Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034243,6340G→C 1.3% 0.9 ‑2.0 ‑0.3298intergenic (+63/+57)mogA/ubiGmolybdopterin biosynthesis mog protein/3‑demethylubiquinone‑9 3‑O‑methyltransferase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (169/125):  total (173/125)
Fisher's exact test strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.09e-01

Only 100 of 326 total aligned reads displayed.

GGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGATCGC  >  NC_014034/243535‑243734
                                                                                                        |                                                                                                    
     aaaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTg                                                                                                <  1:2528061/101‑1 (MQ=255)
      aaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCAACCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGt                                                                                               >  2:3627691/1‑101 (MQ=255)
       aTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCGCCCCCGGGATAGTTGAGGCAAGAT.AAAGGAGCCTGtt                                                                                              <  2:158720/101‑1 (MQ=255)
       aTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtt                                                                                              <  1:1221650/101‑1 (MQ=255)
         cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCGCTC.TGGC..TTCGCC.ATTCACACCCCGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                                                            >  1:4322195/1‑101 (MQ=255)
         cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                                                            <  1:510449/101‑1 (MQ=255)
         cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                                                            <  2:1289913/101‑1 (MQ=255)
         cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                                                            <  1:1057115/101‑1 (MQ=255)
            ggcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATc                                                                                         >  1:1428825/1‑101 (MQ=255)
            ggcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATc                                                                                         <  2:4270120/101‑1 (MQ=255)
             gcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCt                                                                                        <  1:4144229/101‑1 (MQ=255)
             gcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCt                                                                                        >  2:3956992/1‑101 (MQ=255)
               gTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTg                                                                                      <  2:674911/101‑1 (MQ=255)
               gTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTg                                                                                      >  2:2891589/1‑101 (MQ=255)
                  tCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCt                                                                                   <  2:472282/101‑1 (MQ=255)
                   cGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.AGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTc                                                                                  <  1:3511076/101‑1 (MQ=255)
                    gCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCa                                                                                 <  1:3295137/101‑1 (MQ=255)
                    gCCCGAAGACGAAGTAAAAGGGGGCGCTCCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCa                                                                                 <  1:3847225/101‑1 (MQ=255)
                     cccGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCaa                                                                                <  2:3206193/101‑1 (MQ=255)
                      ccGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCaaa                                                                               <  1:240511/101‑1 (MQ=255)
                      ccGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCaaa                                                                               <  1:1518315/101‑1 (MQ=255)
                      ccGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCaaa                                                                               >  1:4012101/1‑101 (MQ=255)
                        gAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATg                                                                             >  2:3575222/1‑101 (MQ=255)
                         aaGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGt                                                                            <  1:896737/101‑1 (MQ=255)
                         aaGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGt                                                                            >  1:4893149/1‑101 (MQ=255)
                           gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTcc                                                                          >  1:2578809/1‑101 (MQ=255)
                           gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTcc                                                                          <  2:3486654/101‑1 (MQ=255)
                           gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTcc                                                                          >  2:562712/1‑101 (MQ=255)
                           gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTcc                                                                          <  1:1914412/101‑1 (MQ=255)
                             cGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCg                                                                        <  2:3297187/101‑1 (MQ=255)
                              gAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGCGATATTTGAGGCAAGAT.AAAGGATCCTGTTTTATCTTGCCTCAAATGTCCCgg                                                                       >  2:853031/1‑101 (MQ=255)
                               aaGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCggg                                                                      >  1:3810552/1‑101 (MQ=255)
                                 gTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCggggg                                                                    <  2:4867084/101‑1 (MQ=255)
                                  gggcaGGGGGCGCTGCCCCCTCTTGGCCTTTGGCCAATTCACCCCTGGGATATTTCGGGCAAGATGAAATCATGCCCCTTCATCTTGCCGCAAATATCCCg                                                                        >  2:2059290/5‑101 (MQ=0)
                                  gggcaGGGGGCGCTGCCCCCTCTTGGCCTTTGGCCAATTCACCCCTGGGATATTTCGGGCAAGATGAAATCATGCCCCTTCATCTTGCCGCAAATATCCCg                                                                        >  2:284437/5‑101 (MQ=0)
                                   aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                                                  <  2:4977770/101‑1 (MQ=255)
                                   aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                                                  >  2:1407155/1‑101 (MQ=255)
                                   aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                                                  >  2:2735687/1‑101 (MQ=255)
                                   aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                                                  <  2:4377347/101‑1 (MQ=255)
                                   aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                                                  <  1:3398034/101‑1 (MQ=255)
                                    aaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCt                                                                 >  2:1641171/1‑101 (MQ=255)
                                       gggggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTggg                                                              >  1:1757879/1‑101 (MQ=255)
                                       gggggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTggg                                                              >  1:1192276/1‑101 (MQ=255)
                                         gggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTggggg                                                            <  2:3374492/101‑1 (MQ=255)
                                          ggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATTTCCCGGGGGCCTGGGGGc                                                           <  2:1761154/101‑1 (MQ=255)
                                           gcgcTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCa                                                          <  1:4604751/101‑1 (MQ=255)
                                            cgcTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAg                                                         >  2:2354908/1‑101 (MQ=255)
                                             gcTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAgc                                                        <  1:436361/101‑1 (MQ=255)
                                              cTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAgcg                                                       >  1:3900464/1‑101 (MQ=255)
                                                 cccccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGccc                                                    >  2:3842010/1‑101 (MQ=255)
                                                  ccccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGcccc                                                   <  1:4215660/101‑1 (MQ=255)
                                                    ccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCa                                                 >  1:4054247/1‑101 (MQ=255)
                                                       c.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGcc                                              >  2:2288448/1‑101 (MQ=255)
                                                         cggC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCt                                             <  2:4953482/100‑1 (MQ=255)
                                                          ggC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCtt                                            >  2:737650/1‑101 (MQ=255)
                                                          ggC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCtt                                            <  2:2435210/101‑1 (MQ=255)
                                                          ggC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCtt                                            >  1:2083339/1‑101 (MQ=255)
                                                           gC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTa                                           <  2:4121127/101‑1 (MQ=255)
                                                            c..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAg                                          >  2:4349370/1‑101 (MQ=255)
                                                            c..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAg                                          >  2:2175012/1‑101 (MQ=255)
                                                                 cGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGt                                       >  1:221874/1‑101 (MQ=255)
                                                                  gCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGtt                                      >  1:2449926/1‑101 (MQ=255)
                                                                  gCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGtt                                      >  1:1672559/1‑101 (MQ=255)
                                                                    c.ATTCCCCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTc                                    <  2:1475335/101‑1 (MQ=255)
                                                                      aTTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCa                                   >  1:3333716/1‑101 (MQ=255)
                                                                       ttCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCAc                                  <  2:3748741/101‑1 (MQ=255)
                                                                         cACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGc                                >  1:4262442/1‑101 (MQ=255)
                                                                            ccccGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAgg                             <  2:3895018/101‑1 (MQ=255)
                                                                             cccGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAggc                            <  1:5007767/101‑1 (MQ=255)
                                                                              ccGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAggcg                           >  2:3106548/1‑101 (MQ=255)
                                                                                    tatTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGgcggcggcg                     >  2:293074/1‑101 (MQ=255)
                                                                                      tttGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTa                   <  1:2914116/101‑1 (MQ=255)
                                                                                       ttGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGGGGCGtgg                  >  1:4752664/1‑99 (MQ=255)
                                                                                        tGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGt                 <  2:3241912/101‑1 (MQ=255)
                                                                                        tGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGt                 >  2:1681203/1‑101 (MQ=255)
                                                                                        tGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGt                 >  2:4757719/1‑101 (MQ=255)
                                                                                         gAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGtt                >  1:543762/1‑101 (MQ=255)
                                                                                         gAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTACtt                <  1:2891802/101‑1 (MQ=255)
                                                                                          aGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTc               <  1:3052214/101‑1 (MQ=255)
                                                                                          aGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTc               <  2:1192159/101‑1 (MQ=255)
                                                                                          aGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTc               >  1:4950209/1‑101 (MQ=255)
                                                                                          aGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCCTAGCGTTTCACGCagg.ggcggccggagttc              >  1:1023533/1‑93 (MQ=37)
                                                                                            gaaaGAG.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCAc             <  1:3627962/99‑1 (MQ=255)
                                                                                             cAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACg            >  2:4908677/1‑101 (MQ=255)
                                                                                             cAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACg            >  1:365907/1‑101 (MQ=255)
                                                                                               aGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCt          >  2:1065787/1‑101 (MQ=255)
                                                                                                gAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTg         <  1:3113135/101‑1 (MQ=255)
                                                                                                 aT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCGGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTga        <  2:4454136/101‑1 (MQ=255)
                                                                                                 aT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTga        >  1:3567301/1‑101 (MQ=255)
                                                                                                 aT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTga        >  1:1898185/1‑101 (MQ=255)
                                                                                                  t.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCGGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgag       <  2:2568209/101‑1 (MQ=255)
                                                                                                  t.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgag       >  2:4024868/1‑101 (MQ=255)
                                                                                                  t.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgag       <  2:2626338/101‑1 (MQ=255)
                                                                                                  t.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgag       >  1:1269810/1‑101 (MQ=255)
                                                                                                    aaaGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgaga      <  1:4440702/101‑1 (MQ=255)
                                                                                                    aaaGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTgaga      >  2:3660933/1‑101 (MQ=255)
                                                                                                     aaGTAGCTTGTTTTATCTGTGCGGAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGAt     <  1:2268696/101‑1 (MQ=37)
                                                                                                     aaGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGAt     >  1:757303/1‑101 (MQ=255)
                                                                                                      aGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGATc    <  2:2526476/101‑1 (MQ=255)
                                                                                                      aGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGATc    >  2:4784213/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
GGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTCACGCAGGCGGCGGCGTAGTTCACGCTGAGATCGC  >  NC_014034/243535‑243734

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG