Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,960 G→T 7.5% R281L (CGC→TTT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9600G→T 7.5% 61.6 ‑0.2 ‑0.3399R281L (CGC→CTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/16):  ref base (180/188):  total (195/204)
Fisher's exact test strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.11e-01

Only 100 of 436 total aligned reads displayed.

ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060
                                                                                                             |                                                                                                         
  cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                                        >  1:2252519/1‑101 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGGCGCTCCTCCCGCGGGGACGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                                       >  2:1654952/1‑101 (MQ=255)
            ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TG.CGC.CCG..CG.TG.GTCg                                                                                          >  2:2844613/1‑101 (MQ=255)
               gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTc                                                                                       >  2:1116184/1‑101 (MQ=255)
                   tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CCC.CTG..CG.TG.GTCGCTccccc                                                                                   >  1:3955416/1‑101 (MQ=255)
                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTcccccc                                                                                  >  2:3407247/1‑101 (MQ=255)
                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTcccccc                                                                                  <  2:228429/101‑1 (MQ=255)
                     gACCGCGCGGACCTCCTCAGACGTCGCTCCCGCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcg                                                                                 >  1:4485513/1‑101 (MQ=255)
                      aCCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcgc                                                                                <  2:3948293/101‑1 (MQ=255)
                       ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcgcg                                                                               >  2:4995430/1‑101 (MQ=255)
                            cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTC..CG.TG.GTCGCTCCCCCCGCGGGGGc                                                                          >  1:4994181/1‑101 (MQ=255)
                             ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCg                                                                         >  2:1847137/1‑101 (MQ=255)
                                 tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  1:4696864/92‑1 (MQ=0)
                                 tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  2:4033074/92‑1 (MQ=0)
                                 ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  2:3587270/91‑1 (MQ=9)
                                 ctcctcAGACGTCTCGCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:2635324/101‑1 (MQ=255)
                                  tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:2447335/101‑1 (MQ=255)
                                  gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  2:4264088/10‑101 (MQ=9)
                                   tggccccgggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:29207/91‑1 (MQ=2)
                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  1:1945509/9‑101 (MQ=2)
                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  1:1501469/9‑101 (MQ=2)
                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:629030/93‑1 (MQ=2)
                                   cctcgcaCGTCGGCCCCCCCGCCGGGGCGTGGATGGAAGCAA..TGCG.GCGCG.GG.CCCA.T.GGCT.GC.CGG.ACC..CGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:4801567/95‑1 (MQ=0)
                                   cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCCCCCCCCGCGGGGGCGTGGATc                                                                   <  1:2874552/101‑1 (MQ=255)
                                    ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:3027319/94‑1 (MQ=2)
                                    ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:3141846/101‑1 (MQ=255)
                                    cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:4132904/95‑1 (MQ=2)
                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:951001/94‑1 (MQ=9)
                                     ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGGGGACCg                                                                  >  2:2397628/6‑101 (MQ=0)
                                     ccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:2218559/100‑1 (MQ=255)
                                     aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:4290267/7‑101 (MQ=9)
                                       cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  1:903933/97‑1 (MQ=2)
                                        tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  2:290430/3‑101 (MQ=2)
                                        actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  1:1898214/4‑101 (MQ=9)
                                         gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:716399/100‑1 (MQ=2)
                                         ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:312723/3‑101 (MQ=9)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            <  1:2001456/100‑3 (MQ=2)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            <  1:2707310/100‑3 (MQ=2)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  1:419238/1‑101 (MQ=2)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                            <  2:1090364/101‑3 (MQ=2)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATc                                                           >  2:1004889/1‑101 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                            >  1:1057255/1‑99 (MQ=9)
                                           gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                           <  1:2606720/101‑1 (MQ=255)
                                           gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..CCCT.GCGCC.GC.AGCG.AGTGCG.TCTGGA.ACA..GG.AT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            <  2:4434227/101‑3 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                             <  2:1261210/101‑1 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          <  1:1600159/101‑1 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          >  1:4860951/1‑101 (MQ=255)
                                            gcGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCa                                                          <  2:2129897/100‑1 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            >  1:1221739/1‑100 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  2:1704308/101‑2 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                                         >  2:1351571/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                          >  1:3678483/1‑97 (MQ=9)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                           >  1:4938516/1‑99 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                        <  1:2155961/101‑7 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.TCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                         <  1:4247461/101‑6 (MQ=9)
                                              ccTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                           <  2:3235949/100‑3 (MQ=2)
                                              ccTCCCCCCGCGGGGGCGGGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCacc                                                        <  2:4909765/100‑3 (MQ=255)
                                               ccccccccGCGGGGGCGTGGGTCCAAAC.ATTGCTT.TGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                        <  2:2766090/99‑7 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                          <  1:3411648/101‑1 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                          <  1:4542812/101‑1 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccccaa                                                         >  1:23676/1‑96 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       <  2:4739187/101‑8 (MQ=9)
                                                 ccccccGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccgccccg                                                      >  2:1446/1‑92 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAAC.AT.GGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                                      >  1:3586121/1‑93 (MQ=0)
                                                 ccccccGCGGGGGCGTGGATCGAAAC.A...ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                     >  2:3062559/1‑92 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAActcggcctc                                                      >  2:664684/1‑93 (MQ=9)
                                                 ccccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                      <  1:3033146/101‑9 (MQ=9)
                                                 ccccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                      >  1:2809388/1‑93 (MQ=9)
                                                  cccccGCGGGGGCGTGGATCGAAAC....GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                     <  1:1301550/101‑10 (MQ=9)
                                                      cgcgGGGGCGTGGATCGAAAC.T..GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                   <  2:3670359/101‑8 (MQ=0)
                                                      cgcgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                                <  1:3333269/101‑1 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                                <  1:210884/101‑1 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                               >  1:3748730/1‑101 (MQ=255)
                                                        cgGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                              <  1:1394581/101‑1 (MQ=255)
                                                        cgGGGGCGGGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                              <  1:2315381/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCAGCTCGTCGTTGGTc                                            >  2:4542878/1‑101 (MQ=255)
                                                          ggggCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCAGCTCGTCGTTGGTc                                            >  1:3835259/1‑101 (MQ=255)
                                                              gggggATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                        <  2:1504348/98‑1 (MQ=255)
                                                              ggTGGATAGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                        <  2:747199/100‑1 (MQ=255)
                                                              cGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                        <  1:3496113/101‑1 (MQ=255)
                                                                tGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                      <  2:3311936/101‑1 (MQ=255)
                                                                tGGATCAAAAC.A..GCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                      <  1:1791788/101‑1 (MQ=255)
                                                                 ggATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  1:3777720/1‑101 (MQ=255)
                                                                 ggATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     <  2:3755266/101‑1 (MQ=255)
                                                                 ggATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  1:4006472/1‑101 (MQ=255)
                                                                  gATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                    <  1:3229515/101‑1 (MQ=255)
                                                                   aTCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                   >  2:2654078/1‑101 (MQ=255)
                                                                      gAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                <  1:771636/101‑1 (MQ=255)
                                                                      gAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                >  2:4320729/1‑101 (MQ=255)
                                                                        aaC.A..GCCT.GGGCG.GC.CTCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                              <  1:1468078/101‑1 (MQ=255)
                                                                        aaC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                              <  1:1694266/101‑1 (MQ=255)
                                                                         aC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                             >  2:1913806/1‑101 (MQ=255)
                                                                                 cT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                        >  1:4091246/1‑101 (MQ=255)
                                                                                        g.gC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                  >  2:121791/1‑101 (MQ=255)
                                                                                              gCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              >  2:4655587/1‑101 (MQ=255)
                                                                                              gCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              >  2:1396510/1‑101 (MQ=255)
                                                                                              gCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              <  1:4434090/101‑1 (MQ=255)
                                                                                                a.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg            >  2:56684/1‑101 (MQ=255)
                                                                                                    ggCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGAGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc         <  1:4368236/101‑1 (MQ=255)
                                                                                                         tC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTgg     <  2:1969976/101‑1 (MQ=255)
                                                                                                             |                                                                                                         
ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG