Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,173,548	+C	100%	intergenic (+583/‑99)	tyrA → / → RCAP_rcc02983	prephenate dehydrogenase/extensin family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,173,548	1	.→C	100.0%	146.5	40	intergenic (+583/‑99)	tyrA/RCAP_rcc02983	prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (25/13):  ref base (1/0):  total (27/13)

TCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CC.GCCGCGCAAGCGCAA.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173455‑3173642                                                                                               |                                                                                                 tCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.cccgccg                                                                                           <  2:771726/101‑6 (MQ=255)      tCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCgcgcaa                                                                                      <  1:2737931/101‑1 (MQ=255)        ggCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGGAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCgcgcaagc                                                                                    >  1:4383859/1‑101 (MQ=255)             cgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCgcgcaagcgcaac                                                                               <  1:2978213/101‑2 (MQ=255)               ccGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCgcgcaagcgcaacgc                                                                             <  2:4283371/101‑4 (MQ=255)                  gccccgcccgccCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAA.Cgcccc                                                                         <  1:2567884/97‑1 (MQ=255)                  gTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAA.Cgcccc                                                                         >  2:2567703/1‑101 (MQ=255)                         ccccccccgcccccGCCTCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCccccgccccg                                                                   <  2:3254149/98‑1 (MQ=255)                          cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCgg                                                                  <  2:2934412/101‑1 (MQ=255)                            cccccgcccccACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGaa                                                                <  2:4383542/101‑1 (MQ=255)                              cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAgc                                                              >  2:1181809/1‑101 (MQ=255)                                 gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAgcgcc                                                           <  1:1406966/101‑1 (MQ=255)                                 gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAgcgcc                                                           <  1:5005176/101‑1 (MQ=255)                                     ccaccaCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAgcgcccgcg                                                       >  1:4616016/1‑101 (MQ=255)                                        ccccccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAgcgcccgcgccc                                                    <  1:184609/96‑1 (MQ=255)                                           cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGaa                                                 >  2:1033677/1‑101 (MQ=255)                                            tCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                >  2:1577758/1‑101 (MQ=255)                                                ccgatctggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcccc                                            <  1:4210939/94‑1 (MQ=255)                                                       ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccccagatcgg                                     >  2:4210622/1‑94 (MQ=255)                                                          aGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGc                                  >  2:4441294/1‑101 (MQ=255)                                                                cgccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGcc                            <  1:1181926/101‑1 (MQ=255)                                                                 gccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                           <  2:4615694/101‑1 (MQ=255)                                                                 gccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                           >  2:1814469/1‑101 (MQ=255)                                                                  ccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGa                          >  2:307299/1‑101 (MQ=255)                                                                    gggCGGCGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCCCGCCTGACGCCCCTCCGTCCCGGAGGGCCGacc                        >  2:4652578/1‑99 (MQ=255)                                                                    gggCGGAGTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGc                        >  2:4500980/1‑101 (MQ=255)                                                                       cggcgTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                     >  1:3482847/1‑101 (MQ=255)                                                                        ggcgTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  2:2916619/1‑101 (MQ=255)                                                                        ggcgTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  1:2543605/1‑101 (MQ=255)                                                                          cgTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:2494432/1‑101 (MQ=255)                                                                          cgTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:2999169/1‑101 (MQ=255)                                                                           gTGCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCCGGGCCGAGCCCGGCCt                 >  2:1221545/1‑101 (MQ=255)                                                                             gCTGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGCCCCGCAGGGCCGAGCCCGGCCTgc               >  2:4673441/1‑101 (MQ=255)                                                                             gCTGTCGCGCCTTTTCGGACC.GCCGAGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGACCCTCCGGCCCGCAGGGACGAGGCCGGCCTgc               >  2:563303/1‑101 (MQ=255)                                                                             gCTGTCCCGCCTTTTCGG.CCTGCCGCGCAAGCCCAACGCCCCCGCCCCGGAAGCCCCCCCGCCCGAACCCACTCACGCCCCCAGGCCCGAGCCCGGCCTgc               >  2:3013478/1‑101 (MQ=255)                                                                               tGTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  1:4967915/1‑101 (MQ=255)                                                                                gTCGCGCCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg            >  2:1213532/1‑101 (MQ=255)                                                                                   gcgcCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGGCTGCGCggga         >  2:4295984/1‑100 (MQ=255)                                                                                     gcCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg       >  1:2658854/1‑101 (MQ=255)                                                                                     gcCTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg       >  2:1495008/1‑101 (MQ=255)                                                                                      ccTTTTCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg      >  2:2287146/1‑101 (MQ=255)                                                                                          ttCGGCCC.GCCGCGCAAGCGCAACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg  >  1:1695877/1‑101 (MQ=255)                                                                                               |                                                                                                 TCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CC.GCCGCGCAAGCGCAA.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173455‑3173642 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG