BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,258,963	+C	100%	coding (778/1971 nt)	efeU →	ferrous iron permease EfeU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,258,963	1	.→C	100.0%	47.9	191	coding (778/1971 nt)	efeU	ferrous iron permease EfeU
Reads supporting (aligned to +/- strand): new base (98/76): ref base (0/17): total (98/93)
Fisher's exact test strand distribution p-value = 2.13e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.08e-01

Only 100 of 211 total aligned reads displayed.


AATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258864‑3259058


aaTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.c                                                                                                >  1:779551/1‑101 (MQ=255)


  tGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccc                                                                                              <  2:613587/101‑2 (MQ=255)


   gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             >  1:1145741/1‑99 (MQ=255)


   gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             <  1:408550/101‑3 (MQ=255)


      tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                          >  1:3817137/1‑96 (MQ=255)


      tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                          <  2:2807001/101‑6 (MQ=255)


      tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                          >  1:5048544/1‑96 (MQ=255)


      tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                          <  1:863672/101‑6 (MQ=255)


       cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  1:4803546/1‑101 (MQ=255)


        ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         >  1:3490684/1‑101 (MQ=255)


         gTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGct                                                                                        >  2:1041563/1‑101 (MQ=255)


         gTCGCGGGCACCGTGCTTTAGGCGCTCTTTGGCTATCACGACAGCCCGACCCCGGGCGAGGTAGACCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGct                                                                                        >  1:1254647/1‑101 (MQ=255)


           cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      >  2:744634/1‑101 (MQ=255)


            gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                     >  2:3564453/1‑101 (MQ=255)


             cgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTc                                                                                    >  2:1221144/1‑101 (MQ=255)


              ggtcACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCGGGCCGTG.ACCCTGCCGCTCTTcc                                                                                   <  2:546792/98‑1 (MQ=255)


              gggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTcc                                                                                   <  2:1062496/101‑1 (MQ=255)


              gggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTcc                                                                                   >  2:2597537/1‑101 (MQ=255)


                gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  1:1558690/101‑1 (MQ=255)


                gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  2:4672689/101‑1 (MQ=255)


                   ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                              >  2:2266482/1‑101 (MQ=255)


                   ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                              <  1:1048473/101‑1 (MQ=255)


                   ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                              >  1:274583/1‑101 (MQ=255)


                    cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  1:4578237/1‑101 (MQ=255)


                    cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  2:784463/1‑101 (MQ=255)


                    cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  2:1829008/1‑101 (MQ=255)


                     gTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGccc                                                                            <  2:1484607/101‑1 (MQ=255)


                     gTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGccc                                                                            <  1:3542429/101‑1 (MQ=255)


                        cTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgc                                                                         <  1:3252683/101‑1 (MQ=255)


                        cTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgc                                                                         >  1:1852252/1‑101 (MQ=255)


                         tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        >  1:3562514/1‑101 (MQ=255)


                         tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        >  1:5039965/1‑101 (MQ=255)


                          ttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCgcgc                                                                       <  2:1102620/101‑1 (MQ=255)


                          ttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCgcgc                                                                       >  1:2460129/1‑101 (MQ=255)


                            cGGCGCTCTTTGGCTATCACGACAGCCCGCCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  1:4854659/101‑1 (MQ=255)


                            cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     >  1:3209614/1‑101 (MQ=255)


                             ggCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccg                                                                    >  2:2523817/1‑101 (MQ=255)


                                gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 >  2:4293808/1‑101 (MQ=255)


                                 ctctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccc                                                                <  1:316436/101‑1 (MQ=255)


                                 ctctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccc                                                                >  2:680612/1‑101 (MQ=255)


                                    tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             <  2:3060653/101‑1 (MQ=255)


                                     ttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCtt                                                            >  1:2399212/1‑101 (MQ=255)


                                     ttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCtt                                                            <  2:4437124/101‑1 (MQ=255)


                                     ttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCtc                                                            >  2:4772406/1‑100 (MQ=255)


                                      tGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTc                                                           <  1:4676346/101‑1 (MQ=255)


                                      tGGCAATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTc                                                           >  2:4273291/1‑101 (MQ=255)


                                       ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          >  2:1004873/1‑101 (MQ=255)


                                        gCTATCCCGGCAGCCCGACCCAGGGGGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                         <  1:817655/101‑1 (MQ=255)


                                        gCTATCACGCCAGCCCGACCCAGGGCGAGGTCGCCCTCGGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                         <  1:1613619/101‑1 (MQ=255)


                                        gCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                         <  2:4062811/101‑1 (MQ=255)


                                          tATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCa                                                       >  2:3405913/1‑101 (MQ=255)


                                           aTCACGACAGCCCGCCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  2:575383/101‑1 (MQ=255)


                                           aTCACGACAGCCCGCCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  1:3618818/101‑1 (MQ=255)


                                           aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  1:624949/101‑1 (MQ=255)


                                           aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      >  1:369411/1‑101 (MQ=255)


                                             cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    >  1:4500472/1‑101 (MQ=255)


                                              aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  1:3520593/101‑1 (MQ=255)


                                              aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   >  1:1327430/1‑101 (MQ=255)


                                               cGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAgg                                                  <  1:209244/101‑1 (MQ=255)


                                                  cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCt                                               <  2:2434595/101‑1 (MQ=255)


                                                    gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             >  1:4950878/1‑101 (MQ=255)


                                                        gccccAGGGCGAGGTCGCCCTCGGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         <  1:3102149/99‑1 (MQ=255)


                                                        gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         <  2:1805987/101‑1 (MQ=255)


                                                        gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         >  2:3169739/1‑101 (MQ=255)


                                                         aCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGa                                        >  1:2902505/1‑101 (MQ=255)


                                                           ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      <  1:2038138/101‑1 (MQ=255)


                                                            cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     <  2:845201/101‑1 (MQ=255)


                                                             aGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAc                                    >  2:3780461/1‑101 (MQ=255)


                                                             aGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGCCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAc                                    <  2:344774/101‑1 (MQ=255)


                                                              gggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcc                                   <  2:1582680/101‑1 (MQ=255)


                                                               ggCGAGGTCCCCCTCGGGGCCCGGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  <  2:1404926/101‑1 (MQ=255)


                                                                gCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcccc                                 >  1:1450407/1‑101 (MQ=255)


                                                                gCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcccc                                 >  1:3990129/1‑101 (MQ=255)


                                                                ctgAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCCGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcccc                                 <  1:4274044/99‑1 (MQ=255)


                                                                 cGAGGTTGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCTGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAGGGCCTGATCCGATCACCCCt                                >  1:1206444/1‑101 (MQ=255)


                                                                 cGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                                >  2:3243809/1‑101 (MQ=255)


                                                                 cGAGGTCGCCCGCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                                <  2:4058701/101‑1 (MQ=255)


                                                                 cGAGGTCCCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                                <  2:274549/101‑1 (MQ=255)


                                                                  gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               <  1:740113/101‑1 (MQ=255)


                                                                   aGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTc                              >  2:2108204/1‑101 (MQ=255)


                                                                    ggTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             >  1:3397823/1‑101 (MQ=255)


                                                                    ggTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  2:3876399/101‑1 (MQ=255)


                                                                     gTCGCCCTCTGGGCCGCGTCCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCg                            <  2:4952822/101‑1 (MQ=255)


                                                                        gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcac                         >  1:2492011/1‑101 (MQ=255)


                                                                        gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcac                         <  1:1191487/101‑1 (MQ=255)


                                                                         cccTCTGGGCCGCGTTTCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcaca                        <  1:2289537/101‑1 (MQ=255)


                                                                         cccTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcaca                        >  2:2323711/1‑101 (MQ=255)


                                                                           ctctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAg                      <  1:2418066/101‑1 (MQ=255)


                                                                            tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     >  1:4398501/1‑101 (MQ=255)


                                                                             ctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    >  2:3934749/1‑101 (MQ=255)


                                                                              tGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcgc                   <  1:2523991/101‑1 (MQ=255)


                                                                               gggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  >  2:2618649/1‑101 (MQ=255)


                                                                                   cgcgTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              >  2:4572369/1‑101 (MQ=255)


                                                                                     ggtcccTGGCCGTC.CCCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCgg            <  1:2210262/97‑1 (MQ=255)


                                                                                       ttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          <  1:3359232/101‑1 (MQ=255)


                                                                                       ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          >  1:3466611/1‑101 (MQ=255)


                                                                                        tCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcg         >  1:2695624/1‑101 (MQ=255)


                                                                                          cTGGCCGTG.ACCCTGCCGCTCTTCCCGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcc       <  2:1959360/101‑1 (MQ=255)


                                                                                              ccGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcct   >  2:3746931/1‑101 (MQ=255)


                                                                                               cGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCtt  <  1:2651301/101‑1 (MQ=255)


AATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258864‑3259058

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG