BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,955	C→A	12.2%	G279G (GGC→ATA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,955	0	C→A	12.2%	101.8 ‑0.1 0.0	410	G279G (GGC→GGA)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (24/23): ref base (164/168): total (203/207)
Fisher's exact test strand distribution p-value = 8.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 447 total aligned reads displayed.


CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055


cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGc                                                                                                             >  2:439317/1‑101 (MQ=255)


cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGc                                                                                                             >  2:3322895/1‑101 (MQ=255)


  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                          >  2:958840/1‑101 (MQ=255)


  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                          <  1:1484668/101‑1 (MQ=255)


     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                                       >  2:4124382/1‑101 (MQ=255)


        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGGCGCTCCTCCCGCGGGGACGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                   >  2:1654952/1‑101 (MQ=255)


         aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..g                                                                                                >  2:1988327/1‑101 (MQ=255)


          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..c                                                                                             >  1:4145564/1‑101 (MQ=255)


          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..c                                                                                             <  1:2135722/101‑1 (MQ=255)


          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCCC.CC..G..c                                                                                             >  1:3920367/1‑101 (MQ=255)


           aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..Cg                                                                                            >  2:1096149/1‑101 (MQ=255)


               gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CC..G..CGTG.Gt                                                                                       >  1:2327877/1‑101 (MQ=255)


                 ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TGCGC.CC..G..CGTG.GTCg                                                                                     >  2:2844613/1‑101 (MQ=255)


                 ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCg                                                                                     >  2:2677452/1‑101 (MQ=255)


                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..C..CGTG.GGCGCTcccc                                                                               >  2:1226999/1‑101 (MQ=255)


                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTcccccc                                                                             >  2:3407247/1‑101 (MQ=255)


                               cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCgggg                                                                       <  1:1988471/101‑1 (MQ=255)


                                  ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                    >  2:1847137/1‑101 (MQ=255)


                                   gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGTTCCCCCCGCGGGGGCGt                                                                   <  1:1854325/99‑1 (MQ=255)


                                     cctcctCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CCCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:4993851/101‑1 (MQ=255)


                                      tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:1455824/92‑1 (MQ=0)


                                      ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  1:3474085/11‑101 (MQ=9)


                                      ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:3587270/91‑1 (MQ=9)


                                      ctcctcAGACGTCTCGCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  2:2635324/101‑1 (MQ=255)


                                       ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  1:4088813/10‑101 (MQ=2)


                                       tcctcAGGCGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:3091438/101‑1 (MQ=255)


                                       ccctcAGACGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGC.GGGGCGTGGATc                                                              <  2:41905/100‑1 (MQ=38)


                                        tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:3217404/93‑1 (MQ=2)


                                        cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:936471/101‑1 (MQ=255)


                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:3027319/94‑1 (MQ=2)


                                         ctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                                             <  2:3920075/101‑1 (MQ=255)


                                          tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:1290338/101‑1 (MQ=255)


                                          tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                            >  1:3802797/1‑101 (MQ=255)


                                          aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:4290267/7‑101 (MQ=9)


                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:3157727/101‑1 (MQ=255)


                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  1:3260658/1‑101 (MQ=255)


                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                           >  1:635910/1‑101 (MQ=255)


                                            aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGGGGGGGTCGaaa                                                          >  1:20549/1‑101 (MQ=255)


                                             tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:290430/3‑101 (MQ=2)


                                             cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  1:4788053/99‑1 (MQ=2)


                                              gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GG.CTATGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  1:716399/100‑1 (MQ=2)


                                              ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:230965/99‑1 (MQ=9)


                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        >  1:1780662/2‑100 (MQ=9)


                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:477790/1‑101 (MQ=2)


                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         >  1:440269/1‑101 (MQ=2)


                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATc                                                      >  2:1004889/1‑101 (MQ=255)


                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                       >  1:1057255/1‑99 (MQ=9)


                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActc                                                       >  1:1664245/1‑99 (MQ=9)


                                                gTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                      <  1:3648862/101‑4 (MQ=2)


                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCa                                                     >  1:3577883/1‑101 (MQ=255)


                                                 tCGCTCCACCCGCGGGGTCGTGGGCCGAAACA..ACGCG.GCGCG.GG.CCTA.T.GGCTGTCGCC.CC..C..CCCC.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAActgc                                                      <  2:710065/101‑4 (MQ=2)


                                                 gcGCGCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                     <  2:624089/100‑1 (MQ=255)


                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                       >  1:1221739/1‑100 (MQ=2)


                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                       <  2:1704308/101‑2 (MQ=2)


                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAActaaac                                                    >  2:4009691/1‑96 (MQ=2)


                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                   >  1:3393917/1‑95 (MQ=2)


                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                    >  1:1927665/1‑96 (MQ=9)


                                                   ccTCCCCCCGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                      <  2:3235949/100‑3 (MQ=2)


                                                   ccTCCCCCCGCGGGGGCGGGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCacc                                                   <  2:4909765/100‑3 (MQ=255)


                                                    ccccccccGCGGGGGCGTGGGTCCAAACATTGC.TT.TGGCG.GC.AGTG.TCAGGT.T.TTC.CA..G..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                   <  2:2766090/99‑7 (MQ=2)


                                                    cacccccgGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                                  <  1:4995760/99‑1 (MQ=255)


                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCTCCGCGGGGGCGTGGATCGAAACATCAGCt                                                  <  2:468931/101‑1 (MQ=255)


                                                    cTCCCCCCGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                   <  1:2835879/101‑7 (MQ=9)


                                                     cccccccGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                  <  1:4517929/100‑8 (MQ=9)


                                                      ccccccGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                   <  1:2645404/101‑3 (MQ=2)


                                                      ccccccGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                   <  1:1519451/101‑3 (MQ=2)


                                                      ccccccGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCTGGG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                >  1:4245610/1‑92 (MQ=2)


                                                      ccccccGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                >  2:3062559/1‑92 (MQ=2)


                                                      ccccccGCGGGGGCGTGGATCGAAACA...A.CT.GACC...C.GGCA.ACGGCC.TATGCGCT..GGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                <  2:4469526/101‑10 (MQ=2)


                                                       cccccGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                <  1:2414911/101‑10 (MQ=9)


                                                       cccccGCGGGGGCGTGGATCGAAAC...GC.TG.GCGCGCGC.CGCG.CCGGATCTTTGT.CT..G..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                <  1:1301550/101‑10 (MQ=9)


                                                          ccGCGGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                               <  1:2469531/101‑7 (MQ=0)


                                                          ccGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                            <  1:2943651/101‑1 (MQ=255)


                                                           cgcgGGGGCGTGGATCGAAACT..GC.GT.GCGCG.CCATGAATTCATCG.AGCGC.CA..T..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                              <  2:3670359/101‑8 (MQ=0)


                                                           cgcgGGGGCGTGGAGCGAACCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                           <  2:799502/101‑1 (MQ=255)


                                                            gggggggCGTGGATCGAAACA..GC.CT.GCGCG.GC.CCCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                          <  1:2195171/99‑1 (MQ=255)


                                                             cgGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                         <  1:1394581/101‑1 (MQ=255)


                                                              gggggCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                        >  1:4004196/1‑101 (MQ=255)


                                                               ggggGGTGGATCCAAACA..GC.CT.GCGCG.GC.CGCA.TCGGTT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                       <  1:4875229/101‑1 (MQ=255)


                                                                   gggggATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                   <  2:1504348/98‑1 (MQ=255)


                                                                      ggATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                >  2:1399203/1‑101 (MQ=255)


                                                                      ggATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                <  2:3755266/101‑1 (MQ=255)


                                                                        aTCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                              >  1:2718795/1‑101 (MQ=255)


                                                                           gAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                           <  1:771636/101‑1 (MQ=255)


                                                                            aaaCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                          >  2:4302216/1‑101 (MQ=255)


                                                                            aaaCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                          >  1:4963719/1‑101 (MQ=255)


                                                                              aCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                        <  1:1439366/101‑1 (MQ=255)


                                                                              aCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                        >  2:1913806/1‑101 (MQ=255)


                                                                              aCA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                        >  1:377430/1‑101 (MQ=255)


                                                                                    c.cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTcc                    >  2:2398575/1‑100 (MQ=255)


                                                                                    c.cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCg                    >  1:2236265/1‑101 (MQ=255)


                                                                                         gcgcG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                 >  2:1358561/1‑101 (MQ=255)


                                                                                             g.gC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc             >  2:1131640/1‑101 (MQ=255)


                                                                                               gC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc            <  1:2897020/101‑1 (MQ=255)


                                                                                                   gCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg         >  1:3733803/1‑101 (MQ=255)


                                                                                                   gCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg         <  1:4434090/101‑1 (MQ=255)


                                                                                                    cA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg        >  1:2300340/1‑101 (MQ=255)


                                                                                                      ctCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg       <  2:2185965/100‑1 (MQ=255)


                                                                                                        cGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg     >  1:4604600/1‑101 (MQ=255)


                                                                                                         ggCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc    >  2:4591499/1‑101 (MQ=255)


CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GC.CT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CT..G..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG