)
                                                                                   aaTATCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGAGCC.CCCC.TGCCCCGGCGGACAGCGGCGGAG.ACTTGACGGGGGGGGGCCCGGGCGTCAAcc                >  1:4022190/1‑101 (MQ=2)
                                                                                     tatCCG.GGGGGGTGAA.TGCC..GAAG.CCAGAGGGGGGCAGCCC.C.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGa              <  1:2004203/101‑1 (MQ=25)
                                                                                     tatCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  1:4144164/1‑101 (MQ=21)
                                                                                      atCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             <  2:551369/101‑1 (MQ=21)
                                                                                       tCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            >  1:3623977/1‑101 (MQ=21)
                                                                                       tCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  2:333362/101‑1 (MQ=21)
                                                                                       tCCC.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:4718866/101‑1 (MQ=21)
                                                                                        ccc.GGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt           <  1:234970/101‑1 (MQ=21)
                                                                                            ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        <  1:4782575/101‑1 (MQ=25)
                                                                                              ggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa      <  2:3452673/101‑1 (MQ=25)
                                                                                               gggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     >  2:2360260/1‑101 (MQ=25)
                                                                                                      |                                                                                                        
GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCC.GGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCC.TGCCCCGGCGGTCCGTGGCGGCG.CCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493956‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG