BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,938	T→G	2.5%	intergenic (+750/‑181)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,938	0	T→G	2.5%	7.3 ‑0.4 ‑0.2	238	intergenic (+750/‑181)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/4): ref base (115/115): total (118/120)
Fisher's exact test strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.75e-01

Only 100 of 256 total aligned reads displayed.


CCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTC  >  NC_014034/1395838‑1396038


 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTc                                                                                                     <  2:2267372/101‑1 (MQ=255)


  ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCa                                                                                                    >  2:1013804/1‑101 (MQ=255)


   cccGCGGGGGCTTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGATGC..GTTCAt                                                                                                   <  2:4908757/101‑1 (MQ=255)


   cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCAt                                                                                                   <  1:1964610/101‑1 (MQ=255)


     cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATgg                                                                                                 <  1:2225526/101‑1 (MQ=255)


     cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATgg                                                                                                 >  1:4604887/1‑101 (MQ=255)


      gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGGGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGc                                                                                                <  1:1896750/101‑1 (MQ=255)


       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GGTCATGGcc                                                                                               >  2:1765992/1‑101 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGCGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGACGC..GATCATGGCCt                                                                                              >  2:4275150/1‑101 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCt                                                                                              <  1:4111983/101‑1 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCt                                                                                              <  2:4738582/101‑1 (MQ=255)


        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCt                                                                                              >  1:4295333/1‑101 (MQ=255)


         gtggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTa                                                                                             <  1:1974485/99‑1 (MQ=255)


         ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTa                                                                                             <  1:2837069/101‑1 (MQ=255)


         ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTa                                                                                             <  1:1841062/101‑1 (MQ=255)


         ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTa                                                                                             <  1:2291560/101‑1 (MQ=255)


          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTAc                                                                                            <  2:1829275/101‑1 (MQ=255)


          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTAc                                                                                            <  2:3862789/101‑1 (MQ=255)


           ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACg                                                                                           <  1:352989/101‑1 (MQ=255)


           ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCCACg                                                                                           >  2:888358/1‑101 (MQ=255)


             cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTc                                                                                         >  2:113405/1‑101 (MQ=255)


             cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCACGGCCGACggg                                                                                         >  2:4589108/1‑99 (MQ=255)


               tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCgg                                                                                       >  1:5018700/1‑101 (MQ=255)


                 gATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGc                                                                                     <  2:4006879/101‑1 (MQ=255)


                   tCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAg                                                                                   <  2:4391529/101‑1 (MQ=255)


                     gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                 <  1:2051392/101‑1 (MQ=255)


                     gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                 >  1:4445293/1‑101 (MQ=255)


                      aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                >  1:3130263/1‑101 (MQ=255)


                      aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                <  1:123155/101‑1 (MQ=255)


                      aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGGgg                                                                                >  2:4938969/1‑101 (MQ=255)


                      aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCGCCGCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                >  1:793342/1‑101 (MQ=255)


                       aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                               <  2:3327301/101‑1 (MQ=255)


                       aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                               >  1:1153853/1‑101 (MQ=255)


                       aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTAGa                                                                               <  1:2884298/101‑1 (MQ=255)


                          tGCGCCGCCTAACGCATCCGGTCGAGCGCCGCGCAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTg                                                                            <  2:793240/101‑1 (MQ=255)


                           gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                           <  2:2585300/101‑1 (MQ=255)


                             gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                         <  2:3685649/101‑1 (MQ=255)


                                gccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                      <  1:1627962/101‑1 (MQ=255)


                                gccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                      >  2:3103112/1‑101 (MQ=255)


                                    aTCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                                  >  1:2425144/1‑101 (MQ=255)


                                     tCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                                                                 <  1:3218710/101‑1 (MQ=255)


                                     tCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                                                                 <  2:2637019/101‑1 (MQ=255)


                                     tCGCATCCGGTCGAGCGCCGCGCAGTCGCTACCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTccccc                                                                 >  1:4492324/1‑101 (MQ=255)


                                       gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGGGGACTGGGCGCCccccccc                                                               >  1:3460235/1‑100 (MQ=38)


                                        cATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc                                                              <  1:976344/101‑1 (MQ=255)


                                           ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                           >  2:719639/1‑101 (MQ=255)


                                           ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                           >  1:3830483/1‑101 (MQ=255)


                                           ccGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                           >  2:1565971/1‑101 (MQ=255)


                                              gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                        >  1:1290091/1‑101 (MQ=255)


                                               tCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg                                                       <  1:3987524/101‑1 (MQ=255)


                                                cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                      <  2:2985292/101‑1 (MQ=255)


                                                cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                      >  2:2756360/1‑101 (MQ=255)


                                                 gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                                     >  1:1687840/1‑101 (MQ=255)


                                                   gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                   <  1:3678662/101‑1 (MQ=255)


                                                   gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                   <  1:4903467/101‑1 (MQ=255)


                                                     gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  2:1840048/101‑1 (MQ=255)


                                                     gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GACCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 >  2:4366721/1‑101 (MQ=255)


                                                      acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  1:2597771/94‑1 (MQ=2)


                                                        catccatCGCCCCGCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              >  2:3500882/7‑101 (MQ=37)


                                                         cgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             <  1:1599981/101‑1 (MQ=255)


                                                          gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  1:4398402/101‑1 (MQ=255)


                                                          gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            >  1:4806706/1‑101 (MQ=255)


                                                           cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                           >  2:2065611/1‑101 (MQ=255)


                                                           cAGTCGCTCCCACCGCGGGGGCGTGGATCGAAGCGCGGCAAGCACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCTTCGATCGaaa                                             <  2:308898/101‑1 (MQ=2)


                                                           atgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAAcg                                           >  1:2967903/3‑100 (MQ=2)


                                                            tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgg                                          <  1:631125/100‑3 (MQ=2)


                                                            aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                          <  2:3195784/101‑1 (MQ=255)


                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                         >  1:712807/1‑101 (MQ=255)


                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                         >  2:2239026/1‑101 (MQ=255)


                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                     >  2:369610/1‑101 (MQ=255)


                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTGGGGCAGCGGACGGGTCGCCACCCCCGCGGGGGCGTCGATCGAAAGCCGcccac                                    >  2:5027274/1‑99 (MQ=25)


                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                    <  1:2854932/101‑1 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTCGCCCTCg                                   >  1:837314/1‑101 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                   >  1:4857594/1‑101 (MQ=255)


                                                                   ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                   >  2:1998617/1‑101 (MQ=255)


                                                                        cgcgGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcg                              <  1:2183926/101‑1 (MQ=255)


                                                                         gcgGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                             >  1:2095565/1‑101 (MQ=255)


                                                                          cgGGGGAGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                            >  2:2904946/1‑101 (MQ=255)


                                                                             gggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                         >  2:3262068/1‑101 (MQ=255)


                                                                               gCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                       <  1:1013914/101‑1 (MQ=255)


                                                                                 gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                     >  1:732087/1‑101 (MQ=255)


                                                                                 gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                     >  2:121496/1‑101 (MQ=255)


                                                                                  tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                    >  1:3727840/1‑101 (MQ=255)


                                                                                  tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                    <  2:2869657/101‑1 (MQ=255)


                                                                                  tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                    >  1:1180577/1‑101 (MQ=255)


                                                                                   ggATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                   >  1:150255/1‑101 (MQ=255)


                                                                                     aTCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                 <  2:4126614/101‑1 (MQ=255)


                                                                                     aTCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                 >  2:4415350/1‑101 (MQ=255)


                                                                                      tCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                <  1:1041999/101‑1 (MQ=255)


                                                                                        gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt              >  2:3455376/1‑101 (MQ=255)


                                                                                         aaaGGATGC..GTTCATGGCCTACGGCGGGCAGTGGACTGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg             <  1:4275467/101‑1 (MQ=255)


                                                                                         aaaCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg             >  2:4522897/1‑101 (MQ=255)


                                                                                           aCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc           <  2:2518832/101‑1 (MQ=255)


                                                                                                gC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTc      <  2:1446199/101‑1 (MQ=255)


                                                                                                gC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTc      <  1:4367512/101‑1 (MQ=255)


                                                                                                 c..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg     >  1:3027118/1‑101 (MQ=255)


                                                                                                 c..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg     >  2:544082/1‑101 (MQ=255)


                                                                                                    gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTTCCGCGTCGc    >  2:2833145/1‑101 (MQ=255)


                                                                                                      tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  1:1413128/1‑101 (MQ=255)


                                                                                                      tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc  >  2:3013636/1‑101 (MQ=255)


CCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTC  >  NC_014034/1395838‑1396038

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG