Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,134 T→G 2.5% S6A (TCG→GCC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1340T→G 2.5% 9.0 ‑1.3 ‑0.1274S6A (TCG→GCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (140/127):  total (142/132)
Fisher's exact test strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.51e-01

Only 100 of 299 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCT  >  NC_014034/1396034‑1396234
                                                                                                    |                                                                                                       
cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtttt                                                                                                         >  2:4120198/1‑101 (MQ=255)
cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtttt                                                                                                         >  1:1379457/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTc                                                                                                        >  1:4391948/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTc                                                                                                        >  2:2186054/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTc                                                                                                        >  2:27099/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCg                                                                                                       >  2:3608007/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGt                                                                                                      >  1:1108676/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTc                                                                                                     >  1:3314764/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCt                                                                                                <  2:4701808/101‑1 (MQ=255)
         cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                               <  2:2887261/101‑1 (MQ=255)
            gggggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.Ca                                                                                           >  1:911242/1‑101 (MQ=255)
                gCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCt                                                                                       <  1:823097/101‑1 (MQ=255)
                 cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                      >  2:3674369/1‑101 (MQ=255)
                 cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                      <  1:281683/101‑1 (MQ=255)
                  gTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcg                                                                                     >  2:298676/1‑101 (MQ=255)
                   tGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTccc                                                                                    >  1:3710408/1‑99 (MQ=255)
                    ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                   >  2:3650320/1‑101 (MQ=255)
                    ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                   >  1:3754818/1‑101 (MQ=255)
                      aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                 <  2:150239/101‑1 (MQ=255)
                      aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                 <  2:88173/101‑1 (MQ=255)
                        cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                               >  1:3810150/1‑101 (MQ=255)
                         gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                              >  2:1126209/1‑101 (MQ=255)
                         gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                              >  1:1000279/1‑101 (MQ=255)
                         gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCgccgcc                                                                              >  2:1645187/1‑101 (MQ=255)
                          aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAANTTTTCGTC.ATCTGG.CAGGCTCGCgcccccc                                                                             >  1:1751400/1‑100 (MQ=255)
                             cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCCCACGc                                                                          >  2:4155520/1‑101 (MQ=255)
                               ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTg                                                                        <  2:3933573/101‑1 (MQ=255)
                                gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGc                                                                       >  2:4833388/1‑101 (MQ=255)
                                 cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTggg                                                                      >  2:767283/1‑99 (MQ=255)
                                 cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCg                                                                      <  2:1911183/101‑1 (MQ=255)
                                  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGt                                                                     >  1:420886/1‑101 (MQ=255)
                                  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGt                                                                     >  1:277148/1‑101 (MQ=255)
                                  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGt                                                                     <  1:1689852/101‑1 (MQ=255)
                                   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCCCTGCGTc                                                                    >  2:4518433/1‑101 (MQ=255)
                                   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                    >  2:605772/1‑101 (MQ=255)
                                   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                    >  1:3977461/1‑101 (MQ=255)
                                      cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGCGGGTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCCGCGCCGcc                                                                 >  1:1811891/1‑100 (MQ=38)
                                           gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                            <  1:3399894/101‑1 (MQ=255)
                                            tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                           <  1:2210708/101‑1 (MQ=255)
                                             atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcg                                                          <  1:4675607/101‑1 (MQ=255)
                                              tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACGCTGCGTGGCCCCCCCcgc                                                         >  2:838740/1‑101 (MQ=255)
                                                tGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgg                                                       >  1:2013274/1‑101 (MQ=255)
                                                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                     <  2:2479700/101‑1 (MQ=255)
                                                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGATGCGTCGCTCCCCCCCCgggg                                                     >  2:543067/1‑101 (MQ=255)
                                                   ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCggggg                                                    >  2:3719429/1‑101 (MQ=255)
                                                       tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                >  1:3125486/11‑101 (MQ=9)
                                                       tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                <  2:10630/91‑1 (MQ=9)
                                                        gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                               >  1:1172676/10‑101 (MQ=2)
                                                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:4299983/101‑1 (MQ=255)
                                                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCCCGGGGGCGGgg                                               >  1:4461839/1‑101 (MQ=255)
                                                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGgg                                               >  1:734142/1‑101 (MQ=255)
                                                         agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTggg                                              >  1:3178220/1‑100 (MQ=255)
                                                         agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGa                                              >  1:3883656/1‑101 (MQ=255)
                                                           aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:1218781/101‑1 (MQ=255)
                                                            cGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  2:5046488/1‑101 (MQ=255)
                                                               cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aaa                                       <  1:27109/101‑1 (MQ=255)
                                                               cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aaa                                       >  1:2924918/1‑101 (MQ=255)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAc                                      <  2:1751270/101‑1 (MQ=255)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGGTCG.AAAc                                      >  1:962758/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAcc                                     >  2:3016838/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGCGGGCGCGGAACG.AAAcc                                     >  2:2469481/1‑101 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAccc                                    <  2:232097/101‑1 (MQ=255)
                                                                     cacccaccGCGGGGGCGTGGCTCGAATATTTTCATC.ATCAGG.CAGGCTAGCGCCGCCACGCTGCGTCGCGCCCCCCGCGGGGGCGTTGATCG.AAACCCGGc                                 <  1:2755345/99‑1 (MQ=25)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGGTGGCTGCCTCCCCCCGCGGGGGGGGGGACCA.TAACCCAGc                                 >  2:4578890/1‑101 (MQ=21)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCg                                >  1:2325966/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.GAGGCTCGCGACGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                               <  1:5027604/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGGGCG.AAACCCGGCGaaa                             >  1:3850887/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                             <  2:4391629/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                            <  1:4792169/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                            <  2:3518471/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGGGGATCG.AAACCCGGCGaaaa                            >  2:3420471/1‑101 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaaa                           <  1:3650592/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAACCCGGccacca                            >  2:2375979/1‑96 (MQ=38)
                                                                            cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAAc                          <  1:250222/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACg                         >  1:408899/1‑101 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACg                         <  2:4976597/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                       >  1:3019027/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                       <  2:4460290/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCa                     <  1:4423984/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCa                     <  1:1340406/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                   <  1:4155837/101‑1 (MQ=255)
                                                                                   gCGGGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                   <  1:556978/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                  <  2:4245240/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCAACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGcc                 >  2:2093416/1‑101 (MQ=255)
                                                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGccc                >  1:3251092/1‑100 (MQ=255)
                                                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                <  2:2767448/101‑1 (MQ=255)
                                                                                       ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCCTGCCTc               >  2:3742562/1‑101 (MQ=255)
                                                                                       ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTc               >  2:3184404/1‑101 (MQ=255)
                                                                                       ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAACCCGGCGAAAAAAGGCCATGCCTc               >  2:719338/1‑101 (MQ=255)
                                                                                        gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc              <  1:4481155/101‑1 (MQ=255)
                                                                                            gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgggg          >  2:2053838/1‑100 (MQ=255)
                                                                                            gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCcggg          >  2:4880280/1‑100 (MQ=255)
                                                                                             aaaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgtg         >  1:340984/1‑101 (MQ=255)
                                                                                              aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt        <  1:605851/101‑1 (MQ=255)
                                                                                               aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc       >  2:2559239/1‑101 (MQ=255)
                                                                                                cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTca      <  2:1811758/101‑2 (MQ=255)
                                                                                                  tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg    >  1:103811/1‑101 (MQ=255)
                                                                                                  tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg    >  2:3075802/1‑101 (MQ=255)
                                                                                                  tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg    <  1:2623050/101‑1 (MQ=255)
                                                                                                   ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc   >  2:3264667/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
CGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCT  >  NC_014034/1396034‑1396234

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG