BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,949	G→T	2.5%	intergenic (+761/‑170)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,949	0	G→T	2.5%	7.1 ‑0.9 ‑0.1	236	intergenic (+761/‑170)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/4): ref base (124/104): total (126/110)
Fisher's exact test strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.59e-01

Only 100 of 252 total aligned reads displayed.


GGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395849‑1396049


ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCCACg                                                                                                      >  2:888358/1‑101 (MQ=255)


 gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGt                                                                                                     <  1:3779556/101‑1 (MQ=255)


  cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTc                                                                                                    >  2:113405/1‑101 (MQ=255)


  cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCACGGCCGACggg                                                                                                    >  2:4589108/1‑99 (MQ=255)


    tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCgg                                                                                                  >  1:5018700/1‑101 (MQ=255)


     ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCggg                                                                                                 >  1:396042/1‑101 (MQ=255)


      gATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GCTCATGGCCTACGTCGTGc                                                                                                >  1:1841863/1‑101 (MQ=255)


       aTCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGcc                                                                                               >  2:1793991/1‑100 (MQ=255)


          gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                            <  1:2331528/101‑1 (MQ=255)


          gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTg                                                                                            <  1:2051392/101‑1 (MQ=255)


           aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                           <  1:4048777/101‑1 (MQ=255)


           aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCGCCGCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTgg                                                                                           >  1:793342/1‑101 (MQ=255)


            aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                          >  1:1678944/1‑101 (MQ=255)


            aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                          >  1:1153853/1‑101 (MQ=255)


            aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGa                                                                                          <  2:3327301/101‑1 (MQ=255)


            aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTAGa                                                                                          <  1:2884298/101‑1 (MQ=255)


              cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACt                                                                                        <  2:2652816/101‑1 (MQ=255)


               tGCGCCGCCTAACGCATCCGGTCGAGCGCCGCGCAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTg                                                                                       <  2:793240/101‑1 (MQ=255)


                  gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                                    <  2:3685649/101‑1 (MQ=255)


                  gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                                    <  2:4388267/101‑1 (MQ=255)


                    cgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc                                                                                  <  1:21109/101‑1 (MQ=255)


                        tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                              >  2:2679476/1‑101 (MQ=255)


                         aTCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                                             >  2:31217/1‑101 (MQ=255)


                          tCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                                                                            <  1:3218710/101‑1 (MQ=255)


                           cGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                                           <  1:2775648/101‑1 (MQ=255)


                                   gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGGc                                                                   >  1:4415115/1‑101 (MQ=255)


                                     cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                                 >  2:2756360/1‑101 (MQ=255)


                                     cGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                                 <  2:2985292/101‑1 (MQ=255)


                                      gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCCGTGGCCTGGTCGCCCCCCCCGCGGGGGCggg                                                                >  1:1194560/1‑99 (MQ=38)


                                       aGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                                               >  1:1467214/1‑101 (MQ=255)


                                       aGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGgg                                                               >  1:1607112/1‑101 (MQ=255)


                                        gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:3678662/101‑1 (MQ=255)


                                        gcgcCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:4903467/101‑1 (MQ=255)


                                         gaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:4626638/92‑1 (MQ=2)


                                          gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:1840048/101‑1 (MQ=255)


                                          gccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GACCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            >  2:4366721/1‑101 (MQ=255)


                                          gccgcGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:4004429/101‑1 (MQ=255)


                                             gcgcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:728739/101‑1 (MQ=255)


                                             catccatCGCCCCGCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  2:3500882/7‑101 (MQ=37)


                                              ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGGTCGaaa                                                        >  2:328716/5‑101 (MQ=2)


                                               gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:2553952/101‑1 (MQ=255)


                                               gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:4398402/101‑1 (MQ=255)


                                               gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  1:4806706/1‑101 (MQ=255)


                                                cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                      <  2:168745/101‑1 (MQ=255)


                                                cAGTCGCTCCCACCGCGGGGGCGTGGATCGAAGCGCGGCAAGCACATCGCCGATCTGGGTAAGGCCGGTGGTCGCTCCCCCCGCGGGGGCTTCGATCGaaa                                                        <  2:308898/101‑1 (MQ=2)


                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGC..TGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgg                                                     <  1:631125/100‑3 (MQ=2)


                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                                    >  2:2239026/1‑101 (MQ=255)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                                  >  1:1997426/1‑101 (MQ=255)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                                >  2:369610/1‑101 (MQ=255)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGCCG.GGGTTGGAACCCGCCCTc                                               >  2:1350029/1‑101 (MQ=37)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                               <  1:2854932/101‑1 (MQ=255)


                                                        ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTCGCCCTCg                                              >  1:837314/1‑101 (MQ=255)


                                                        ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                              <  1:726125/101‑1 (MQ=255)


                                                        ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                              >  1:4857594/1‑101 (MQ=255)


                                                        ccccccGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                              >  1:4211571/1‑101 (MQ=255)


                                                             cgcgGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcg                                         <  1:2183926/101‑1 (MQ=255)


                                                               cgGGGGAGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                                       >  2:2904946/1‑101 (MQ=255)


                                                                 ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                     >  1:3607684/1‑101 (MQ=255)


                                                                 ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                     >  2:4420384/1‑101 (MQ=255)


                                                                 ggggCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGAAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                     >  2:4063597/1‑101 (MQ=255)


                                                                    gCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                                  <  1:1013914/101‑1 (MQ=255)


                                                                      gTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGCGCTTGccc                                >  2:4924543/1‑100 (MQ=255)


                                                                       tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                               <  2:2869657/101‑1 (MQ=255)


                                                                       tGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCCCGCTTCCCTc                               >  2:556709/1‑101 (MQ=255)


                                                                         naTCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                             >  1:150464/2‑101 (MQ=255)


                                                                         gATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                             >  2:3207779/1‑101 (MQ=255)


                                                                           tCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                           <  1:1041999/101‑1 (MQ=255)


                                                                            cGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                          >  1:987302/1‑101 (MQ=255)


                                                                            cGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                          >  1:191017/1‑101 (MQ=255)


                                                                             gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                         >  1:3080522/1‑101 (MQ=255)


                                                                             gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                         >  1:1292857/1‑101 (MQ=255)


                                                                             gAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                         >  2:1564135/1‑101 (MQ=255)


                                                                              aaaCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                        <  1:170377/101‑1 (MQ=255)


                                                                              aaaCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                        >  2:4522897/1‑101 (MQ=255)


                                                                              aaaCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                        >  1:1252000/1‑101 (MQ=255)


                                                                                aCGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                      <  2:2518832/101‑1 (MQ=255)


                                                                                  gATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc                    <  1:4312263/101‑1 (MQ=255)


                                                                                   aTGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgct                   >  2:4951606/1‑100 (MQ=255)


                                                                                   aTGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgcg                   >  2:523347/1‑101 (MQ=255)


                                                                                    tGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGt                  <  1:228689/101‑1 (MQ=255)


                                                                                         gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTTCCGCGTCGc               >  2:2833145/1‑101 (MQ=255)


                                                                                         gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc               <  1:4495840/101‑1 (MQ=255)


                                                                                         gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc               >  2:2594215/1‑101 (MQ=255)


                                                                                          ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCt              <  2:2017453/101‑1 (MQ=255)


                                                                                           tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc             >  2:3013636/1‑101 (MQ=255)


                                                                                             aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccc           >  2:22764/1‑101 (MQ=255)


                                                                                              tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccc          <  1:2990033/101‑1 (MQ=255)


                                                                                              tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccc          >  1:3404837/1‑101 (MQ=255)


                                                                                               ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc         >  1:2956853/1‑101 (MQ=255)


                                                                                               ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc         >  1:3504857/1‑101 (MQ=255)


                                                                                               ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc         <  2:4269668/101‑1 (MQ=255)


                                                                                               ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccccc         <  2:4211254/101‑1 (MQ=255)


                                                                                                gCCTACGTCGGGCAGGGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccccc        <  2:3459970/101‑1 (MQ=255)


                                                                                                gCCTACGGCGGGCAGTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcccccc        <  1:1412277/101‑1 (MQ=255)


                                                                                                  cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgc      <  2:19189/101‑1 (MQ=255)


                                                                                                  cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgc      <  1:974746/101‑1 (MQ=255)


                                                                                                   tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgcg     >  2:4411338/1‑101 (MQ=255)


                                                                                                   tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgcg     >  1:661131/1‑101 (MQ=255)


                                                                                                    aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCCCCCCCCGCgg    <  1:4367038/101‑1 (MQ=255)


                                                                                                      gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgggg  >  1:2618753/1‑101 (MQ=255)


GGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGC..GTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395849‑1396049

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG