BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,758	T→C	18.7%	intergenic (+570/‑361)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,758	0	T→C	18.7%	116.0 ‑0.9 ‑0.9	288	intergenic (+570/‑361)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (20/26): ref base (109/104): total (140/148)
Fisher's exact test strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.15e-01

Only 100 of 301 total aligned reads displayed.


TCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395658‑1395858


 cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTc                                                                                                        >  2:4527218/1‑101 (MQ=255)


 cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTc                                                                                                        <  2:2886316/101‑1 (MQ=255)


 cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTc                                                                                                        >  2:2767760/1‑101 (MQ=255)


   aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.g                                                                                                     >  1:3585406/1‑101 (MQ=255)


   aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.g                                                                                                     <  1:89249/101‑1 (MQ=255)


       ctCTGCATGTGGCGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.g..tcgt                                                                                               <  2:1548907/100‑1 (MQ=255)


        tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGCCT.g..ccgtc                                                                                              >  2:3679848/1‑101 (MQ=255)


         cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..tcgtcg                                                                                             >  1:3700067/1‑101 (MQ=255)


         cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..tcgtcg                                                                                             >  1:2398007/1‑101 (MQ=255)


             aTGTAGTCGGCGGGAAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTcc                                                                                         <  1:3124245/101‑1 (MQ=255)


               gTAGTCGGCGGGAAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTcccc                                                                                       <  2:4215985/101‑1 (MQ=255)


                 ggTCGGCGGGCAGTCCGAGCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTcccccc                                                                                     <  2:516703/100‑1 (MQ=255)


                 cggcggcggGCAGTTCGACCCGCTGTCGCTGCCCCCGCGGGGGCGTGGATCTAAACT.GGTTTG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTcccccc                                                                                     <  1:798152/98‑1 (MQ=38)


                    cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCcgcg                                                                                  >  2:5037830/1‑101 (MQ=255)


                    cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCcgcg                                                                                  >  1:2091024/1‑101 (MQ=255)


                     ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCgg                                                                                 >  2:2121547/1‑101 (MQ=255)


                     ggcggGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCgg                                                                                 <  2:3864247/101‑1 (MQ=255)


                        gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCggggg                                                                              >  1:4889940/1‑101 (MQ=255)


                        gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCggggg                                                                              >  1:3318497/1‑101 (MQ=255)


                         ggCGGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGc                                                                             <  2:3378504/101‑1 (MQ=255)


                         ggCGGTCCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGc                                                                             <  1:3888888/101‑1 (MQ=255)


                           cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGt                                                                           <  1:2690571/101‑1 (MQ=255)


                            aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTg                                                                          <  1:323472/101‑1 (MQ=255)


                             gtgcGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:2186852/98‑1 (MQ=255)


                               tCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:4982523/1‑101 (MQ=255)


                                cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:721739/101‑1 (MQ=255)


                                 gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:2328698/101‑1 (MQ=255)


                                   gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  2:1027640/5‑101 (MQ=2)


                                   gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  2:4364384/101‑1 (MQ=255)


                                    ccGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:4527538/101‑1 (MQ=255)


                                    attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATCG..GCGATCTGGGT.AAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:2002408/4‑101 (MQ=2)


                                    attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:4809219/98‑1 (MQ=2)


                                    attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:212518/4‑101 (MQ=2)


                                     ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:4752545/99‑1 (MQ=2)


                                     cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  1:69988/101‑1 (MQ=255)


                                      tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                                                   >  2:2672616/2‑99 (MQ=2)


                                      tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  2:3778276/2‑101 (MQ=2)


                                      gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                >  2:4111666/1‑101 (MQ=255)


                                       cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATcagat                                                                  >  2:4611981/1‑97 (MQ=2)


                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                >  1:130646/2‑101 (MQ=2)


                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                >  2:2289756/2‑101 (MQ=2)


                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                              <  2:1488763/101‑1 (MQ=255)


                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                               <  2:4361923/101‑1 (MQ=2)


                                         gTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATG.GATCT.G..GCGTAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                             <  1:2830236/101‑1 (MQ=255)


                                         gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGAca                                                                <  1:1896147/101‑2 (MQ=2)


                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                                            >  2:123139/1‑101 (MQ=255)


                                          gcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAAA.T..CCGCAATCGG..CCGGGCT.GGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                              <  1:4264405/100‑2 (MQ=2)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                             <  1:4949381/101‑3 (MQ=2)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                                           <  1:4564855/101‑1 (MQ=255)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                                           >  2:4903140/1‑101 (MQ=255)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgcgg                                                           >  1:2222896/1‑101 (MQ=255)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGGCT.G..TCCTCGCTCCCCCCGCGGGGGCGGGGCTCGAAAGCTgtgg                                                           >  2:4184789/1‑101 (MQ=25)


                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                             <  1:3638760/101‑5 (MQ=2)


                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                            <  2:5007659/101‑4 (MQ=2)


                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                            <  1:155601/101‑4 (MQ=2)


                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                            <  1:3994964/101‑4 (MQ=2)


                                             ccccccccGGGGGGGCGTGGATCGAAACTCGGCAAG.GACAT.C..GCCGATCTGGGTAAGGCCG.G..TGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                            <  1:2651617/99‑6 (MQ=2)


                                             cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCA.GATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                           <  1:4987852/101‑5 (MQ=2)


                                               ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                       <  2:4576296/101‑1 (MQ=255)


                                                cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                                      <  2:3727125/101‑1 (MQ=255)


                                                cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCCGCGGCGGGAg                                                     >  2:3491625/1‑101 (MQ=255)


                                                  cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTTAGt                                                    <  2:4274163/101‑1 (MQ=255)


                                                  cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGt                                                    <  1:4623008/101‑1 (MQ=255)


                                                     gcgGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGc                                                 <  2:3127920/101‑1 (MQ=255)


                                                      cgGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                                <  1:2679592/101‑1 (MQ=255)


                                                            cGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                          >  2:2051248/1‑101 (MQ=255)


                                                            cGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                          <  2:2696841/101‑1 (MQ=255)


                                                            cGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                                          <  1:4326667/101‑1 (MQ=255)


                                                             gTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                         >  2:1974341/1‑101 (MQ=255)


                                                              tGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTTCGGCGTGAGTTGCCAGTGATAg                                        <  2:3948830/101‑1 (MQ=255)


                                                                gATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                                      <  2:4838469/101‑1 (MQ=255)


                                                                 aTCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                                     <  1:444478/101‑1 (MQ=255)


                                                                 aTCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                                     >  1:2985520/1‑101 (MQ=255)


                                                                     aaaCT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCgg                                 >  1:1093676/1‑100 (MQ=255)


                                                                     aaaCT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                                 >  2:1840929/1‑101 (MQ=255)


                                                                       aCT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                               <  2:2958186/101‑1 (MQ=255)


                                                                       aCT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                               >  1:1677645/1‑101 (MQ=255)


                                                                        cT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                              >  2:2219080/1‑101 (MQ=255)


                                                                        cT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                              >  1:4036796/1‑101 (MQ=255)


                                                                            gTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTTGACGt                           <  2:1928784/101‑1 (MQ=255)


                                                                             tATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                          >  1:2558902/1‑101 (MQ=255)


                                                                             tATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                          <  2:4305053/101‑1 (MQ=255)


                                                                              aTG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACgctg                         >  1:2485892/1‑98 (MQ=255)


                                                                                g.gATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt                       >  1:3807805/1‑101 (MQ=255)


                                                                                   aTCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc                     <  1:3425773/101‑1 (MQ=255)


                                                                                      t.G..GCGCAAACGG..AAAGTCT.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccc                  <  1:521250/101‑1 (MQ=255)


                                                                                        g..gCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc                 <  2:2233526/101‑1 (MQ=255)


                                                                                        g..gCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc                 <  1:929284/101‑1 (MQ=255)


                                                                                        g..gCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc                 <  1:3064132/101‑1 (MQ=255)


                                                                                              cAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgg             >  1:4738905/1‑101 (MQ=255)


                                                                                                aaCGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgggg           <  2:3585135/101‑1 (MQ=255)


                                                                                                  cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggggcc         >  2:2355307/1‑99 (MQ=255)


                                                                                                  cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc         <  1:3482243/101‑1 (MQ=255)


                                                                                                  cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc         >  1:1230956/1‑101 (MQ=255)


                                                                                                  cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc         <  1:1008604/101‑1 (MQ=255)


                                                                                                    g..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCgg       >  2:4495520/1‑100 (MQ=255)


                                                                                                    g..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCgg       >  1:1446326/1‑100 (MQ=255)


                                                                                                       aaaGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTg      <  1:2752851/101‑1 (MQ=255)


                                                                                                        aaGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTgg     <  2:3329786/101‑1 (MQ=255)


                                                                                                           tCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGGGATAGACGCCGTGGACGTCGCCCCCCCCCCGGGGGCGTGGATc  >  1:1781594/1‑101 (MQ=255)


TCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCT.G..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395658‑1395858

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG