Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,941 C→A 11.9% R275S (CGC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9410C→A11.9% 106.0 ‑0.9 ‑0.0407R275S (CGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (26/19):  ref base (167/165):  total (208/199)
Fisher's exact test strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.16e-01

Only 100 of 451 total aligned reads displayed.

TCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCC  >  NC_014034/1396841‑1397041
                                                                                                       |                                                                                                             
 ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.gcg                                                                                                              <  2:3344597/101‑1 (MQ=255)
  cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.gcgc                                                                                                             <  2:1684098/101‑1 (MQ=255)
             gCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCgg                                                                                               >  1:658246/1‑101 (MQ=255)
              cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                              >  1:2307705/1‑101 (MQ=255)
               gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                             <  1:2436319/101‑1 (MQ=255)
               gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGAGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                             >  1:3517755/1‑101 (MQ=255)
                tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                           <  1:1484668/101‑1 (MQ=255)
                tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                           >  2:958840/1‑101 (MQ=255)
                  gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.Tcc                                                                                         <  1:1788990/101‑1 (MQ=255)
                  gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.Tca                                                                                         >  1:3671096/1‑100 (MQ=255)
                   aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                        >  2:4124382/1‑101 (MQ=255)
                     cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCACCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCG.TCCGc.c                                                                                     >  2:1467948/1‑101 (MQ=255)
                     cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                     <  1:3593500/101‑1 (MQ=255)
                      gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                    <  2:658157/101‑1 (MQ=255)
                      gAAACCGGCGCAGAGATAGACCGCGCGTGCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                    <  2:3774023/101‑1 (MQ=38)
                            ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.Tg.g                                                                          >  1:468996/1‑101 (MQ=255)
                                 agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCt                                                                     >  2:4417943/1‑101 (MQ=255)
                                  gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.CG.GTCGCTc                                                                    >  1:722720/1‑101 (MQ=255)
                                    gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GGCGCTccc                                                                  >  2:1724329/1‑101 (MQ=255)
                                    gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CCG..CG.Gg.ggccccccc                                                                  >  1:3424814/1‑93 (MQ=37)
                                     aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTcccc                                                                 >  2:1439239/1‑101 (MQ=255)
                                     aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTC..CG.TG.GGCGCTcccc                                                                 >  2:1226999/1‑101 (MQ=255)
                                          ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCcgcg                                                            >  2:4995430/1‑101 (MQ=255)
                                            gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCggg                                                          >  2:1112714/1‑101 (MQ=255)
                                               cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTC..CG.TG.GTCGCTCCCCCCGCGGGGGc                                                       >  1:4994181/1‑101 (MQ=255)
                                               cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTC..CG.TG.GTCGCTCCCCCCGCGGGGGc                                                       >  1:2130045/1‑101 (MQ=255)
                                                ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCg                                                      >  2:2226839/1‑101 (MQ=255)
                                                 gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCgg                                                     >  2:2315217/1‑100 (MQ=255)
                                                    tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:4033074/92‑1 (MQ=0)
                                                    ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTgg                                                   >  1:4002526/11‑101 (MQ=9)
                                                    ctcctcAGACGTCTCGCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  2:2635324/101‑1 (MQ=255)
                                                    ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  1:2808515/101‑1 (MQ=255)
                                                    attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  2:743935/91‑1 (MQ=0)
                                                    attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  2:3242318/11‑101 (MQ=0)
                                                      tggccccgggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:29207/91‑1 (MQ=2)
                                                      cctcgcaCGTCGGCCCCCCCGCCGGGGCGTGGATGGAAGCAA.TGCG.GCGCG.GG.CCCA.T.GGCT.GCCGG.ACC..CGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  2:4801567/95‑1 (MQ=0)
                                                      cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCCCCCCCCGCGGGGGCGTGGATc                                                <  1:2874552/101‑1 (MQ=255)
                                                       ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:1054391/101‑1 (MQ=255)
                                                       ctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                               <  2:3920075/101‑1 (MQ=255)
                                                        tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  2:1290338/101‑1 (MQ=255)
                                                        ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:2755801/96‑1 (MQ=2)
                                                        ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGGGGACCg                                               >  2:2397628/6‑101 (MQ=0)
                                                        gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  2:3462617/7‑101 (MQ=2)
                                                        aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCCCCCCCCGCGGGGGGGGGGGTCg                                               >  1:3902051/7‑101 (MQ=2)
                                                         cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:1845182/101‑1 (MQ=255)
                                                         acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:1521890/6‑101 (MQ=9)
                                                          cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:4529255/97‑1 (MQ=9)
                                                          aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGGGGGGGTCGaaa                                            >  1:20549/1‑101 (MQ=255)
                                                          aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            >  2:185027/1‑101 (MQ=255)
                                                           gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:3771865/101‑1 (MQ=255)
                                                            aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                          <  2:3589981/101‑1 (MQ=255)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:440269/1‑101 (MQ=2)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:412049/1‑101 (MQ=2)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                         >  1:4469845/1‑99 (MQ=2)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                         >  1:1057255/1‑99 (MQ=9)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActc                                         >  1:1664245/1‑99 (MQ=9)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                         >  1:1221739/1‑100 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCC.CCGGATCTTTGT.CTG..CG.CG.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                       >  1:2235468/1‑97 (MQ=9)
                                                                 gCTCCCCCCGCGGGGGCGTGGATTGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGATCCCCCCGCGGGGGGGTGGTTCGAAACATAAGc                                     >  1:4064415/1‑101 (MQ=37)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.TCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                      <  1:4247461/101‑6 (MQ=9)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                      >  1:1927665/1‑96 (MQ=9)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATGGAAActcggcc                                     >  2:319463/1‑95 (MQ=9)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAAC.ATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                                    >  1:483853/1‑94 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                   >  1:322135/1‑93 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                    <  2:4739187/101‑8 (MQ=9)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCTCCCGCGGGGGCGTGGATCGAAACACCaat                                     >  2:3077635/1‑99 (MQ=2)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                     <  1:313218/101‑3 (MQ=2)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCGTGGGTCGAAACATCAGCtcg                                  >  2:1958367/1‑101 (MQ=255)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC.A..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                  <  2:4469526/101‑10 (MQ=2)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                   <  1:3033146/101‑9 (MQ=9)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                   >  2:3037009/1‑93 (MQ=9)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                   <  1:970883/101‑9 (MQ=9)
                                                                    ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                   >  1:903897/1‑93 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgc                                 >  2:2934152/1‑100 (MQ=255)
                                                                     cccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                  <  1:1301550/101‑10 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                  <  2:4921676/101‑10 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                  <  2:1579696/101‑10 (MQ=9)
                                                                       cccGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                               <  2:2885350/101‑1 (MQ=255)
                                                                       cccGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                               <  1:634202/101‑1 (MQ=255)
                                                                        ccGCGGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                 <  2:1387004/101‑7 (MQ=0)
                                                                         cgcgGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                <  2:2810741/101‑8 (MQ=0)
                                                                         cgcgGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                <  1:4192660/101‑8 (MQ=0)
                                                                         cgcgGGGGCGTGGATCGAAAC.T.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                <  2:3670359/101‑8 (MQ=0)
                                                                         cgcgGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                             <  1:3333269/101‑1 (MQ=255)
                                                                         cgcgGGGGCGTGGAGCGAACC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                             <  2:799502/101‑1 (MQ=255)
                                                                          gggggggCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CCCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                            <  1:2195171/99‑1 (MQ=255)
                                                                          gcgGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGATg                            >  2:4255975/1‑101 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                           <  1:2689497/101‑1 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.GG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGTCGTTgg                           >  1:3792349/1‑101 (MQ=255)
                                                                            gggggCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                          <  2:4675463/101‑1 (MQ=255)
                                                                                  gTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                    >  1:1970120/1‑101 (MQ=255)
                                                                                      aTCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                <  1:2350078/101‑1 (MQ=255)
                                                                                      aTCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                >  2:2654078/1‑101 (MQ=255)
                                                                                        cGAAAC.A.GCCG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt              <  2:2069792/101‑1 (MQ=255)
                                                                                          aaaC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt            >  2:4302216/1‑101 (MQ=255)
                                                                                          aaaC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt            >  1:1359769/1‑101 (MQ=255)
                                                                                          aaaC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt            >  1:4963719/1‑101 (MQ=255)
                                                                                            aC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc          <  1:1439366/101‑1 (MQ=255)
                                                                                             c.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg         <  1:2595785/101‑1 (MQ=255)
                                                                                             c.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg         >  1:4876284/1‑101 (MQ=255)
                                                                                                       |                                                                                                             
TCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCC  >  NC_014034/1396841‑1397041

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG