Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 774,643 +C 100% intergenic (‑97/+23) oppB1 ← / ← oppA1 oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034774,6431.→C100.0% 79.4 21intergenic (‑97/+23)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (17/4):  ref base (0/0):  total (17/4)

CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774585‑774728
                                                                    |                                                                                     
cGGGGGGGCGCCGGGCCGCAGGCCCGGCGCCCCCCCGCCCGGGGCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCcg                                                     >  1:4468860/1‑101 (MQ=255)
                  caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                   >  1:88244/9‑101 (MQ=255)
                  caggcccggcgcCCCCCCGCCCGCGTCGGACCGGCCCTGCC.GGCC.CGACCCCACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACggt                                   >  2:2452122/9‑99 (MQ=255)
                   aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGG.C.CGACCCTACCGGCTCTTATCCTGCGATCACTTCGCCCGCGACCCGACGGCCACGTTc                                  >  1:4561874/8‑101 (MQ=255)
                    ggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTcc                                 >  1:1432018/7‑101 (MQ=255)
                      cccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGGCGGCCACGTTCCAg                               >  2:4668931/5‑101 (MQ=255)
                         ggcgcCCCCCCGCCCGGGACGGACCGGCCCTGCC.CG.TCCGACCCTACGGTCTCTTATCCTGCGATCCCCTCGCCCGCGACCCGTCGGCCCCGTTCCAATAt                            >  2:2109406/2‑101 (MQ=255)
                                     cccgggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAg                >  2:2366646/11‑101 (MQ=255)
                                         ggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  2:3877344/7‑101 (MQ=255)
                                         ggtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  2:3596841/7‑101 (MQ=255)
                                          gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:235576/6‑101 (MQ=255)
                                          gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  2:3021290/6‑101 (MQ=255)
                                          gtcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt           >  1:3013283/6‑101 (MQ=255)
                                           tcggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc          >  2:3808140/5‑101 (MQ=255)
                                            cggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:3469827/4‑101 (MQ=255)
                                             ggaCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  2:3798703/3‑101 (MQ=255)
                                               aCCGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct      <  1:417746/101‑1 (MQ=255)
                                                ccGGCCCTGCC.GG.TCCGACCCTGCGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     <  1:2561524/101‑1 (MQ=255)
                                                ccGGCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc     <  1:1850282/101‑1 (MQ=255)
                                                  ggCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:3183964/1‑101 (MQ=255)
                                                  ggCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  1:2572980/1‑101 (MQ=255)
                                                  gccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   <  2:4468541/99‑1 (MQ=255)
                                                   gCCCTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCgcc  >  1:1245528/1‑99 (MQ=255)
                                                   ccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCgcc  <  2:1245407/100‑3 (MQ=255)
                                                   ccccTGCC.GG.TCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  2:4221264/100‑1 (MQ=255)
                                                                    |                                                                                     
CGGGGGGGCGCCGGGCCGCA......GCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GG.T.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774585‑774728

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG