Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 4.7% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 4.7% 37.6 ‑0.7 ‑0.7277intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (166/98):  total (172/105)
Fisher's exact test strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-01

Only 100 of 309 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379
                                                                                                                                                                                                        
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                    >  1:2060499/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                    >  1:1336744/1‑101 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                <  2:1643892/101‑2 (MQ=255)
       gTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcg                                                                                              >  1:3234360/1‑98 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                          >  1:716576/1‑101 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCAATAGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                          >  1:4321076/1‑101 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        >  2:2161256/1‑101 (MQ=255)
            ttccttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        <  2:2752184/98‑1 (MQ=255)
             ttcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                       >  2:555704/1‑101 (MQ=255)
             atcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCGCGCGGGAccg                                                                                       <  2:4095639/100‑1 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                      >  1:537014/1‑101 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                      >  1:3623557/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                    >  1:3464546/1‑101 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   >  2:1800230/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                  >  2:77189/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                  >  1:1651394/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGGTTTTT.CCCCCGCGGGGCCGCCcgcg                                                                                 >  2:3273916/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcgcg                                                                                 >  2:3858005/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcgcg                                                                                 >  2:49494/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCCGGACCGCCcgcg                                                                                 >  2:1250242/1‑101 (MQ=255)
                    tACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcgg                                                                                <  1:3049610/101‑1 (MQ=255)
                     aCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGCCTCTAGCCGATGGCCGGGGCGGCGACAGGATTTTT.CCCCCGCTGGACCGCCCgcggc                                                                               >  2:4813496/1‑101 (MQ=255)
                      cGATCGACTGCCCCCGCAGTCTGAACCCATTGGTTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggcg                                                                              <  2:823676/101‑1 (MQ=255)
                      cGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggcg                                                                              >  2:924198/1‑101 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGcggcgg                                                                             >  1:2767572/1‑101 (MQ=255)
                        aTCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCggg                                                                            >  1:997317/1‑101 (MQ=255)
                          cGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGTCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgc                                                                          >  1:2476237/1‑101 (MQ=255)
                          cGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCggggg                                                                          >  1:2220240/1‑100 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                         >  2:2854336/1‑101 (MQ=255)
                             cTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCa                                                                       <  1:1775211/101‑1 (MQ=255)
                             cTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCa                                                                       >  2:1514280/1‑101 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCaa                                                                      <  2:2041739/101‑1 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAc                                                                     >  2:3037778/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAc                                                                     >  2:2418387/1‑101 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTcc                                                                 >  2:1893582/1‑101 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  2:1970148/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  1:183272/1‑97 (MQ=255)
                                          cTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTc                                                         >  2:3056076/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCGACCT.CCGCCCGTcc                                                        >  1:4702803/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  1:2327983/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                       >  1:3935194/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                       >  1:3973721/1‑101 (MQ=255)
                                             aaccggttGGTTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  2:2199416/95‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  1:4390152/101‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      >  2:359783/1‑100 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      >  1:300104/1‑100 (MQ=255)
                                             aaCCCATGGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  2:1934372/101‑2 (MQ=255)
                                               cccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccccgc                                                    >  2:4848404/1‑99 (MQ=255)
                                                  aTTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggg                                                >  1:1901617/1‑101 (MQ=255)
                                                  aTTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggg                                                >  2:2225225/1‑101 (MQ=255)
                                                   ttGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgggg                                               >  1:1032831/1‑101 (MQ=255)
                                                    tGGCGTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGA.TTTTGCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              <  1:833227/101‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             >  2:253133/1‑101 (MQ=255)
                                                      gCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccaccggcggac                                             >  2:1458235/1‑91 (MQ=255)
                                                        ttttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTCCCC.GCGGGGGACtg                                          >  1:2151364/1‑101 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgt                                         <  2:3740498/101‑1 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgt                                         >  1:4516810/1‑101 (MQ=255)
                                                          ttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgtg                                        <  1:3835600/101‑1 (MQ=255)
                                                          ttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgtg                                        >  1:201411/1‑101 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACATGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                       <  1:3498948/101‑1 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                       <  2:368584/101‑1 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                       >  1:1817028/1‑101 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                       <  1:1800363/101‑1 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                       >  2:3494925/1‑101 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGc                                      <  2:4345014/101‑1 (MQ=255)
                                                             cTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCa                                     <  1:1194880/101‑1 (MQ=255)
                                                              tGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaa                                    <  1:3316968/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   >  1:4822339/1‑101 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                 >  1:2209489/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                               >  2:5024721/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                             <  1:3688636/101‑1 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                            <  2:4018633/101‑1 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                            <  2:3443603/101‑1 (MQ=255)
                                                                       ccGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGCCGCCCCCCGCCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGCGGCAACGGGGcacc                           >  1:1282344/1‑101 (MQ=255)
                                                                       ccGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacc                           <  1:2682943/101‑1 (MQ=255)
                                                                        cGATGGCCGGGGGTGCAACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          <  1:3380638/101‑1 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          >  2:4468940/1‑101 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          >  1:927347/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         >  2:2360704/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         <  2:3298890/101‑1 (MQ=255)
                                                                          aTGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcg                        >  2:4660598/1‑101 (MQ=255)
                                                                             gCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCgg                     >  1:4969074/1‑101 (MQ=255)
                                                                              ccGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGt                    <  1:4652346/101‑1 (MQ=255)
                                                                                 ggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTc                 >  1:4207992/1‑101 (MQ=255)
                                                                                 ggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGGTTc                 >  1:694139/1‑101 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                >  2:3194221/1‑101 (MQ=255)
                                                                                   ggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGc               >  1:2600079/1‑101 (MQ=255)
                                                                                    gggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCt              >  1:406922/1‑101 (MQ=255)
                                                                                    gggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCt              >  2:260573/1‑101 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt            >  2:3381718/1‑101 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           <  1:2854549/101‑1 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         >  1:2417424/1‑101 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         <  1:4719814/101‑1 (MQ=255)
                                                                                          cAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGcc        <  1:3402826/101‑1 (MQ=255)
                                                                                          cAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGcc        <  2:1106999/101‑1 (MQ=255)
                                                                                          cAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGcc        >  1:3243360/1‑101 (MQ=255)
                                                                                                ttttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTc   >  1:610265/1‑101 (MQ=255)
                                                                                                ttttCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTc   <  2:678967/101‑1 (MQ=255)
                                                                                                 tttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCg  <  2:2209334/101‑1 (MQ=255)
                                                                                                 tttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCg  <  2:610186/101‑1 (MQ=255)
                                                                                                                                                                                                        
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG