Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 925,909 +C 93.2% intergenic (+162/+12) RCAP_rcc00838 → / ← glcB DnaJ domain‑containing protein/malate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034925,9091.→C93.2% 42.1 ‑2.7 ‑1.0236intergenic (+162/+12)RCAP_rcc00838/glcBDnaJ domain‑containing protein/malate synthase
Reads supporting (aligned to +/- strand):  new base (137/83):  ref base (6/10):  total (143/93)
Fisher's exact test strand distribution p-value = 6.41e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.72e-02

Only 100 of 260 total aligned reads displayed.

CCCCCCGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAAGACCA  >  NC_014034/925810‑926009
                                                                                                       |                                                                                                    
 cccccGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.cc                                                                                                    <  2:4886123/101‑1 (MQ=255)
    ccGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.ccccc                                                                                                 <  2:1695872/101‑1 (MQ=255)
    ccGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.ccccc                                                                                                 >  2:4495408/1‑101 (MQ=255)
     cGGGATATTTGGGGCAAGGTGAAA.GGAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.cccccc                                                                                                >  2:1891304/1‑101 (MQ=255)
     cGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.cccccc                                                                                                >  2:3590690/1‑101 (MQ=255)
       ggATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.cccccccc                                                                                              >  1:4779253/1‑100 (MQ=255)
        gATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTCCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.cccccccct                                                                                             <  1:3221808/101‑3 (MQ=255)
          tatTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCcttt                                                                                           <  1:723204/101‑2 (MQ=255)
          tatTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCcttt                                                                                           >  1:1088701/1‑100 (MQ=255)
           atTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctttc                                                                                          >  1:3078795/1‑99 (MQ=255)
           atTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctttc                                                                                          >  1:2048567/1‑99 (MQ=255)
            tttGGGGGAAGGGAAAACGCGGCCCGTTTTCCCTTTTCATTTTCCCCAAAATCC.CCGGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctttc                                                                                          <  2:1026647/101‑3 (MQ=2)
             ttGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCtt                                                                                         <  1:2817780/101‑1 (MQ=255)
              tGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTa                                                                                        >  1:3046969/1‑101 (MQ=255)
               ggggCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTAt                                                                                       <  1:4110284/101‑1 (MQ=255)
                 gggaaGGGGGAA.GCCGGCCCCTTTTCCCTTT.TCCTTGCCCCCAAAATACCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGc                                                                                     <  1:2009565/98‑1 (MQ=2)
                 gggaaGGGGAAA.GCGGGCCGCTTTGCCCTTTCATCTTGCCCAAAATAT.CCCGGGGGGAGTCCGCTTGGGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGc                                                                                     <  1:1389306/98‑1 (MQ=18)
                 ggCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGc                                                                                     <  2:2252978/101‑1 (MQ=255)
                 ggCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGc                                                                                     <  2:2803397/101‑1 (MQ=255)
                  gCACGGTGAAA.GCCGGCNGCTTTGCCCTTTCATCTTGCCACAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCt                                                                                    <  2:760169/101‑1 (MQ=18)
                  gCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTTTGCt                                                                                    >  2:1090761/1‑101 (MQ=255)
                   cAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.GGCCCCCCCCTTTCTTATGCtt                                                                                   >  2:2913553/1‑101 (MQ=255)
                   cAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATTCtt                                                                                   >  1:4408473/1‑101 (MQ=255)
                    aaGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCttt                                                                                  >  1:4162096/1‑101 (MQ=255)
                        tGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcg                                                                              >  2:3090379/1‑101 (MQ=255)
                         gAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcgg                                                                             >  2:3457499/1‑101 (MQ=255)
                         gAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcgg                                                                             >  2:1472707/1‑101 (MQ=255)
                          aaa.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcggc                                                                            >  1:3170797/1‑101 (MQ=255)
                            a.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCt                                                                          >  2:4757655/1‑101 (MQ=255)
                            a.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCt                                                                          >  2:4911368/1‑101 (MQ=255)
                            a.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCt                                                                          <  1:3814445/101‑1 (MQ=255)
                              gcgggCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCtt                                                                         <  2:4623928/98‑1 (MQ=255)
                              gCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCtt                                                                         <  2:1863861/101‑1 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                        <  2:4111072/101‑1 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                        >  2:5038201/1‑101 (MQ=255)
                               cAGGCCCCTTTCCCCTTTCATCTGGCCCCAAATAC.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                        <  1:101443/101‑1 (MQ=18)
                                 ggCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAc                                                                      <  2:3836333/101‑1 (MQ=255)
                                 ggCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAc                                                                      >  2:2034456/1‑101 (MQ=255)
                                  gCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAcc                                                                     >  2:1620435/1‑101 (MQ=255)
                                  gCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAcc                                                                     <  1:1290892/101‑1 (MQ=255)
                                   ccgtttcccccTTTCATCTTCCCCCAAATAC.CCCGGGGGGAGCCCGCTGGCGGGGGGGGGGCAGCCC.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCt                                                                    <  2:2927486/93‑1 (MQ=255)
                                    cGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                                   <  2:3014859/101‑1 (MQ=255)
                                      cTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcg                                                                 <  2:2837132/101‑1 (MQ=255)
                                       tttGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcgc                                                                >  1:1707919/1‑101 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGAGGAT.CGCCCCCCCCTGTCTTATCCTTTGGCGGCCTTCAACTGCGcc                                                               >  2:1991837/1‑101 (MQ=18)
                                          gCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCg                                                             >  2:3909471/1‑101 (MQ=255)
                                          gCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCg                                                             <  2:2401144/101‑1 (MQ=255)
                                              tttCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgcgc                                                         <  2:3666707/101‑1 (MQ=255)
                                               ttCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCt                                                        >  2:4979921/1‑101 (MQ=255)
                                               ttCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCt                                                        <  1:2405232/101‑1 (MQ=255)
                                                tCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCCCCTGCCCCCGGCGCTg                                                       >  1:1808822/1‑101 (MQ=21)
                                                tCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTg                                                       >  1:557005/1‑101 (MQ=255)
                                                tCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACATGCGCCCGGCGCTg                                                       <  1:1253035/101‑1 (MQ=255)
                                                   tCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTg                                                    >  1:3338087/1‑101 (MQ=255)
                                                    ctgcccccAAATAT.CCGGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgc                                                   <  2:3690006/97‑1 (MQ=25)
                                                      tGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcag                                                 <  1:233999/101‑1 (MQ=255)
                                                      tGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcag                                                 >  2:3251665/1‑101 (MQ=255)
                                                          ccAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTTCGCCCGGCGCTGGTGCAGCAcc                                             >  2:850113/1‑101 (MQ=255)
                                                          ccAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAcc                                             <  2:1290463/101‑1 (MQ=255)
                                                          ccAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAcc                                             <  2:652247/101‑1 (MQ=255)
                                                           cAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCg                                            <  1:3748314/101‑1 (MQ=255)
                                                            aaaTAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTTCAGCACCgg                                           >  1:4951376/1‑101 (MQ=255)
                                                            aaaTAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCgg                                           >  1:957585/1‑101 (MQ=255)
                                                            aaaTAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCgg                                           >  1:851433/1‑101 (MQ=255)
                                                            aaaTAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCgg                                           >  2:4232974/1‑101 (MQ=255)
                                                            aaaTAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGGGCAGCACCgg                                           >  1:893640/1‑101 (MQ=255)
                                                             aaTAT.CGCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGCGCAGCACCGGc                                          >  2:2693642/1‑101 (MQ=25)
                                                              atat.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCCTCACCTGCGCCCGGCGCTGGTGCAGCACCGGcc                                         >  1:4350406/1‑100 (MQ=255)
                                                              atat.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTCTTATCTTTTGGCGGCCTTCAACTGCCCCCGGCGCTGGTGCACCCCCGGCt                                         >  1:2289591/1‑101 (MQ=18)
                                                               tat.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTc                                        <  2:4448600/101‑1 (MQ=255)
                                                               tat.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGGGCAGCACCGGCTc                                        >  1:463830/1‑101 (MQ=255)
                                                                    ccGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGta                                    >  2:3417139/1‑101 (MQ=255)
                                                                     cGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTccggaa                                   >  2:3042335/1‑96 (MQ=255)
                                                                     cGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                                   <  1:2680913/101‑1 (MQ=255)
                                                                     cGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                                   <  2:4017290/101‑1 (MQ=255)
                                                                     cGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                                   <  2:714007/101‑1 (MQ=255)
                                                                      ggggggAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                                  >  1:1431037/1‑101 (MQ=255)
                                                                      ggggggAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGgata                                  >  1:2723746/1‑101 (MQ=25)
                                                                      cgatctaGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                                  <  1:4041886/95‑1 (MQ=37)
                                                                       gggggAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                                 <  2:1235203/101‑1 (MQ=255)
                                                                       gggggAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                                 <  2:1759226/101‑1 (MQ=255)
                                                                        ggggAGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGc                                <  1:1560520/101‑1 (MQ=255)
                                                                            aGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCCCCGGCTCGGTATAGCCCgg                            >  1:4466061/1‑100 (MQ=255)
                                                                            aGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCgc                            >  1:4917344/1‑100 (MQ=255)
                                                                            aGTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGGGCAGCACCGGCTCGGTATAGCCCGa                            >  1:690264/1‑101 (MQ=255)
                                                                             gTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAg                           >  1:376302/1‑101 (MQ=255)
                                                                               ccGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGa                         >  1:393726/1‑101 (MQ=255)
                                                                               ccGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGa                         >  1:289670/1‑101 (MQ=255)
                                                                                 ccTTGGGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACt                       <  2:1510794/100‑1 (MQ=255)
                                                                                   ttGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTgg                     >  1:1837372/1‑101 (MQ=255)
                                                                                       ggACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATcc                 <  1:3383300/101‑1 (MQ=255)
                                                                                       ggACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATcc                 <  1:1066372/101‑1 (MQ=255)
                                                                                        gACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCg                >  1:1517496/1‑101 (MQ=255)
                                                                                          cGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg              >  1:3291549/1‑101 (MQ=255)
                                                                                          cGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg              >  2:31692/1‑101 (MQ=255)
                                                                                            gggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCg            >  2:4827932/1‑101 (MQ=255)
                                                                                            gggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCg            >  2:1425058/1‑101 (MQ=255)
                                                                                            gggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCg            >  2:2553908/1‑101 (MQ=255)
                                                                                               ggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACCGGATCCGGGCGGgg         >  1:3661069/1‑101 (MQ=255)
                                                                                                 cAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGaa       <  1:2113580/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
CCCCCCGGGATATTTGGGGCAAGGTGAAA.GCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAAGACCA  >  NC_014034/925810‑926009

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG