BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,755	A→C	10.3%	intergenic (+567/‑364)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,755	0	A→C	10.3%	67.8 ‑1.1 ‑0.0	287	intergenic (+567/‑364)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (12/18): ref base (129/128): total (141/146)
Fisher's exact test strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.17e-01

Only 100 of 310 total aligned reads displayed.


GGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395655‑1395855


 ggtCGAAGCATCTGGTGGTGGGCGGCGGGCGGTTGGAGCCGGAGTGGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aaa                                                                                                       <  1:3611064/99‑1 (MQ=21)


 gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aaa                                                                                                       >  1:138248/1‑101 (MQ=255)


 gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aaa                                                                                                       >  2:3665253/1‑101 (MQ=255)


  aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAg                                                                                                      <  2:3590980/101‑1 (MQ=255)


   tCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGt                                                                                                     >  1:2593528/1‑101 (MQ=255)


   tCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGt                                                                                                     >  1:2426981/1‑101 (MQ=255)


    cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTc                                                                                                    >  2:2767760/1‑101 (MQ=255)


    cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTc                                                                                                    <  2:2886316/101‑1 (MQ=255)


     gAAACATCTGCATGTAGTGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGCCt                                                                                                   >  2:2138198/1‑101 (MQ=255)


       aaCATCTGCATGTGGTGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.t                                                                                               <  1:2106180/101‑1 (MQ=255)


         cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcg                                                                                             <  1:4909450/101‑1 (MQ=255)


         cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcg                                                                                             >  1:2817565/1‑101 (MQ=255)


         cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcg                                                                                             >  2:4155464/1‑101 (MQ=255)


             tGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGc                                                                                         >  2:2260291/1‑101 (MQ=255)


                 ggTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AACGTCT.G.TCGTCGCTccc                                                                                     <  1:4413511/100‑1 (MQ=255)


                  gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                                    >  1:3087414/1‑101 (MQ=255)


                    ggTCGGCGGGCAGTCCGAGCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                                  <  2:516703/100‑1 (MQ=255)


                     gTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcg                                                                                 <  1:3210529/101‑1 (MQ=255)


                      tgggggggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                                                <  2:4502216/96‑1 (MQ=255)


                       cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgcg                                                                               >  2:5037830/1‑101 (MQ=255)


                           gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                           >  1:4037085/1‑101 (MQ=255)


                               aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                       >  2:3345035/1‑101 (MQ=255)


                               aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCNCCCCCCGCGGGGGCGTg                                                                       >  2:1196052/1‑101 (MQ=255)


                                gTTGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  1:3915921/101‑1 (MQ=255)


                                gTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  2:3049997/1‑101 (MQ=255)


                                  tcaagattcAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                                       <  2:3251915/93‑1 (MQ=2)


                                  tCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  2:4982523/1‑101 (MQ=255)


                                  ccGAGCCGCCGTCGCCCCCCCCGCGGGGGCGTTGATCGAAACT.GGTATGGATCT.G..GCCCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:2138346/100‑1 (MQ=38)


                                   cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:721739/101‑1 (MQ=255)


                                    gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:98905/93‑1 (MQ=0)


                                    aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:2524565/95‑1 (MQ=2)


                                    aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:3662728/95‑1 (MQ=2)


                                      gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:851139/95‑1 (MQ=0)


                                       ccGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  1:4527538/101‑1 (MQ=255)


                                       attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG..GCGATCTGGGT.AAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:2002408/4‑101 (MQ=2)


                                       attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:60664/98‑1 (MQ=2)


                                        ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTACGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGCGGATCGa                                                                 >  1:4263048/3‑101 (MQ=0)


                                        ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:2681320/99‑1 (MQ=2)


                                        ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  2:4752545/99‑1 (MQ=2)


                                         tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                                                >  2:2672616/2‑99 (MQ=2)


                                          tggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  2:1387470/99‑1 (MQ=2)


                                          cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATcagat                                                               >  2:4611981/1‑97 (MQ=2)


                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                             >  2:2289756/2‑101 (MQ=2)


                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                             >  1:130646/2‑101 (MQ=2)


                                           aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                           >  1:2347934/1‑101 (MQ=255)


                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                          <  2:3744672/101‑1 (MQ=255)


                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                          >  2:4007226/1‑101 (MQ=255)


                                            gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGAca                                                             <  1:1896147/101‑2 (MQ=2)


                                             tCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                                         <  1:4286753/101‑1 (MQ=255)


                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                          <  2:4289950/101‑3 (MQ=2)


                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                                        >  2:4903140/1‑101 (MQ=255)


                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                         <  2:5007659/101‑4 (MQ=2)


                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                         >  1:3715007/1‑98 (MQ=2)


                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                         >  1:4871950/1‑98 (MQ=2)


                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                                       >  1:3488700/1‑101 (MQ=255)


                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                                       <  1:1744697/101‑1 (MQ=255)


                                                cTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcg                                                      >  1:2267527/1‑101 (MQ=255)


                                                 tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                       <  1:3037247/101‑6 (MQ=2)


                                                 tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                                     >  2:1599849/1‑100 (MQ=255)


                                                 cccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                        <  2:2086059/100‑7 (MQ=0)


                                                 cccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                        <  2:3894399/100‑7 (MQ=0)


                                                  gatctcgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                    <  1:5017625/96‑1 (MQ=255)


                                                  ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                       <  2:4338879/101‑8 (MQ=0)


                                                  ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                    <  2:1530746/101‑1 (MQ=255)


                                                  ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                    <  2:4576296/101‑1 (MQ=255)


                                                  ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCTGCGGCGTg                                                    >  1:2194961/1‑101 (MQ=255)


                                                    ccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAg                                                  <  1:3810210/101‑1 (MQ=255)


                                                      ccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGtt                                                <  2:3792730/101‑1 (MQ=255)


                                                       cgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                                               <  1:3435277/101‑1 (MQ=255)


                                                           ggggCGTGGATCGAAACT.GGTACGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                                           >  2:2219794/1‑101 (MQ=255)


                                                               cGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                       <  2:2696841/101‑1 (MQ=255)


                                                                gTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                      >  2:1974341/1‑101 (MQ=255)


                                                                 tGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                                     <  1:274270/101‑1 (MQ=255)


                                                                 tGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                                     >  1:728176/1‑101 (MQ=255)


                                                                  ggATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                                    >  1:4601131/1‑101 (MQ=255)


                                                                   gATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                                   <  2:4838469/101‑1 (MQ=255)


                                                                   gATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGACAGAc                                   <  2:4273559/101‑1 (MQ=255)


                                                                     tCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                                 <  1:4627997/101‑1 (MQ=255)


                                                                       gAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                               >  1:3327564/1‑101 (MQ=255)


                                                                        aaaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCgg                              >  1:1093676/1‑100 (MQ=255)


                                                                        aaaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                              >  2:1840929/1‑101 (MQ=255)


                                                                          aCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                            >  1:2971400/1‑101 (MQ=255)


                                                                               gTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTTGACGt                        <  2:1928784/101‑1 (MQ=255)


                                                                                  tGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGc                     >  2:4436599/1‑101 (MQ=255)


                                                                                   ggATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTCATAGACGCCGTGGACGTCGCt                    >  2:3399631/1‑101 (MQ=255)


                                                                                          g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc              >  1:4010479/1‑101 (MQ=255)


                                                                                          g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc              <  1:4949833/101‑1 (MQ=255)


                                                                                             gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccccc             >  2:352936/1‑100 (MQ=255)


                                                                                             gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcg             <  2:369860/101‑1 (MQ=255)


                                                                                                cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgg          >  1:1500376/1‑101 (MQ=255)


                                                                                                 aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggg         >  1:267426/1‑101 (MQ=255)


                                                                                                   aCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggggg       >  2:4205555/1‑101 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggggcc      >  2:2355307/1‑99 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc      >  1:4783394/1‑101 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc      >  1:1614343/1‑101 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc      >  1:1230956/1‑101 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc      <  1:1008604/101‑1 (MQ=255)


                                                                                                    cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCCCGGGGGc      >  1:907393/1‑101 (MQ=255)


                                                                                                         aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTg   >  2:4363281/1‑101 (MQ=255)


                                                                                                          aaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTgg  >  1:3237075/1‑101 (MQ=255)


GGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395655‑1395855

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG