Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,938 C→T 11.7% M274M (CTG→TGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9380C→T11.7% 120.2 ‑0.1 ‑0.0427M274M (CTG→TTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/23):  ref base (181/160):  total (218/209)
Fisher's exact test strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.04e-01

Only 100 of 458 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGC  >  NC_014034/1396839‑1397038
                                                                                                     |                                                                                                                  
gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCt                                                                                                                   <  2:4744704/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.g                                                                                                                 <  1:1041585/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.g                                                                                                                 <  2:813462/101‑1 (MQ=255)
   ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.gcg                                                                                                               >  2:2389414/1‑101 (MQ=255)
     ccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.Gcgcg                                                                                                             >  2:2689312/1‑101 (MQ=255)
       ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAC.A.GCCT.GCGCG.G..c                                                                                                        >  2:300366/1‑101 (MQ=255)
          cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGc                                                                                                    >  2:2195016/1‑101 (MQ=255)
           gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.a                                                                                                  >  1:703835/1‑101 (MQ=255)
           gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.a                                                                                                  >  1:2959220/1‑101 (MQ=255)
            ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..t                                                                                               <  1:4668657/101‑1 (MQ=255)
             gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..Tc                                                                                              <  2:1724295/101‑1 (MQ=255)
                cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGc                                                                                           >  2:439317/1‑101 (MQ=255)
                 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCt                                                                                          <  1:2436319/101‑1 (MQ=255)
                 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGAGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCt                                                                                          >  1:3517755/1‑101 (MQ=255)
                  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCt.t                                                                                        >  2:958840/1‑101 (MQ=255)
                  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCt.t                                                                                        >  1:4414915/1‑101 (MQ=255)
                     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCg                                                                                     >  2:4124382/1‑101 (MQ=255)
                       cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGc.c                                                                                  >  1:4975406/1‑101 (MQ=255)
                          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..c                                                                             >  1:4145564/1‑101 (MQ=255)
                          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCCC.CCG..c                                                                             >  1:3920367/1‑101 (MQ=255)
                              ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CCG..CG.Tg.g                                                                       >  2:1399264/1‑101 (MQ=255)
                               gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CCG..CG.TG.Gt                                                                      >  1:2327877/1‑101 (MQ=255)
                                   agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCt                                                                  >  1:1299497/1‑101 (MQ=255)
                                     agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTcc                                                                >  2:1233636/1‑101 (MQ=255)
                                     agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTcc                                                                >  2:2929718/1‑101 (MQ=255)
                                      gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CCG..CG.Gg.ggccccccc                                                               >  1:3424814/1‑93 (MQ=37)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTcccccc                                                            <  2:5028414/101‑1 (MQ=255)
                                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTcccccc                                                            >  2:3407247/1‑101 (MQ=255)
                                          gACCGCGCGGACCTCCTCAGACGTCGCTCCCGCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCcg                                                           >  1:4485513/1‑101 (MQ=255)
                                          gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GGCGCTCCCCCcg                                                           >  2:4440185/1‑101 (MQ=255)
                                           aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCcgc                                                          >  1:196578/1‑101 (MQ=255)
                                                   gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGt                                                  <  2:3633955/99‑1 (MQ=255)
                                                   gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGt                                                  <  2:1809747/101‑1 (MQ=255)
                                                    aCCTCCCCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTg                                                 <  2:3189345/101‑1 (MQ=255)
                                                      tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:1455824/92‑1 (MQ=0)
                                                      ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:3587270/91‑1 (MQ=9)
                                                      ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                               <  1:2808515/101‑1 (MQ=255)
                                                       tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCCTgggt                                              >  1:4765541/1‑99 (MQ=255)
                                                        tggccccgggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:29207/91‑1 (MQ=2)
                                                        tcaccactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:1344756/93‑1 (MQ=9)
                                                        cctcgcaCGTCGGCCCCCCCGCCGGGGCGTGGATGGAAGCAA.TGCG.GCGCG.G..G.CCC.A..T.GGCT.GCCGG.ACC..CGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:4801567/95‑1 (MQ=0)
                                                        cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  2:2606539/1‑101 (MQ=255)
                                                        cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:579960/101‑1 (MQ=255)
                                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:2031600/8‑101 (MQ=2)
                                                         ctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                            <  2:3920075/101‑1 (MQ=255)
                                                         cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:4939102/95‑1 (MQ=2)
                                                         cggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:1431807/95‑1 (MQ=2)
                                                          ggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  2:1666920/96‑1 (MQ=2)
                                                          aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:4290267/7‑101 (MQ=9)
                                                           ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:4591282/96‑1 (MQ=2)
                                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:1845182/101‑1 (MQ=255)
                                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                          >  1:635910/1‑101 (MQ=255)
                                                           acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  1:4947849/6‑101 (MQ=9)
                                                            gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  2:4175570/4‑101 (MQ=2)
                                                            cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:903933/97‑1 (MQ=2)
                                                             actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  1:1898214/4‑101 (MQ=9)
                                                              ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        <  2:230965/99‑1 (MQ=9)
                                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                       >  1:1780662/2‑100 (MQ=9)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:2838087/101‑1 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                      <  2:1090364/101‑3 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATc                                     >  2:1004889/1‑101 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                     >  1:3824646/1‑98 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActc                                      >  1:1664245/1‑99 (MQ=9)
                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                     <  1:2606720/101‑1 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                    <  1:2667094/101‑1 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                    >  1:4860951/1‑101 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCACCGCGGGGGCGTGGATCGAAActaca                                    >  1:4238981/1‑97 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                   >  2:1351571/1‑101 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAActaaac                                   >  2:4009691/1‑96 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                    >  1:3678483/1‑97 (MQ=9)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                    >  1:2176174/1‑97 (MQ=9)
                                                                  cGCCCCCCCCGCGGGGGCGTGGATCGAAAC.T.TGAT.GCCCA.GATC.CGCAA..TCGGC...CGGG.CTC..GA.TC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcctat                                    <  2:2876833/101‑5 (MQ=0)
                                                                   ccTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                     <  2:3235949/100‑3 (MQ=2)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                    <  2:2053069/101‑1 (MQ=2)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCTCCGCGGGGGCGTGGATCGAAACATCAGCt                                 <  2:468931/101‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCATCt                                 >  2:1133706/1‑101 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                >  1:322135/1‑93 (MQ=2)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAActcggcct                                 >  1:4080572/1‑94 (MQ=9)
                                                                     cccccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                   <  1:223403/100‑2 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                  <  1:1519451/101‑3 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                  <  1:313218/101‑3 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGaaaatacaccggg                               >  2:1955271/1‑91 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                               >  1:1484403/1‑92 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.A..ACT.GACC.....C.GGC.A..ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                               >  2:3062559/1‑92 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCG..C.CGC.G..CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAActcggcctc                                >  2:664684/1‑93 (MQ=9)
                                                                       cccccGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.G..C.AGT.G..TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgc                               >  1:2290327/1‑92 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..C.ATG.AGTTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                <  2:4299223/101‑5 (MQ=0)
                                                                        ccccGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                             <  2:1537314/101‑1 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                              <  1:1141240/101‑7 (MQ=0)
                                                                           cgcgGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.C..CATGA.AT.TCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                             <  2:2810741/101‑8 (MQ=0)
                                                                           cgcgGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                          <  1:3333269/101‑1 (MQ=255)
                                                                                  gCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                   <  2:3336316/101‑1 (MQ=255)
                                                                                   ggTGGATAGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                  <  2:747199/100‑1 (MQ=255)
                                                                                    gTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTggacgccga                 >  1:2120748/1‑94 (MQ=255)
                                                                                    gTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                 <  1:3623409/101‑1 (MQ=255)
                                                                                      ggATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa               <  2:3755266/101‑1 (MQ=255)
                                                                                      ggATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa               >  1:4006472/1‑101 (MQ=255)
                                                                                        aTCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg             >  2:2654078/1‑101 (MQ=255)
                                                                                           gAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg          <  1:771636/101‑1 (MQ=255)
                                                                                              aC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc       >  1:377430/1‑101 (MQ=255)
                                                                                                     |                                                                                                                  
GCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.G..C.CGC.A..TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGC  >  NC_014034/1396839‑1397038

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG