Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,967 T→C 17.8% R283R (CGT→CGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9670T→C17.8% 114.1 ‑0.6 ‑1.2390R283R (CGT→CGCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (26/23):  ref base (127/141):  total (181/209)
Fisher's exact test strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.66e-02

Only 100 of 430 total aligned reads displayed.

CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1396868‑1397066
                                                                                                                  |                                                                                                    
cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.Tg                                                                                                     <  2:3053645/101‑1 (MQ=255)
        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTcc                                                                                            <  1:2909133/101‑1 (MQ=255)
        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTcc                                                                                            >  2:1233636/1‑101 (MQ=255)
          aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTcccc                                                                                          >  2:1439239/1‑101 (MQ=255)
               ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCcgcg                                                                                     >  2:4995430/1‑101 (MQ=255)
                  cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCgggg                                                                                  <  1:3991391/101‑1 (MQ=255)
                     ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCg                                                                               >  2:1847137/1‑101 (MQ=255)
                      gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGt                                                                              <  2:2958992/101‑1 (MQ=255)
                         ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:3587270/91‑1 (MQ=9)
                         ctcctcAGACGTCTCGCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  2:2635324/101‑1 (MQ=255)
                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGAt                                                                          >  1:1894328/1‑101 (MQ=255)
                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCCCGGGGGCCTgggt                                                                          >  1:4765541/1‑99 (MQ=255)
                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         >  1:1945509/9‑101 (MQ=2)
                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         >  1:1501469/9‑101 (MQ=2)
                           cctcgcaCGTCGGCCCCCCCGCCGGGGCGTGGATGGAAGCAA..TG.CG.GCGCG.GG.CCCA..T.GGCT.GC.CGG.ACC..CGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:4801567/95‑1 (MQ=0)
                           cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  1:936471/101‑1 (MQ=255)
                           cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCCCCCCCCGCGGGGGCGTGGATc                                                                         <  1:2874552/101‑1 (MQ=255)
                            ngcncnngTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  1:2165525/94‑1 (MQ=2)
                            cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:719953/95‑1 (MQ=2)
                            caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         <  2:951001/94‑1 (MQ=9)
                             ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GG.CTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  1:2327536/6‑101 (MQ=2)
                             gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       >  2:3462617/7‑101 (MQ=2)
                             gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       >  2:3991955/7‑101 (MQ=2)
                              ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  2:4591282/96‑1 (MQ=2)
                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                                      >  1:635910/1‑101 (MQ=255)
                              acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       >  1:1521890/6‑101 (MQ=9)
                               gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:4175570/4‑101 (MQ=2)
                               aggccccGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:2260256/95‑1 (MQ=255)
                               aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                                     >  1:429884/1‑101 (MQ=255)
                                tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GG.CTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:180527/99‑1 (MQ=2)
                                 ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  2:230965/99‑1 (MQ=9)
                                 ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    >  2:312723/3‑101 (MQ=9)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                                  <  1:2707310/100‑3 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                                  <  1:2001456/100‑3 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A...A.CT.GACC...C.GGCA..ACGGCC.TA.TGCGCTGGACG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                                  >  2:4049441/2‑99 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    >  1:412049/1‑101 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                                  >  1:1057255/1‑99 (MQ=9)
                                   gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.A..CC.CT.GCGCC.GC.AGCG..AGTGCG.TCTGGA.ACA..GG.AT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                                  <  2:4434227/101‑3 (MQ=2)
                                    tCGCTCCACCCGCGGGGTCGTGGGCCGAAAC.A..ACGCG.GCGCG.GG.CCTA..T.GGCTGTC.GCC.CCC..CC.CC.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAActgc                                                                 <  2:710065/101‑4 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                  <  2:1704308/101‑2 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                  >  1:1221739/1‑100 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATTGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGATCCCCCCGCGGGGGGGTGGTTCGAAACATAAGc                                                              >  1:4064415/1‑101 (MQ=37)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.AT.GG.CTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                               <  1:3386612/101‑6 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                              >  1:3242347/1‑101 (MQ=255)
                                      ccTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                                 <  2:3235949/100‑3 (MQ=2)
                                       ccccccccGCGGGGGCGTGGGTCCAAAC.ATTGC.TT.TGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                              <  2:2766090/99‑7 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                                <  1:3411648/101‑1 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCATCt                                                             >  2:1133706/1‑101 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                               <  1:2944103/101‑2 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                             <  2:4739187/101‑8 (MQ=9)
                                        cccccccGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                               <  1:223403/100‑2 (MQ=2)
                                        cccccccGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                             <  1:4517929/100‑8 (MQ=9)
                                         ccccccGCGGGGGCGTGGATCGAAAC.AT.GG.CTATGGCG.GC.AGTG..TCAGGT.T..TTC.CAG..CG.GC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAccccccacc                                                            >  2:1299302/1‑93 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                           <  1:2642444/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                            >  1:2809388/1‑93 (MQ=9)
                                          cccccGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                          <  1:3876262/101‑1 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                           <  2:4921676/101‑10 (MQ=9)
                                          cccccGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                           <  1:1301550/101‑10 (MQ=9)
                                          cccccGCGGGGGCGTGGATCGAAAC....GC.TG.GCGCGCGC.CGCG..CCGGATCTT.TGT.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                           <  2:1579696/101‑10 (MQ=9)
                                           ccccGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CC.ATGAGTTCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                            <  2:4299223/101‑5 (MQ=0)
                                            cccGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                                        <  2:2885350/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                          <  1:2469531/101‑7 (MQ=0)
                                             ccGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                                       >  2:1004960/1‑101 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAAC.T..GC.GT.GCGCG.CCATGAAT.TCATCG.AG.CGC.CAT..CG.AGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                         <  2:3670359/101‑8 (MQ=0)
                                              cgcgGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                                      <  1:210884/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.GG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGTCGTTgg                                                    >  1:3792349/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAAC.A..TC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.CG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                                                   >  1:877486/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                                   >  1:4378169/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                                   <  2:4675463/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                                <  2:1098654/101‑1 (MQ=255)
                                                     gCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                               >  2:779099/1‑101 (MQ=255)
                                                      gggggATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                              <  2:1504348/98‑1 (MQ=255)
                                                       gTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                             <  1:3623409/101‑1 (MQ=255)
                                                       gTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGGTGGGCAGCCg                                             >  2:4335797/1‑101 (MQ=255)
                                                        tGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                            <  2:3311936/101‑1 (MQ=255)
                                                         ggATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                           <  2:3755266/101‑1 (MQ=255)
                                                         ggATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                           >  1:375204/1‑101 (MQ=255)
                                                           aTCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                         >  2:2654078/1‑101 (MQ=255)
                                                              gAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                      >  1:309201/1‑101 (MQ=255)
                                                               aaaC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                                     >  1:4963719/1‑101 (MQ=255)
                                                               aaaC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                                     >  1:1359769/1‑101 (MQ=255)
                                                                aaC.A..GC.CT.GGGCG.GC.CTCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                                    <  1:1468078/101‑1 (MQ=255)
                                                                aaC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                                    <  1:1694266/101‑1 (MQ=255)
                                                                 aC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                                   >  2:1913806/1‑101 (MQ=255)
                                                                  c.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                                  >  1:4876284/1‑101 (MQ=255)
                                                                    a..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt                                 >  1:2400336/1‑101 (MQ=255)
                                                                       gC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                                <  2:2825696/101‑1 (MQ=255)
                                                                        c.cT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTcc                               >  2:2398575/1‑100 (MQ=255)
                                                                             gcgcG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                            >  2:1358561/1‑101 (MQ=255)
                                                                              cgcg.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc                           >  1:3527274/1‑101 (MQ=255)
                                                                                       gCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg                    <  1:4434090/101‑1 (MQ=255)
                                                                                         a..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg                  >  2:56684/1‑101 (MQ=255)
                                                                                           ctCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg                  <  2:2185965/100‑1 (MQ=255)
                                                                                             cGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg                >  1:4604600/1‑101 (MQ=255)
                                                                                                 t.tC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg            >  1:3681461/1‑101 (MQ=255)
                                                                                                   tC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTgg           <  2:1969976/101‑1 (MQ=255)
                                                                                                       gC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:4256292/101‑1 (MQ=255)
                                                                                                       cgtcTG..CG.TG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:3176767/98‑1 (MQ=255)
                                                                                                          cTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa      <  1:4209733/101‑1 (MQ=255)
                                                                                                               cG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  1:5047332/101‑1 (MQ=255)
                                                                                                                  |                                                                                                    
CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.A..GC.CT.GCGCG.GC.CGCA..TCGGCT.TC.CGC.CTG..CG.TG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1396868‑1397066

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG