Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,265	+C	100%	intergenic (+145/+114)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,265	1	.→C	100.0%	97.2	27	intergenic (+145/+114)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/11):  ref base (0/0):  total (16/11)

GACCCGCCGTCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACA  >  NC_014034/974190‑974342                                                                             |                                                                                   cggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCtt                                                            <  1:1352596/92‑1 (MQ=25) cggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCtt                                                            <  2:3266107/92‑1 (MQ=25)  gggggnnttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTg                                                           <  1:2229558/93‑1 (MQ=37)    ggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCg                                                         <  1:1222570/95‑1 (MQ=37)     gggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGa                                                        >  1:610382/6‑101 (MQ=37)      ggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGAc                                                       <  2:4701755/97‑1 (MQ=255)      ggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGAc                                                       >  2:1942312/5‑101 (MQ=255)        cttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTTCGGCaa                                                     >  2:4394507/3‑101 (MQ=38)        cttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaa                                                     <  1:2604560/99‑1 (MQ=255)        cttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaa                                                     >  2:3742387/3‑101 (MQ=255)        cttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGGTTTCTCCTTGCGACaa                                                     >  2:4676226/3‑101 (MQ=37)         ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaaa                                                    >  1:1828298/2‑101 (MQ=255)         ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaaa                                                    >  2:4724014/2‑101 (MQ=255)         ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaaa                                                    <  2:2422167/100‑1 (MQ=255)           cGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGCCGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaaaga                                                  >  1:1200342/1‑99 (MQ=255)            gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTCCGACAAaaat                                                 >  2:941001/1‑101 (MQ=38)              cccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGGTTTGGACTTTCGACAAATATcc                                               >  1:3068661/1‑101 (MQ=37)               ccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATcc.c                                             <  1:1942449/101‑1 (MQ=255)                cccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCCCCTTGCGACAAATATCC.C.g                                           >  1:565942/1‑101 (MQ=255)                  cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGCCAAATATCC.C.cgg                                         >  2:4582272/1‑101 (MQ=38)                  cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGACAAATATCC.C.ggg                                         >  2:1134664/1‑101 (MQ=255)                  cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.ggg                                         >  2:453677/1‑101 (MQ=255)                  cGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.ggg                                         >  1:2507632/1‑101 (MQ=255)                   ggACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGGCAAATATCC.C.gggg                                        >  2:575230/1‑101 (MQ=255)                     aCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGACAAATATTC.C.gggggg                                      >  2:4505351/1‑99 (MQ=38)                     aCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.gggggg                                      >  1:3158369/1‑99 (MQ=255)                      ccccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.gggggtc                                     <  1:2331296/101‑4 (MQ=255)                       cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCCTTCGACCAAAATCC.C.cgggggcc                                    >  1:2720924/1‑97 (MQ=25)                        ccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.gggggtccg                                   <  2:745163/101‑6 (MQ=255)                                                         ggcgggATCGGGCAGGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCGG.GGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGAc   <  2:1200225/101‑1 (MQ=2)                                                          gcgggATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.CGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACa  <  2:610303/101‑1 (MQ=14)                                                                             |                                                                                   GACCCGCCGTCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCC.C.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACA  >  NC_014034/974190‑974342 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG