BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,560,922	+G	98.2%	coding (330/1134 nt)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,560,922	1	.→G	98.2%	3.4 ‑2.1 ‑0.2	220	coding (330/1134 nt)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand): new base (104/112): ref base (0/4): total (104/116)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.68e-01

Only 100 of 262 total aligned reads displayed.


CTGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCTCTTGCGACGCTGCCGATGG.C.G.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGA  >  NC_014034/3560821‑3561017


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  2:640178/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  1:1258399/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  2:4059011/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  2:3799536/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  1:1725540/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g                                                                                                  >  1:2101674/1‑101 (MQ=255)


  ggAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.g                                                                                                >  1:358388/1‑101 (MQ=255)


   gAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.gg                                                                                               >  2:1055958/1‑101 (MQ=255)


    aGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.ggg                                                                                              >  1:1022852/1‑101 (MQ=255)


       aaGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.gggggt                                                                                           >  2:4961028/1‑98 (MQ=255)


         gagGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.ggggattt                                                                                         <  1:3353491/101‑6 (MQ=255)


         gagGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.ggggatct                                                                                         <  2:6220/101‑6 (MQ=255)


         gagGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.g.ggggatct                                                                                         <  1:4371766/101‑6 (MQ=255)


          agGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTg                                                                                        >  2:2577305/1‑101 (MQ=255)


          agGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTg                                                                                        <  1:161638/101‑1 (MQ=255)


            gccgccGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGt                                                                                      <  2:1380320/101‑1 (MQ=255)


             ccgccgGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGtg                                                                                     <  1:4754584/101‑1 (MQ=255)


               gccgGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGtgct                                                                                   >  1:3037142/1‑101 (MQ=255)


                  ggTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCa                                                                                >  2:3017742/1‑101 (MQ=255)


                    ttttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATg                                                                              <  1:3799821/101‑1 (MQ=255)


                      ttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.G.GGGTATCTGGTGCTGCATGAc                                                                            <  2:4123686/101‑1 (MQ=255)


                         tCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTg                                                                         <  1:2571876/101‑1 (MQ=255)


                          cGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGc                                                                        >  1:83913/1‑101 (MQ=255)


                            gTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCTGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTg                                                                      <  1:4248204/101‑1 (MQ=255)


                            gTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTg                                                                      <  2:275842/101‑1 (MQ=255)


                            gTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTg                                                                      <  1:3507138/101‑1 (MQ=255)


                            gTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTg                                                                      <  1:3900418/101‑1 (MQ=255)


                            gTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTg                                                                      <  1:4129115/101‑1 (MQ=255)


                             tGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGa                                                                     <  2:3181779/101‑1 (MQ=255)


                               ccGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATc                                                                   <  1:1546892/101‑1 (MQ=255)


                                cGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATcc                                                                  >  2:2298554/1‑101 (MQ=255)


                                cGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATcc                                                                  <  2:3723636/101‑1 (MQ=255)


                                  aGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCAt                                                                >  2:3165748/1‑101 (MQ=255)


                                   ggCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATg                                                               <  2:3536286/101‑1 (MQ=255)


                                   ggCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATg                                                               <  2:4868832/101‑1 (MQ=255)


                                     ccTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGcc                                                             <  1:2656371/101‑1 (MQ=255)


                                      cTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCa                                                            <  2:2643147/101‑1 (MQ=255)


                                      cTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCa                                                            <  2:688839/101‑1 (MQ=255)


                                        gTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAgc                                                          >  2:3941356/1‑101 (MQ=255)


                                            tttCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAgcgcgc                                                      <  2:1627297/101‑1 (MQ=255)


                                              tCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGCCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCAt                                                    >  2:3094938/1‑101 (MQ=255)


                                               cacgggCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATg                                                   >  1:4717045/4‑101 (MQ=255)


                                               cATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATg                                                   <  1:2781301/101‑1 (MQ=255)


                                                aTGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGa                                                  >  1:1571616/1‑101 (MQ=255)


                                                     cggcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGGTG.G.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGc                                             <  2:4692219/101‑1 (MQ=255)


                                                     cggcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGc                                             >  1:1991966/1‑101 (MQ=255)


                                                     cggcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGc                                             >  1:1296695/1‑101 (MQ=255)


                                                      ggcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCt                                            <  2:2042349/101‑1 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           <  2:2825706/101‑1 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           <  1:4961358/101‑1 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           <  1:884199/101‑1 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           >  1:3270876/1‑101 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           <  2:1118816/101‑1 (MQ=255)


                                                        cggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGc                                          <  2:516559/101‑1 (MQ=255)


                                                         gggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCg                                         >  2:1683757/1‑101 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        <  2:1247792/101‑1 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        >  2:4389582/1‑101 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        <  1:3095176/101‑1 (MQ=255)


                                                           nntnNNNGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCggn                                       <  1:769943/99‑2 (MQ=255)


                                                           gTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGc                                       >  2:3531258/1‑101 (MQ=255)


                                                            tttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCt                                      <  1:2298718/101‑1 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTg                                     >  2:2019172/1‑101 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTg                                     >  2:1018823/1‑101 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.C.GTGGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTg                                     <  2:4978465/101‑1 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGACGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCACCGCGCATGCGGGGCTGCGGCTg                                     >  1:641267/1‑101 (MQ=255)


                                                               cAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCAGGAGGGGCTGCGCCCggg                                   >  2:3644482/1‑101 (MQ=255)


                                                                 ggCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCt                                 <  2:2725508/101‑1 (MQ=255)


                                                                  gCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTc                                >  2:3455390/1‑101 (MQ=255)


                                                                   ccAATCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCg                               >  1:4452596/1‑101 (MQ=255)


                                                                     aaTCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGc                             <  2:3270624/101‑1 (MQ=255)


                                                                     aaTCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGc                             <  2:3862514/101‑1 (MQ=255)


                                                                     aaTCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGc                             >  1:11777/1‑101 (MQ=255)


                                                                      aTCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGcc                            <  1:3034977/101‑1 (MQ=255)


                                                                      aTCAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGcc                            <  2:1943974/101‑1 (MQ=255)


                                                                        cAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCgg                          >  1:1107346/1‑100 (MQ=255)


                                                                        cAGGCGGTCT.TTGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGa                          <  1:1056068/101‑1 (MQ=255)


                                                                          ggCGGTCT.TTGCGGCGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAgg                        <  1:1969508/101‑1 (MQ=255)


                                                                                  cgatctaCGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAg                 <  2:2503499/95‑1 (MQ=255)


                                                                                   ttGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGAAGGGCTGATCCATGCCCGCGCGCATGAGGGGCTGCGGCTGGGCTCGTCCGAGGCACGCAGc                <  1:5002436/101‑1 (MQ=255)


                                                                                    tGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCt               >  1:293735/1‑101 (MQ=255)


                                                                                    tGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCt               >  1:619359/1‑101 (MQ=255)


                                                                                    tGCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCt               <  2:1786054/101‑1 (MQ=255)


                                                                                     gCGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCtt              >  1:4063556/1‑101 (MQ=255)


                                                                                      cGACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCttt             <  1:2673685/101‑1 (MQ=255)


                                                                                       gACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            <  1:4883551/101‑1 (MQ=255)


                                                                                       gACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            <  1:3614004/101‑1 (MQ=255)


                                                                                       gACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            <  1:3392755/101‑1 (MQ=255)


                                                                                       gACGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            <  2:1296574/101‑1 (MQ=255)


                                                                                        aCGCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAgagatcg           >  1:2503673/1‑95 (MQ=255)


                                                                                          gCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGcc         <  2:3359926/101‑1 (MQ=255)


                                                                                          gCTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAAGCAGGCAGCCTTAGcc         >  2:451092/1‑101 (MQ=255)


                                                                                           cTGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCca        <  1:2213461/101‑1 (MQ=255)


                                                                                            tGCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcac       >  1:724599/1‑101 (MQ=255)


                                                                                             gCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      <  1:3941648/101‑1 (MQ=255)


                                                                                             gCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      >  2:2367359/1‑101 (MQ=255)


                                                                                             gCCGATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      >  2:2893083/1‑101 (MQ=255)


                                                                                               cGATGG.CGG.GGG.TTCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAAt    >  1:2742761/1‑101 (MQ=255)


                                                                                                gATGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATg   <  1:3185987/101‑1 (MQ=255)


                                                                                                 aTGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGa  <  1:2518415/101‑1 (MQ=255)


                                                                                                 aTGG.CGG.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGa  <  2:1724383/101‑1 (MQ=255)


CTGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCTCTTGCGACGCTGCCGATGG.C.G.GGG.ATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGA  >  NC_014034/3560821‑3561017

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG