Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,562 T→C 25.1% intergenic (+374/‑557) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5620T→C25.1% 180.4 ‑2.1 ‑0.6366intergenic (+374/‑557)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/45):  ref base (108/135):  total (152/214)
Fisher's exact test strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.73e-01

Only 100 of 382 total aligned reads displayed.

AAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395462‑1395660
                                                                                                       |                                                                                                   
   cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCt                                                                                                 <  1:3444660/101‑1 (MQ=255)
   cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCt                                                                                                 >  2:2980976/1‑101 (MQ=255)
    aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCtc                                                                                                <  1:4107146/101‑1 (MQ=255)
      cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCtcgt                                                                                              >  2:336874/1‑101 (MQ=255)
       gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCtcgtc                                                                                             <  2:1739649/101‑1 (MQ=255)
         cgcTTTGGCGGTCCACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGc                                                                                           <  1:1554760/101‑1 (MQ=255)
           cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTc                                                                                         >  1:2256477/1‑101 (MQ=255)
             ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTccc                                                                                       >  2:2302729/1‑101 (MQ=255)
             ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTccc                                                                                       >  2:2384027/1‑101 (MQ=255)
               ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTccccc                                                                                     >  2:4185892/1‑101 (MQ=255)
                 gtgtggataGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCcg                                                                                   <  2:401108/93‑1 (MQ=255)
                  ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCcgc                                                                                  >  1:4887453/1‑101 (MQ=255)
                     cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                                               >  2:2620783/1‑101 (MQ=255)
                       aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                             >  1:351413/1‑101 (MQ=255)
                        cAGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                            <  1:3597768/101‑1 (MQ=255)
                           gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCCCCCCCCCCGGGGGCggg                                                                         >  2:2161716/1‑99 (MQ=255)
                            cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                        >  1:4234010/1‑101 (MQ=255)
                              ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  1:28874/93‑1 (MQ=2)
                              ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:2300646/1‑101 (MQ=255)
                              ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  1:4017875/101‑1 (MQ=255)
                              ggTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  1:1795421/101‑1 (MQ=255)
                               gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1988851/94‑1 (MQ=2)
                               gTAACGTGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1676331/101‑1 (MQ=255)
                               gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  1:2212397/1‑101 (MQ=255)
                               gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:1671381/101‑1 (MQ=255)
                               ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1714703/94‑1 (MQ=2)
                                tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    >  2:2841841/7‑101 (MQ=2)
                                tAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:5693/101‑1 (MQ=255)
                                ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:4752711/95‑1 (MQ=2)
                                ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    >  1:4553690/7‑101 (MQ=2)
                                 aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  1:3977120/1‑101 (MQ=255)
                                 aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATcaa                                                                   >  2:2212785/1‑99 (MQ=255)
                                  gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:3695411/97‑1 (MQ=2)
                                  aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:433048/1‑101 (MQ=255)
                                   cgcgtCGCCCCCCCCGGGGGGGCGTGGATCGAAACACCCCGAACCTGTCG.GCG.GCGAACACCG.ATA.CCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:2752067/98‑1 (MQ=2)
                                    tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                <  1:3168975/99‑1 (MQ=2)
                                    tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                <  1:3772024/99‑1 (MQ=2)
                                    tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                <  2:1546071/99‑1 (MQ=2)
                                    cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                <  2:3173527/99‑1 (MQ=2)
                                     tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                               <  2:1931878/101‑1 (MQ=255)
                                     tgtCGCTCCCCCCGCAGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                               >  1:3362778/1‑101 (MQ=255)
                                     cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                               <  2:3899138/100‑2 (MQ=2)
                                      gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGC.CGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                               <  2:3020265/101‑2 (MQ=2)
                                       tCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.TCCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                             <  2:4508980/101‑4 (MQ=2)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                             <  1:5053765/101‑4 (MQ=2)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                             <  1:4517310/101‑3 (MQ=2)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                           >  1:2141749/1‑96 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCtt                                                          >  1:1927380/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                                          >  1:640531/1‑96 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                          <  2:4982795/101‑7 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                         <  1:2974364/101‑1 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                         >  1:4601225/1‑94 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                         <  1:2438192/101‑8 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                         >  1:1176760/1‑94 (MQ=2)
                                           cccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                         <  1:3062797/100‑8 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                                        <  1:1300738/101‑9 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                        >  2:4398618/1‑94 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                        >  1:879297/1‑93 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                        <  2:4805210/101‑9 (MQ=2)
                                             cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                                       >  1:2126314/1‑92 (MQ=2)
                                             cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                                       >  2:3079507/1‑93 (MQ=0)
                                                  gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                                  <  1:4928471/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                                <  2:202747/101‑1 (MQ=255)
                                                     ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                               <  2:182113/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                             <  2:149210/101‑1 (MQ=255)
                                                        gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                            <  1:980981/101‑1 (MQ=255)
                                                         cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTTTCACttt                                           >  2:4627674/1‑101 (MQ=255)
                                                         cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                           <  1:1018856/101‑1 (MQ=255)
                                                         cGTGGATCAAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                           <  1:1507009/101‑1 (MQ=255)
                                                          gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                          <  2:4233693/101‑1 (MQ=255)
                                                          gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                          >  1:587675/1‑101 (MQ=255)
                                                           tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                         >  2:3419674/1‑101 (MQ=255)
                                                            ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCAGCTTGCGGATTGTCCCTTTcgc                                        >  2:2106032/1‑101 (MQ=255)
                                                              aTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                                      >  1:2587850/1‑101 (MQ=255)
                                                                cGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                                    <  2:2484409/101‑1 (MQ=255)
                                                                cGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                                    >  1:1613284/1‑101 (MQ=255)
                                                                 gAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                                   >  2:2733277/1‑101 (MQ=255)
                                                                      aTCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                              >  1:3862807/1‑101 (MQ=255)
                                                                       tctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                             >  2:1080119/1‑101 (MQ=255)
                                                                        ctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt                            <  2:1301103/101‑1 (MQ=255)
                                                                        ctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt                            >  2:1621199/1‑101 (MQ=255)
                                                                         tcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                           <  1:3305611/101‑1 (MQ=255)
                                                                          cGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                          >  2:444413/1‑101 (MQ=255)
                                                                           ggTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc                         >  1:3925045/1‑101 (MQ=255)
                                                                            gTGCCGTCGCTCG.TCCAGC.CGG.CCT.TCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                        <  2:902090/101‑1 (MQ=255)
                                                                            gTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                        <  2:2653474/101‑1 (MQ=255)
                                                                              gCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                      >  2:3567174/1‑101 (MQ=255)
                                                                              gCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                      >  1:1860764/1‑101 (MQ=255)
                                                                              gCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                      <  1:1533391/101‑1 (MQ=255)
                                                                              gCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                      >  1:734552/1‑101 (MQ=255)
                                                                                 gTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc                   >  2:3762553/1‑101 (MQ=255)
                                                                                  tCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc                  <  2:684100/101‑1 (MQ=255)
                                                                                        g.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg            <  1:4444244/101‑1 (MQ=255)
                                                                                           ccAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc          <  1:4186209/101‑1 (MQ=255)
                                                                                           ccAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc          <  1:1074913/101‑1 (MQ=255)
                                                                                               c.cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg      >  2:3621491/1‑101 (MQ=255)
                                                                                               c.cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg      <  1:4820175/101‑1 (MQ=255)
                                                                                                 cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:3857583/101‑1 (MQ=255)
                                                                                                  gg.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:3246873/101‑1 (MQ=255)
                                                                                                   g.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:4387688/101‑1 (MQ=255)
                                                                                                       |                                                                                                   
AAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395462‑1395660

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG