Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,744 G→C 10.2% intergenic (+556/‑375) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7440G→C10.2% 64.2 ‑1.4 ‑0.0284intergenic (+556/‑375)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/18):  ref base (128/127):  total (139/145)
Fisher's exact test strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 310 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCG  >  NC_014034/1395644‑1395844
                                                                                                       |                                                                                                         
 gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gc                                                                                                          >  1:1235156/1‑101 (MQ=255)
 gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gc                                                                                                          <  1:3567444/101‑1 (MQ=255)
 gcgGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gc                                                                                                          <  2:325383/101‑1 (MQ=255)
    ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCa                                                                                                       >  2:3915629/1‑101 (MQ=255)
      ggggTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                     <  1:3068081/98‑1 (MQ=255)
      ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                     >  2:521171/1‑101 (MQ=255)
      ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                     >  1:728828/1‑101 (MQ=255)
       gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAAc                                                                                                    <  2:2212242/101‑1 (MQ=255)
          tGGCTCGAAACATCTGCTTGTAGGCGGCGGGGAGTGGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGAGCTG..GCGCAAACGG..a                                                                                               <  1:334782/101‑1 (MQ=25)
          tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..a                                                                                               >  1:4838796/1‑101 (MQ=255)
           ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aa                                                                                              >  2:2285565/1‑101 (MQ=255)
            gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aaa                                                                                             >  1:138248/1‑101 (MQ=255)
            gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aaa                                                                                             >  2:3603901/1‑101 (MQ=255)
               cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTc                                                                                          >  1:516782/1‑101 (MQ=255)
                gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCt                                                                                         >  2:1896613/1‑101 (MQ=255)
                 aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g                                                                                       >  1:2734176/1‑101 (MQ=255)
                 aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g                                                                                       <  1:220889/101‑1 (MQ=255)
                   aCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                    >  1:1928921/1‑101 (MQ=255)
                      tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGCCT.g.ccgtc                                                                                 >  2:3679848/1‑101 (MQ=255)
                       cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                >  1:3700067/1‑101 (MQ=255)
                            ggTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AACGTCT.G.TCGTCGCT.ccc                                                                          <  1:4413511/100‑1 (MQ=255)
                             gTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.cccc                                                                         <  1:1161287/101‑1 (MQ=255)
                             gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.cccc                                                                         <  2:4599327/101‑1 (MQ=255)
                                gTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCcg                                                                      <  1:3210529/101‑1 (MQ=255)
                                   ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCgg                                                                   >  2:2121547/1‑101 (MQ=255)
                                      gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCggggg                                                                >  1:4037085/1‑101 (MQ=255)
                                      gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCggggg                                                                >  1:4889940/1‑101 (MQ=255)
                                      gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCggggg                                                                >  1:3318497/1‑101 (MQ=255)
                                       ggCGGTCCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGc                                                               <  1:3888888/101‑1 (MQ=255)
                                        gCAGTGCGAGCCGCAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCCGCGGGGGc                                                               <  2:2617497/101‑1 (MQ=38)
                                          aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTg                                                            <  1:323472/101‑1 (MQ=255)
                                          aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCN.CCCCCCGCGGGGGCGTg                                                            >  2:1196052/1‑101 (MQ=255)
                                             tCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGAt                                                         >  1:2637204/1‑101 (MQ=255)
                                              cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                        <  2:721739/101‑1 (MQ=255)
                                                 gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                        >  2:1027640/5‑101 (MQ=2)
                                                 gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.GGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                        >  2:506593/5‑101 (MQ=2)
                                                 gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:4364384/101‑1 (MQ=255)
                                                   ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTACGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGCGGATCGa                                                      >  1:4263048/3‑101 (MQ=0)
                                                   ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGa                                                      <  1:2681320/99‑1 (MQ=2)
                                                    tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:3778276/2‑101 (MQ=2)
                                                    gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcc                                                  >  2:4111666/1‑101 (MQ=255)
                                                    ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                    <  2:4923984/98‑1 (MQ=2)
                                                     tggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:1387470/99‑1 (MQ=2)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:4756481/101‑1 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAca                                                  >  1:1683291/1‑100 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgc                                               >  2:4007226/1‑101 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgc                                               >  1:1314846/1‑101 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATGGATCTG..GCGTAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgc                                               <  1:2830236/101‑1 (MQ=255)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcac                                                 <  1:2535894/101‑3 (MQ=2)
                                                        tCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                              <  1:4286753/101‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGTGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtat                                               <  2:4539446/101‑3 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacc                                                <  1:1849072/101‑4 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtat                                               <  2:4289950/101‑3 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtat                                               <  1:4949381/101‑3 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                             >  1:452418/1‑101 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGGCT.G.TCCTCGCT.CCCCCCGCGGGGGCGGGGCTCGAAAGCTgtgg                                             >  2:4184789/1‑101 (MQ=25)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcaccc                                               >  2:4984721/1‑97 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                              >  2:3416623/1‑98 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                              <  1:1057341/101‑4 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                              <  1:155601/101‑4 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatg                                              <  1:3994964/101‑4 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                            <  1:618059/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccg                                              <  2:4893434/101‑6 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccg                                              >  2:2897307/1‑96 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatga                                             <  1:4987852/101‑5 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcgtatgag                                            <  1:2066794/101‑8 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                            <  1:3037247/101‑6 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                            >  2:3106645/1‑96 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                            >  1:2439499/1‑96 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                          >  2:1599849/1‑100 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                            <  2:4338879/101‑8 (MQ=0)
                                                             ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                            >  1:2159312/1‑94 (MQ=0)
                                                             ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                         >  1:84409/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                         <  2:1530746/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                        <  2:3727125/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAgatc                                    >  2:5017295/1‑98 (MQ=255)
                                                                     gggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                                 >  1:2319675/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                                >  2:1964466/1‑101 (MQ=255)
                                                                       gggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAgg                               >  2:2848348/1‑100 (MQ=255)
                                                                        ggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                              <  2:3359435/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGCGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                              >  1:3621578/1‑101 (MQ=255)
                                                                         gCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGa                             <  2:2694452/101‑1 (MQ=255)
                                                                          cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                            <  1:2384191/101‑1 (MQ=255)
                                                                           gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                           >  2:2752649/1‑101 (MQ=255)
                                                                             ggATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                         >  1:4601131/1‑101 (MQ=255)
                                                                              gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGACAGAc                        <  2:4273559/101‑1 (MQ=255)
                                                                                tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                      <  1:4627997/101‑1 (MQ=255)
                                                                                  gAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                    >  2:2298444/1‑101 (MQ=255)
                                                                                   aaaCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                   >  2:1840929/1‑101 (MQ=255)
                                                                                     aCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGCGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                 >  1:2971400/1‑101 (MQ=255)
                                                                                           tATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc            >  1:3443522/1‑101 (MQ=255)
                                                                                            aTGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg           <  2:2016822/101‑1 (MQ=255)
                                                                                              ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGTCGTCGCt         >  2:4501529/1‑101 (MQ=255)
                                                                                              ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt         >  1:3807805/1‑101 (MQ=255)
                                                                                                aTCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc       >  2:2225371/1‑101 (MQ=255)
                                                                                                   tG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccc    <  2:3862515/101‑1 (MQ=255)
                                                                                                    g..gCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc   >  2:4673375/1‑101 (MQ=255)
                                                                                                    g..gCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc   <  1:929284/101‑1 (MQ=255)
                                                                                                    g..gCGCAAACGG..AAAGTCT.G.TCGTCGCC.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc   <  1:2115748/101‑1 (MQ=255)
                                                                                                       gcgcAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccccc  >  2:352936/1‑100 (MQ=255)
                                                                                                       |                                                                                                         
CGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCG  >  NC_014034/1395644‑1395844

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG