Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,826 G→C 13.1% R236R (CGG→CTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8260G→C13.1% 98.3 ‑2.6 ‑0.0328R236R (CGG→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/29):  ref base (136/149):  total (150/178)
Fisher's exact test strand distribution p-value = 7.16e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.56e-01

Only 100 of 354 total aligned reads displayed.

GTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396726‑1396926
                                                                                                        |                                                                                                    
 tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGa                                                                                                     >  2:3144526/1‑101 (MQ=255)
  ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGAc                                                                                                    >  2:3671332/1‑101 (MQ=255)
   gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACg                                                                                                   >  2:3264616/1‑101 (MQ=255)
    aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                                                  >  1:4299818/1‑101 (MQ=255)
    aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                                                  <  1:3289239/101‑1 (MQ=255)
      cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                                                >  1:4842360/1‑101 (MQ=255)
      cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                                                <  2:4446964/101‑1 (MQ=255)
      cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGAGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                                                >  2:3890347/1‑101 (MQ=255)
       gAACCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                               <  2:712265/101‑1 (MQ=255)
       gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                               >  2:4976359/1‑101 (MQ=255)
       gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                               >  2:1311733/1‑101 (MQ=255)
       gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                               >  1:3336579/1‑101 (MQ=255)
             cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                                         >  1:3217629/1‑101 (MQ=255)
              tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGc                                                                                        >  2:2349914/1‑101 (MQ=255)
               gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                                       >  1:2894461/1‑101 (MQ=255)
                tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                                      >  1:4375200/1‑101 (MQ=255)
                 cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                                     >  1:1980164/1‑101 (MQ=255)
                 cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                                     <  1:875207/101‑1 (MQ=255)
                  ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                                    >  1:343902/1‑101 (MQ=255)
                   cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                                   <  1:1371765/101‑1 (MQ=255)
                    tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                                  <  2:4080747/101‑1 (MQ=255)
                     cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                                                 >  2:2837467/1‑101 (MQ=255)
                       tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                               >  1:3043155/1‑101 (MQ=255)
                       tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                               <  1:1053093/101‑1 (MQ=255)
                        atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgcg                                                                              >  1:1809880/1‑101 (MQ=255)
                          gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                                            >  1:3367914/1‑101 (MQ=255)
                             gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                         <  1:2802662/101‑1 (MQ=255)
                             gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                         <  2:2603580/101‑1 (MQ=255)
                              gcccGATGCCCTTGAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                        <  1:1248683/98‑1 (MQ=255)
                                  gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  1:1486199/101‑1 (MQ=255)
                                   aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:3565739/1‑101 (MQ=255)
                                   aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:4073518/101‑1 (MQ=255)
                                   aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  1:3983724/1‑101 (MQ=255)
                                     gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:257525/101‑1 (MQ=255)
                                     acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCNCCCGCGGGGGCGGNGAt                                                                   >  2:2165370/9‑101 (MQ=2)
                                      catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:3628620/94‑1 (MQ=2)
                                      catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:1839565/94‑1 (MQ=2)
                                      catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:2794331/94‑1 (MQ=2)
                                      catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:2931900/94‑1 (MQ=2)
                                      caccgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:4808016/94‑1 (MQ=2)
                                       atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  2:904771/7‑101 (MQ=2)
                                       atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:3877789/95‑1 (MQ=2)
                                        cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  1:4072030/1‑101 (MQ=255)
                                         ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  1:4676019/101‑1 (MQ=255)
                                          tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                            <  1:4757422/101‑1 (MQ=255)
                                          tGAGCCGCTCCCCCCGCGGGGGCGTGTACCGAAACCGTG.TGGAT...GATCATGCAGCCCGCACGGCTTGGGGCCCTCCCCCCGCGGGGGCGTGGGTCgcaccc                                                            >  2:549432/1‑96 (MQ=25)
                                          gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  1:1603431/98‑1 (MQ=2)
                                           ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  1:1079896/99‑1 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcat                                                           >  2:4710146/1‑99 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                                         <  2:4354938/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                          <  1:2430041/101‑4 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                        <  2:1764703/101‑1 (MQ=255)
                                               cGCGCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACCGGTcc                                                       <  2:3122956/101‑1 (MQ=255)
                                               cGCCCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                       <  1:3966475/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                      <  1:1136813/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                                     <  2:1462202/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                    >  2:4598267/1‑101 (MQ=255)
                                                  cccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                                      <  2:2408863/100‑8 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                                     >  2:79956/1‑93 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                   >  2:2666234/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                                  <  1:2844565/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG.TGCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTcgaagac                                                  <  2:1495138/101‑7 (MQ=2)
                                                      cccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                <  2:2032917/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                <  1:3671610/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                               <  2:2888386/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                               <  1:4724081/101‑1 (MQ=255)
                                                        cgcgGGGTCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                              <  1:3812370/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                             <  1:2112734/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                             <  1:4932497/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                            <  1:4570275/101‑1 (MQ=255)
                                                            gggcCGTAGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                          <  2:1206110/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                        <  2:3792281/101‑1 (MQ=255)
                                                                cGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                      >  1:813564/1‑101 (MQ=255)
                                                                cGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                      >  1:624168/1‑101 (MQ=255)
                                                                  gggATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                    <  2:4159182/100‑1 (MQ=255)
                                                                    gATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                  <  2:611950/101‑1 (MQ=255)
                                                                    gATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                  >  1:2186120/1‑101 (MQ=255)
                                                                     aTCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                                 >  1:2059345/1‑101 (MQ=255)
                                                                       cGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGACACCGGTCCAGAGATAGGCCGCGGGGAcctcct                               >  2:3985007/1‑101 (MQ=255)
                                                                        gAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACCCGTCCATAACTAGACGGCGCGGCCctcctc                              >  2:3355469/1‑101 (MQ=37)
                                                                        gAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                              <  2:1121665/101‑1 (MQ=255)
                                                                         aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                             <  2:3015489/101‑1 (MQ=255)
                                                                           aCCGTG.TGGAT...GAGCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                           <  2:1667616/101‑1 (MQ=255)
                                                                            ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                          <  2:1555624/101‑1 (MQ=255)
                                                                             cGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                         <  1:2690172/101‑1 (MQ=255)
                                                                              cttgtgcgt...gatCATTCTCCGTGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCCGGTCCAGAGATAGACCGCGCGGACCGCCTCAGACg                         <  2:3479123/93‑1 (MQ=25)
                                                                                g.tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                      >  2:1391881/1‑101 (MQ=255)
                                                                                   ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt                    >  1:1671224/1‑101 (MQ=255)
                                                                                   ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt                    >  2:2436145/1‑101 (MQ=255)
                                                                                    gat...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                   <  1:3114995/101‑1 (MQ=255)
                                                                                     gt...gatCATGGAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGCGGATCGAACCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc                  <  1:549511/100‑1 (MQ=255)
                                                                                      t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                 <  1:1201339/101‑1 (MQ=255)
                                                                                      t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                 >  1:1512595/1‑101 (MQ=255)
                                                                                           atCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc               >  2:1845049/1‑101 (MQ=255)
                                                                                            tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc              <  1:3566009/101‑1 (MQ=255)
                                                                                                 cAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACCGGGCCAGAGATAGACCGCGCGGACCTCCCCCGACGCTGCACCCCCCGCggg         >  2:2502135/1‑101 (MQ=25)
                                                                                                  aGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg        >  1:4168925/1‑101 (MQ=255)
                                                                                                   gcgcGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg       <  1:4617911/101‑1 (MQ=255)
                                                                                                   gcgcGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggggg       <  1:4106466/101‑1 (MQ=255)
                                                                                                     gcgGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCg     >  1:228463/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
GTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396726‑1396926

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG