BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,066	T→G	8.0%	T316T (ACT→ACG)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,066	0	T→G	8.0%	55.7 ‑3.6 ‑1.4	289	T316T (ACT→ACG)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (5/18): ref base (123/143): total (128/161)
Fisher's exact test strand distribution p-value = 2.80e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.66e-02

Only 100 of 307 total aligned reads displayed.


GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166


  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    >  1:1616310/1‑101 (MQ=255)


  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    >  2:2425870/1‑101 (MQ=255)


  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    <  2:2526101/101‑1 (MQ=255)


    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                                                                    >  2:3784331/1‑101 (MQ=2)


     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                                                                                   >  2:1747409/1‑100 (MQ=2)


      gCTCCCCCCGCGGGGGCGTGGATCGAGACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                                                                  <  1:1866279/101‑3 (MQ=2)


      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                                                                  <  2:4764724/101‑3 (MQ=2)


      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                                                                  >  2:3113324/1‑99 (MQ=2)


      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                                                                                >  1:1044910/1‑101 (MQ=255)


       cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                                                                               >  2:369588/1‑101 (MQ=255)


        tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGGcgtag                                                                                                >  1:665181/1‑97 (MQ=2)


         ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                                                                               >  1:241835/1‑96 (MQ=2)


         ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                                                                               <  1:1886523/101‑6 (MQ=2)


         ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                                                             <  2:4145247/101‑1 (MQ=255)


         ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                                                             <  1:304144/101‑1 (MQ=255)


          cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                                                                                            <  2:1936862/101‑1 (MQ=255)


             ccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                                                                                         <  1:2549282/101‑1 (MQ=255)


              cgcgGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctcg                                                                                          <  1:1747539/101‑11 (MQ=0)


              cgcgGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACGGcgtagatctcg                                                                                          >  2:1146080/1‑91 (MQ=0)


                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     >  2:2786133/1‑101 (MQ=255)


                  ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATggg                                                                                    >  1:4969445/1‑101 (MQ=255)


                  ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                                                                                    >  1:3977350/1‑101 (MQ=255)


                    ggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGCgc                                                                                  <  2:4485193/101‑1 (MQ=255)


                     gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcg                                                                                 >  1:3714759/1‑101 (MQ=255)


                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               <  2:2233299/101‑1 (MQ=255)


                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCCGTCTTGAGTGATGGGGggcg                                                                               >  1:46075/1‑101 (MQ=255)


                        tGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                                                                              >  2:1156476/1‑101 (MQ=255)


                        tGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                                                                              >  2:3244707/1‑101 (MQ=255)


                        tGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                                                                              >  1:4349962/1‑101 (MQ=255)


                         ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcggg                                                                             >  1:3222245/1‑99 (MQ=255)


                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGCGAGc                                                                            >  1:387263/1‑101 (MQ=255)


                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:4839423/101‑1 (MQ=255)


                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:703746/101‑1 (MQ=255)


                            tCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                                                                          <  1:958945/101‑1 (MQ=255)


                             cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                                                                         <  1:779201/101‑1 (MQ=255)


                             cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                                                                         <  2:3517485/101‑1 (MQ=255)


                             cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                                                                         <  1:4476276/101‑1 (MQ=255)


                              gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                                                        >  1:3866263/1‑101 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATTTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       <  1:4302533/101‑1 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       <  2:2360167/101‑1 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       >  1:3728131/1‑101 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       <  2:4414596/101‑1 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       >  2:4453112/1‑101 (MQ=255)


                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAA.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       <  1:4124699/101‑1 (MQ=255)


                                aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                                                                      >  1:4697894/1‑101 (MQ=255)


                                       gCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCCCATGTCGTCGCt                                                               >  2:1223527/1‑101 (MQ=255)


                                        cTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGACCACATGCCGTCGccc                                                              >  1:2759570/1‑99 (MQ=255)


                                          cg.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                                                            <  1:2364416/101‑1 (MQ=255)


                                           g.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc                                                           >  2:3895970/1‑101 (MQ=255)


                                             tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGGACATGTCGTCCCTtcccc                                                          >  2:3389688/1‑101 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         <  1:1516515/101‑1 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         >  1:2949055/1‑101 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         >  2:1041592/1‑101 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         >  1:1945537/1‑101 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         >  1:2107889/1‑101 (MQ=255)


                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         <  2:1697549/101‑1 (MQ=255)


                                               gtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg                                                        >  1:1703012/1‑101 (MQ=255)


                                                ttGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc                                                       <  2:4374883/101‑1 (MQ=255)


                                                 tGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg                                                      >  2:1418991/1‑101 (MQ=255)


                                                   gTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCggg                                                    <  1:1005070/101‑1 (MQ=255)


                                                      aGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGc                                                 <  2:2718569/101‑1 (MQ=255)


                                                      aGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCCAGCACATGTCGTCGCTCCCCCCCCGGGGGc                                                 >  2:4647760/1‑101 (MQ=255)


                                                       gCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCg                                                >  2:3401754/1‑101 (MQ=255)


                                                        ccGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCgg                                               >  1:2287991/1‑100 (MQ=255)


                                                         cGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTg                                              <  2:2327713/101‑1 (MQ=255)


                                                          ggAC.GATGTCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:2654263/101‑1 (MQ=255)


                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCCCATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:2834230/101‑1 (MQ=255)


                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  1:3472089/101‑1 (MQ=255)


                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGa                                            >  1:4409997/1‑101 (MQ=255)


                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:1824056/1‑101 (MQ=255)


                                                            aC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGAt                                           >  1:1801572/1‑101 (MQ=255)


                                                                aTGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:264064/1‑101 (MQ=255)


                                                                    tcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  2:4056193/100‑1 (MQ=255)


                                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGt                                   <  2:4265579/101‑1 (MQ=255)


                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  >  1:2147758/1‑101 (MQ=255)


                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTGGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  <  2:3020930/101‑1 (MQ=255)


                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                >  1:2599265/1‑101 (MQ=255)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                               <  1:1399390/101‑1 (MQ=255)


                                                                           tCCCCCCGCGGGGGCGTGGATCTAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                             <  2:20539/101‑1 (MQ=255)


                                                                            ccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTc                            >  1:4767056/1‑101 (MQ=255)


                                                                              tccgatctgggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgc                          <  1:3768535/93‑1 (MQ=255)


                                                                                    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                    <  1:3389951/101‑1 (MQ=255)


                                                                                    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                    <  2:3662349/101‑1 (MQ=255)


                                                                                     ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                   >  1:2687601/1‑101 (MQ=255)


                                                                                     ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                   >  1:3084085/1‑101 (MQ=255)


                                                                                     ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                   <  1:4839965/101‑1 (MQ=255)


                                                                                      gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcgcagatcgga                  >  2:3768250/1‑93 (MQ=255)


                                                                                        gCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTc                <  2:2107741/101‑1 (MQ=255)


                                                                                          gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGGAAAGTCCCGCCCCCGCCCCATGTcct              >  2:4565893/1‑99 (MQ=37)


                                                                                           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc             <  2:2287827/101‑1 (MQ=255)


                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGTGCCACATGTCTTCg            >  1:5018560/1‑101 (MQ=255)


                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            >  2:5033302/1‑101 (MQ=255)


                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            >  1:3304151/1‑101 (MQ=255)


                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         <  1:1358687/101‑1 (MQ=255)


                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         >  2:3147732/1‑101 (MQ=255)


                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         <  1:1233753/101‑1 (MQ=255)


                                                                                                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgc     <  1:13900/101‑1 (MQ=255)


                                                                                                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgc     <  1:1150182/101‑1 (MQ=255)


                                                                                                     cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgcgc   <  2:2068872/101‑1 (MQ=255)


                                                                                                      tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGcc  <  1:859195/101‑1 (MQ=255)


GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG