BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,256,180	+C	100%	coding (1323/1734 nt)	recJ →	single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,256,180	1	.→C	100.0%	521.6	139	coding (1323/1734 nt)	recJ	single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): new base (71/67): ref base (0/1): total (71/68)

Only 100 of 148 total aligned reads displayed.


GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CC.GCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.G.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270


gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGGTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.c                                                                                                        >  1:3553612/1‑101 (MQ=255)


gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.c                                                                                                        <  2:506839/101‑1 (MQ=255)


gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.c                                                                                                        <  1:4023508/101‑1 (MQ=255)


 ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.cc                                                                                                       <  1:1094248/101‑1 (MQ=255)


 ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.cc                                                                                                       <  2:1974487/101‑1 (MQ=255)


   tgcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.cccg                                                                                                     <  1:4031483/100‑3 (MQ=255)


   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.A.cccg                                                                                                     >  1:1901971/1‑99 (MQ=255)


         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCTTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.g                                                                                              <  1:4494680/101‑1 (MQ=255)


         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.g                                                                                              >  1:4279718/1‑101 (MQ=255)


         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGGCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.g                                                                                              >  2:1875479/1‑101 (MQ=255)


         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGAGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.g                                                                                              >  2:3448078/1‑101 (MQ=255)


          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gc                                                                                             >  1:2274022/1‑100 (MQ=255)


           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gca                                                                                            <  1:4915236/101‑3 (MQ=255)


           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gca                                                                                            <  2:80696/101‑3 (MQ=255)


           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gca                                                                                            <  1:1185785/101‑3 (MQ=255)


             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacg                                                                                          <  1:1785388/101‑5 (MQ=255)


             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacg                                                                                          >  1:158663/1‑97 (MQ=255)


              aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacgg                                                                                         <  2:4254346/101‑6 (MQ=255)


                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacggct                                                                                       <  2:3935799/101‑8 (MQ=255)


                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacggctg                                                                                      <  2:1500099/101‑9 (MQ=255)


                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacggctgg                                                                                     >  2:2616698/1‑92 (MQ=255)


                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATg.gcacggctgg                                                                                     >  1:94344/1‑92 (MQ=255)


                        tctcGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.t                                                                            <  1:2472541/98‑1 (MQ=255)


                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.G.CACGGCTGGCCGA.g.ttg                                                                          <  2:4006499/101‑3 (MQ=255)


                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.ttgct                                                                        >  1:2555655/1‑97 (MQ=255)


                             aGGGGCTTTTGCTTAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.G.CACGGCTGGCCGA.g.ttgctc                                                                       <  2:1413648/101‑6 (MQ=255)


                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.ttgctc                                                                       >  2:4666067/1‑96 (MQ=255)


                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.ttgctc                                                                       <  1:3453541/101‑6 (MQ=255)


                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.ttgctc                                                                       >  1:2575333/1‑96 (MQ=255)


                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.g.ttgctcgcc                                                                    <  2:1901834/101‑9 (MQ=255)


                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGccc                                                                    >  1:2459081/1‑101 (MQ=255)


                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGccc                                                                    >  2:4043266/1‑101 (MQ=255)


                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.G.CACGGCTGGCCGA.GTTGCTCGccc                                                                    <  2:2214537/101‑1 (MQ=255)


                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                  <  1:2812114/101‑1 (MQ=255)


                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                 >  1:4629387/1‑101 (MQ=255)


                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                                 <  1:1201644/101‑1 (MQ=255)


                                     ttGCTCAAGGGCTGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCCGCa                                                                >  1:4128457/1‑101 (MQ=255)


                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGcc                                                                >  2:1292603/1‑100 (MQ=255)


                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                               >  1:1374178/1‑101 (MQ=255)


                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                               <  2:2950074/101‑1 (MQ=255)


                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.G.CACGGCTGGCCGA.GTTGCTCGCCCGGCAgg                                                              <  1:51266/101‑1 (MQ=255)


                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                             <  1:3983003/101‑1 (MQ=255)


                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc                                                            <  1:897208/101‑1 (MQ=255)


                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc                                                            <  1:1870849/101‑1 (MQ=255)


                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc.g                                                          >  1:3508661/1‑101 (MQ=255)


                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgg.g.c                                                        >  2:2618333/1‑101 (MQ=255)


                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGGC.G.C.g.gg                                                   >  1:1062966/1‑100 (MQ=255)


                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGG.GTTGCCCGCCCGGCAgggggggggg                                                      >  2:1390850/1‑94 (MQ=255)


                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.g.ggc                                                  <  1:4290424/101‑3 (MQ=255)


                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.g.ggcac                                                <  1:3410614/101‑5 (MQ=255)


                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.g.gggacg                                               >  2:1124347/1‑96 (MQ=255)


                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.c.gg                                             <  2:4779617/101‑1 (MQ=255)


                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.c.gg                                             >  1:3281301/1‑101 (MQ=255)


                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.G.CGG.GCA.c.gggg                                           >  1:3980762/1‑99 (MQ=255)


                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.c.gggc                                           <  2:4816164/101‑3 (MQ=255)


                                                      gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.g.ggcccgggcg                                           >  1:737715/1‑92 (MQ=255)


                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.c.gggcgg                                         <  1:314654/101‑5 (MQ=255)


                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.g.gggccgggggg                                          >  1:3083632/1‑91 (MQ=255)


                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.c.gggcggc                                        <  1:1011539/101‑6 (MQ=255)


                                                            cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGG.G.G.GCA.CGGGgggcccgg                                     >  1:1322353/1‑101 (MQ=255)


                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGcggcccggcc                                   <  2:43663/101‑1 (MQ=255)


                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GGA.CGGGCGGGCCGGCCg                                  >  2:2069807/1‑101 (MQ=255)


                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCg                                  >  1:2145889/1‑101 (MQ=255)


                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGACC.CC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGAt                                <  1:2787319/101‑1 (MQ=255)


                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGAt                                <  2:1810577/101‑1 (MQ=255)


                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATGAC.AC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATcc                              >  2:699078/1‑100 (MQ=255)


                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGATGGAGC.CC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCt                              <  1:4954745/101‑1 (MQ=255)


                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTg                             <  1:1755191/101‑1 (MQ=255)


                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTg                             >  1:1526449/1‑101 (MQ=255)


                                                                     gggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCCGCCGATCTgg                            >  2:1363505/1‑100 (MQ=255)


                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                           <  1:2635195/101‑1 (MQ=255)


                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                           <  2:4682627/101‑1 (MQ=255)


                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                           >  2:4142690/1‑101 (MQ=255)


                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                           >  2:1327489/1‑101 (MQ=255)


                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCg                           <  1:159393/101‑1 (MQ=255)


                                                                       gTCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGGCCGGCCGATCTGCgg                          >  1:1524351/1‑101 (MQ=255)


                                                                        tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAGGGC.G.C.GCGCACC.GGGGGGCCGGCCGCTTTGCCGc                         >  2:3509414/1‑101 (MQ=255)


                                                                        tCTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGc                         >  2:4925906/1‑101 (MQ=255)


                                                                         cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                        >  1:1306090/1‑101 (MQ=255)


                                                                         cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCt                        >  1:1276473/1‑101 (MQ=255)


                                                                         cTGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCCGCCCGTCTGCgggc                        >  2:2808572/1‑99 (MQ=255)


                                                                          tGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCTGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCtt                       <  2:1593756/101‑2 (MQ=255)


                                                                          tGACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGGGGCCCCGCCGGTCTGCGGGTg                       >  1:802149/1‑101 (MQ=255)


                                                                           gACCCTGGCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                      >  1:4518389/1‑101 (MQ=255)


                                                                                tggCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                 <  1:624457/99‑1 (MQ=255)


                                                                                 ggCCGAGGCGCAGCTGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCC.CGGGGGGCCCGGCCCATCTGCGGCTGGGCGGGc                >  1:4870866/2‑101 (MQ=255)


                                                                                     gaggcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCt            <  2:2201612/91‑1 (MQ=255)


                                                                                      gggcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  1:4546943/92‑1 (MQ=255)


                                                                                      aggcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGCCCTg           >  2:1791275/10‑101 (MQ=255)


                                                                                      aggcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  2:4047142/92‑1 (MQ=255)


                                                                                       ggcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGa          >  2:3497188/9‑101 (MQ=255)


                                                                                        gcgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  1:4143007/94‑1 (MQ=255)


                                                                                        gcgcagctGGA.A.CCAGCGATG.GCAG.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  2:4433272/94‑1 (MQ=255)


                                                                                         cgcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGTCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg        <  2:158647/95‑1 (MQ=255)


                                                                                          gcagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc       <  1:3096687/96‑1 (MQ=255)


                                                                                           cagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCC.G.GCA.CGGGCGGCCCGGCCGATCTGCGGGTGGGACGGCTCCCTATGcc      >  2:354160/5‑101 (MQ=255)


                                                                                           cagctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  2:2801378/97‑1 (MQ=255)


                                                                                            agctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCg     >  1:4955129/4‑101 (MQ=255)


                                                                                              ctGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggg   >  2:333202/2‑101 (MQ=255)


                                                                                               tGGA.ACCC.GCGATG.GCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGG.GCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCgggg  >  2:2793437/1‑100 (MQ=255)


GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGA.A.CC.GCGATG.G.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.G.C.G.GCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG