Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,668 T→C 9.3% intergenic (+480/‑451) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6680T→C 9.3% 45.2 ‑1.7 ‑0.1250intergenic (+480/‑451)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/14):  ref base (125/100):  total (135/115)
Fisher's exact test strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.22e-01

Only 100 of 273 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395568‑1395768
                                                                                                      |                                                                                                      
tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCt                                                                                                        >  1:4747356/1‑101 (MQ=255)
tcgCCCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTCACGTT.TTGGGACG..GAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCtt                                                                                                       <  1:2903929/101‑2 (MQ=2)
 cgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTg                                                                                                       >  1:3815286/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTTACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                      >  1:3144265/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                      >  2:3610793/1‑101 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCa                                                                                                     <  1:2302893/101‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCCAAGCGTCGCTCCCCCCGCGGGGGAGTGGCTGGAAACCTCTGCa                                                                                                     >  2:4118483/1‑101 (MQ=38)
    tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                    >  2:1794229/1‑101 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                <  1:4438630/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                             >  2:1744567/1‑101 (MQ=255)
              ggggCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                          >  2:59174/1‑101 (MQ=255)
               gggCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcgg                                                                                         >  1:5004877/1‑101 (MQ=255)
                 gCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.Gc                                                                                      <  2:222938/101‑1 (MQ=255)
                  cGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCa                                                                                     >  2:299656/1‑101 (MQ=255)
                    tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   >  2:2087524/1‑101 (MQ=255)
                    tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   >  1:1488893/1‑101 (MQ=255)
                    tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   >  1:970804/1‑101 (MQ=255)
                    tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAgg                                                                                   <  2:2370631/101‑2 (MQ=255)
                     ggATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  >  1:216933/1‑101 (MQ=255)
                     ggATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  <  1:1615724/101‑1 (MQ=255)
                     ggATCGAAAACACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  >  2:3704904/1‑101 (MQ=255)
                       aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  1:1499738/101‑1 (MQ=255)
                         cGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.g                                                                             >  2:1916211/1‑101 (MQ=255)
                          gAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            >  2:3124007/1‑101 (MQ=255)
                          gAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            <  1:522476/101‑1 (MQ=255)
                           aaaCCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gcc                                                                           <  2:725296/101‑1 (MQ=255)
                            aaCCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCg                                                                          <  1:2624974/101‑1 (MQ=255)
                             accaccTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGc                                                                         >  1:1953396/1‑101 (MQ=255)
                             accaccTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGc                                                                         >  1:4305370/1‑101 (MQ=255)
                               ccccTTGCGGAT.T.TTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAg                                                                       <  2:1677199/99‑1 (MQ=255)
                                  cTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGCTCGA.GCCGCAGTCg                                                                    >  1:4360436/1‑101 (MQ=255)
                                     gCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                 >  1:2694637/1‑101 (MQ=255)
                                      cGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcc                                                                <  1:2733478/101‑1 (MQ=255)
                                       ggAT.T.GTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTccc                                                               <  1:119712/101‑1 (MQ=255)
                                        gAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                              >  1:2536429/1‑101 (MQ=255)
                                          t.t.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            >  2:4413192/1‑101 (MQ=255)
                                          t.t.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                            >  2:837391/1‑101 (MQ=255)
                                          t.t.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGGTCGA.GCCGCAGTCGCTcccccc                                                            >  2:4830026/1‑101 (MQ=255)
                                            t.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                           <  2:2587669/101‑1 (MQ=255)
                                              gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          >  1:325436/1‑101 (MQ=255)
                                              gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          <  2:4026298/101‑1 (MQ=255)
                                                  cTTTCGCGGGGCGAGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                      <  2:4204907/101‑1 (MQ=255)
                                                  cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                      >  2:4622686/1‑101 (MQ=255)
                                                   tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                     >  1:2062927/1‑101 (MQ=255)
                                                    ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                    >  1:1020281/1‑101 (MQ=255)
                                                      cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                  >  1:4545643/1‑101 (MQ=255)
                                                       tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                  <  1:31130/91‑1 (MQ=2)
                                                        gggggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:1100674/100‑1 (MQ=255)
                                                        cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:4949503/1‑101 (MQ=255)
                                                         ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  1:3762838/101‑1 (MQ=255)
                                                         ggggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  2:4360119/101‑1 (MQ=255)
                                                           ggggAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:837493/98‑1 (MQ=255)
                                                           ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATTTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:3336160/101‑1 (MQ=255)
                                                            gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  1:720017/101‑1 (MQ=255)
                                                            ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:2234570/96‑1 (MQ=2)
                                                             cGAAGCGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTTGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGGTCGa                                           >  1:4260684/1‑101 (MQ=255)
                                                             cGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           <  1:3419938/101‑1 (MQ=255)
                                                              gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  1:2233440/4‑101 (MQ=2)
                                                              gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  1:4118330/4‑101 (MQ=2)
                                                               ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:2016318/99‑1 (MQ=2)
                                                               aaGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:2088558/101‑1 (MQ=255)
                                                                aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:393591/1‑101 (MQ=255)
                                                                aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:3435013/1‑101 (MQ=255)
                                                                aGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        <  1:2762736/101‑1 (MQ=255)
                                                                 gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACt                                       >  2:3517962/1‑101 (MQ=255)
                                                                  ccgcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                      <  2:1389154/98‑1 (MQ=255)
                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                     >  2:2690386/1‑101 (MQ=255)
                                                                    tcccTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                    <  1:3689995/98‑1 (MQ=255)
                                                                     cccTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                   <  2:1125061/99‑1 (MQ=255)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                 <  2:3657155/101‑1 (MQ=255)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                 >  1:4599649/1‑101 (MQ=255)
                                                                        cccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  1:1760071/100‑1 (MQ=255)
                                                                        cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  1:2108345/100‑1 (MQ=255)
                                                                        cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  2:1599850/100‑1 (MQ=255)
                                                                        cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  1:2853699/100‑1 (MQ=255)
                                                                        cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                <  2:216899/100‑1 (MQ=255)
                                                                          cccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                              >  2:1041889/1‑101 (MQ=255)
                                                                          cccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                              <  1:3621762/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  1:3567444/101‑1 (MQ=255)
                                                                               gcgGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         <  2:325383/101‑1 (MQ=255)
                                                                                 gggggGCTGGACCGAAATATCTGCGTGTAGTCGGGGG.GCTGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                       <  2:3835088/101‑1 (MQ=25)
                                                                                  ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                      >  1:170856/1‑101 (MQ=255)
                                                                                    ggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                    >  1:728828/1‑101 (MQ=255)
                                                                                    ggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                    >  2:521171/1‑101 (MQ=255)
                                                                                        tGGCTCGAAACATCTGCTTGTAGGCGGCGG.GGAGTGGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAGCTGGCGCAAACGGa                <  1:334782/101‑1 (MQ=25)
                                                                                        tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  1:1350671/1‑101 (MQ=255)
                                                                                         ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               <  1:4171172/101‑1 (MQ=255)
                                                                                          gATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              <  1:2981204/101‑1 (MQ=255)
                                                                                           aTCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg             <  2:1310250/101‑1 (MQ=255)
                                                                                            tCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt            >  1:2426981/1‑101 (MQ=255)
                                                                                            tCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt            >  1:2593528/1‑101 (MQ=255)
                                                                                             cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:2767760/1‑101 (MQ=255)
                                                                                             cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:4527218/1‑101 (MQ=255)
                                                                                             cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGCATGGATCTGGCGCAAACGGAAAGTc           >  2:291157/1‑101 (MQ=255)
                                                                                              gAAACATCTGCATGTAGTGGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGCCt          >  2:2138198/1‑101 (MQ=255)
                                                                                              gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          >  2:4286436/1‑101 (MQ=255)
                                                                                               aaaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg         <  1:89249/101‑1 (MQ=255)
                                                                                                    tCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc    >  2:2678765/1‑101 (MQ=255)
                                                                                                      tGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc  >  2:2857642/1‑101 (MQ=255)
                                                                                                      tGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc  >  2:2260291/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395568‑1395768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG