New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	= 1224266	258 (1.170)	48 (0.220)	13/176	19.6	0.17	intergenic (‑138/+31)	hisF/hisA	imidazole glycerol phosphate synthase subunit HisF/1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino] imidazole‑4‑carboxamide isomerase
	?	NC_014034	3011299 =	213 (0.870)					intergenic (+98/+44)	purK/RCAP_rcc02822	phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Rejected: Position hash score below cutoff.

GGCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224157‑1224266 ....................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398                                                                                                                                                                                                                              GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     >  2:2896327/1‑101 (MQ=1)            GCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATCTCCCGGGGGGAG                                                                                                    <  2:1203616/101‑1 (MQ=1)            GCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGAG                                                                                                    <  1:3925733/101‑1 (MQ=1)            GCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGAG                                                                                                    <  2:262216/101‑1 (MQ=1)                                                                                  tTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCC                              >  1:3866678/2‑101 (MQ=255)                                                                                  tTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCC                              >  1:1109185/2‑101 (MQ=255)                                                                                  tTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCC                              >  1:2368433/2‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:2759355/1‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:4525528/1‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:4267003/1‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:1234510/1‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:4524825/1‑101 (MQ=255)                                                                                    TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:4757462/1‑101 (MQ=9)                                                                                      accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCTTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          <  1:1078483/93‑1 (MQ=255)                                                                                      accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCACCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          >  1:1085608/9‑101 (MQ=255)                                                                                       ccttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCC                         <  1:3427837/94‑1 (MQ=2)                                                                                        cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        <  1:2950683/95‑1 (MQ=2)                                                                                        cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        >  1:4848156/7‑101 (MQ=2)                                                                                        cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        >  1:3242850/7‑101 (MQ=2)                                                                                         ttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       <  2:2226729/96‑1 (MQ=11)                                                                                         ttgggACAAATACCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       <  1:2908845/96‑1 (MQ=2)                                                                                         tggggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       <  1:714256/96‑1 (MQ=11)                                                                                          tgggaaAAATATCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  1:1746090/95‑1 (MQ=0)                                                                                          tgggaaAAATACCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  1:3031095/95‑1 (MQ=0)                                                                                          tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCCAGGCCGCGCCCCA                      >  2:1083453/5‑101 (MQ=2)                                                                                          tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCCAGGCCGCGCCCCA                      >  2:992246/5‑101 (MQ=2)                                                                                          tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCCCTGCAAGGCCGCGCCCCc                      >  1:4798571/5‑100 (MQ=2)                                                                                          tgggACAAATACCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  2:3469655/97‑1 (MQ=2)                                                                                           gggaaAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     <  1:1769037/96‑1 (MQ=1)                                                                                           gggaaAAATACCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     <  1:2612321/96‑1 (MQ=0)                                                                                           gggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAc                     >  1:2042248/4‑100 (MQ=11)                                                                                           gggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     <  2:4599054/98‑1 (MQ=11)                                                                                           gggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     >  2:3348957/4‑101 (MQ=11)                                                                                            ggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAcc                    >  2:3844703/3‑99 (MQ=11)                                                                                             gACAAATATCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCC                   <  2:2560429/100‑1 (MQ=11)                                                                                              ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCC                  >  1:3037980/1‑101 (MQ=11)                                                                                              ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCC                  >  2:4313707/1‑101 (MQ=11)                                                                                              ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCC                  >  2:3010218/1‑101 (MQ=11)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGG                >  2:52471/1‑101 (MQ=1)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGG                >  1:4593252/1‑101 (MQ=1)                                                                                                 AATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGC               >  1:2810061/1‑101 (MQ=1)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGGCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  1:410065/1‑101 (MQ=1)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  2:2641778/1‑101 (MQ=1)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  2:1633374/1‑101 (MQ=1)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  1:2594541/1‑101 (MQ=1)                                                                                                   TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCCGCCCGGCAC             >  1:159951/1‑101 (MQ=1)                                                                                                   TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  1:742075/1‑101 (MQ=1)                                                                                                   TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  2:4348559/1‑101 (MQ=1)                                                                                                   TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  1:3341971/1‑101 (MQ=1)                                                                                                    ATCCGGGGGGGAAACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            <  2:2163404/101‑1 (MQ=0)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCCCC            >  1:1010851/1‑101 (MQ=1)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            >  1:916741/1‑101 (MQ=1)                                                                                                                                                                                                                    GGCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224157‑1224266 ....................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG