BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,475	T→G	18.4%	intergenic (+287/‑644)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,475	0	T→G	18.4%	142.3 ‑1.5 ‑0.0	281	intergenic (+287/‑644)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (20/32): ref base (111/118): total (131/150)
Fisher's exact test strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 294 total aligned reads displayed.


CGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCC  >  NC_014034/1395376‑1395575


 gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                                                                    <  2:3032145/101‑1 (MQ=255)


     gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                                                                >  1:3857868/1‑101 (MQ=255)


      ggCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                                                               <  2:3270904/101‑1 (MQ=255)


         gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                                                                            >  2:2527687/1‑101 (MQ=255)


          tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                                                                           <  2:537328/101‑1 (MQ=255)


           ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                                                                          >  2:4778598/1‑101 (MQ=255)


            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                         <  1:2928791/101‑1 (MQ=255)


             aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGAGCGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTTGACAgg                                                                                        <  2:866079/101‑1 (MQ=255)


                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                     <  1:2880644/101‑1 (MQ=255)


                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                     >  1:5048942/1‑101 (MQ=255)


                 aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                                                                    >  2:3597497/1‑101 (MQ=255)


                 aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                                                                    >  1:2146742/1‑101 (MQ=255)


                  aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                                                                   <  2:3733854/101‑1 (MQ=255)


                    cTGCACCCGCTGGCCCCGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                                                                 >  1:149226/1‑101 (MQ=255)


                     tGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                <  2:4243897/101‑1 (MQ=255)


                      gcccccGCGGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                               <  2:639599/98‑1 (MQ=255)


                      gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaagg                                                                               >  1:679051/1‑99 (MQ=255)


                          ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                           >  1:1097574/1‑101 (MQ=255)


                            gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                         >  2:1677873/1‑101 (MQ=255)


                              gtgcacTGCCGGTGATCGGTGATCCGCCGCCGCCCCCCCCGCGGGGGGGTGGATCGAAAGATCGCCGCTTTGGCGGTGGGCAGGCTGGTAACGTGTCGCTc                                                                       <  2:1588092/96‑1 (MQ=255)


                               ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                      >  1:3254709/1‑101 (MQ=255)


                               ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGATGGTAACGTGTCGCTcc                                                                      >  2:719928/1‑101 (MQ=255)


                                gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                     <  1:2291375/101‑1 (MQ=255)


                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                    >  2:4610795/1‑101 (MQ=255)


                                 cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                    <  2:444843/101‑1 (MQ=255)


                                   cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                  <  1:3042657/101‑1 (MQ=255)


                                     gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                <  2:3955259/101‑1 (MQ=255)


                                       cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                              >  2:1387923/1‑101 (MQ=255)


                                         gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                            >  1:4410603/1‑101 (MQ=255)


                                          tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                           >  1:2479874/1‑101 (MQ=255)


                                           gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                          >  2:1018746/1‑101 (MQ=255)


                                              cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                       >  1:581195/1‑101 (MQ=255)


                                               ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                      >  1:2653659/1‑101 (MQ=255)


                                                gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                     <  1:3499439/101‑1 (MQ=255)


                                                gctgggcgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:2367564/92‑1 (MQ=0)


                                                 tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGtcgca                                                    >  1:1393888/1‑97 (MQ=255)


                                                 tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:2812710/101‑1 (MQ=255)


                                                 ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:4939415/93‑1 (MQ=0)


                                                 ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:4009993/93‑1 (MQ=0)


                                                 ctggacgtggTCGCTCCCACCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  2:3685569/9‑101 (MQ=0)


                                                  gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  1:3378767/1‑101 (MQ=255)


                                                   ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 >  1:3134655/7‑101 (MQ=2)


                                                   atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  1:4755427/101‑1 (MQ=255)


                                                   accgggggTCGCCCCCCCCGCGGGGGGGGGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCg                                                  <  2:1257018/95‑1 (MQ=38)


                                                    tcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  1:747863/101‑1 (MQ=255)


                                                      cggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:2283641/98‑1 (MQ=2)


                                                       gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGCGTCGCTCCCCCCGCCGGGGCGTGGATCGcaaaa                                             >  1:1992925/3‑99 (MQ=2)


                                                       gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                             <  1:2754232/99‑1 (MQ=2)


                                                       gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                             >  1:493847/3‑101 (MQ=2)


                                                       gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:1736651/101‑1 (MQ=255)


                                                       gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  2:4873420/101‑1 (MQ=255)


                                                        cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                             >  1:4576618/1‑101 (MQ=255)


                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                           >  2:2210255/1‑99 (MQ=2)


                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                            <  1:2059217/101‑1 (MQ=255)


                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                            <  1:1218691/101‑1 (MQ=255)


                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAt                                            >  2:2088410/1‑101 (MQ=255)


                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                           <  2:3659068/101‑1 (MQ=255)


                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                           <  1:164082/101‑1 (MQ=255)


                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                          <  1:4973501/101‑1 (MQ=255)


                                                            cccaccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                         <  2:4154969/97‑1 (MQ=255)


                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                        >  1:392142/1‑99 (MQ=2)


                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                        >  1:2560976/1‑99 (MQ=2)


                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                         >  2:4387371/1‑101 (MQ=255)


                                                             ccTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                       <  2:3503762/100‑4 (MQ=2)


                                                              ccccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                      <  2:4110621/99‑5 (MQ=0)


                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                      <  2:4340145/101‑5 (MQ=2)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                     >  1:3411170/1‑96 (MQ=2)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                      <  1:360359/101‑1 (MQ=255)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                      <  2:3428540/101‑1 (MQ=255)


                                                                ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                    <  2:322082/101‑7 (MQ=0)


                                                                  ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                   <  1:4796121/101‑1 (MQ=255)


                                                                    ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                 <  2:67312/101‑1 (MQ=255)


                                                                     cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                                <  2:1569565/101‑1 (MQ=255)


                                                                      gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCg                               >  2:419310/1‑101 (MQ=255)


                                                                      gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATCTCGGTGCCGTCg                               >  2:1506879/1‑101 (MQ=255)


                                                                        gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                             <  2:951424/101‑1 (MQ=255)


                                                                        gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                             >  2:1074800/1‑101 (MQ=255)


                                                                         ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                            >  2:3117919/1‑101 (MQ=255)


                                                                         ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                            >  1:222972/1‑101 (MQ=255)


                                                                         ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                            <  2:1107056/101‑1 (MQ=255)


                                                                            gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAATATCTCGGTGCCGTCGCTCGTc                         <  1:3727499/101‑1 (MQ=255)


                                                                             cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                        <  2:247596/101‑1 (MQ=255)


                                                                             cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                        <  2:3931898/101‑1 (MQ=255)


                                                                              gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                       <  2:1526942/101‑1 (MQ=255)


                                                                                ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                     >  2:2635514/1‑101 (MQ=255)


                                                                                    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                 >  2:1941249/1‑101 (MQ=255)


                                                                                    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                 >  2:3641671/1‑101 (MQ=255)


                                                                                    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCCGc                 >  2:2974136/1‑101 (MQ=255)


                                                                                     gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                >  2:2132770/1‑101 (MQ=255)


                                                                                          aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc           <  1:4107146/101‑1 (MQ=255)


                                                                                          aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGCCCAGCCGGCCTGCtc           >  1:1076904/1‑101 (MQ=255)


                                                                                            cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt         <  1:875652/101‑1 (MQ=255)


                                                                                             gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc        <  2:1739649/101‑1 (MQ=255)


                                                                                               cgcTTTGGCGGTCCACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGc      <  1:1554760/101‑1 (MQ=255)


                                                                                                gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt     >  1:3657427/1‑101 (MQ=255)


                                                                                                 cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGCCCAGCCGGCCTGCTCGTCGCTc    >  1:1832802/1‑101 (MQ=255)


                                                                                                   ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc  >  2:1795288/1‑101 (MQ=255)


                                                                                                   ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc  >  2:2302729/1‑101 (MQ=255)


                                                                                                   ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc  <  2:1457832/101‑1 (MQ=255)


                                                                                                   ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccc  >  2:2384027/1‑101 (MQ=255)


CGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCC  >  NC_014034/1395376‑1395575

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG