Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,343 C→G 1.8% P409A (CCT→GCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3430C→G 1.8% 3.0 ‑1.6 ‑0.0273P409A (CCT→GCT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (129/139):  total (133/140)
Fisher's exact test strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.12e-01

Only 100 of 284 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCC  >  NC_014034/1397243‑1397443
                                                                                                     |                                                                                                    
cccGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGc                                                                                                      <  2:2270952/101‑1 (MQ=255)
   gcgGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                                                   <  1:2914736/101‑1 (MQ=255)
    cgGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGTTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                                                  >  2:1987555/1‑101 (MQ=255)
     gggggCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                                 >  2:3761239/1‑101 (MQ=255)
     gggggCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                                 >  1:4978078/1‑101 (MQ=255)
           gTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                                           <  2:4977748/101‑1 (MQ=255)
           gTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATAGAAACGGGTTT.ACAGCCTTCGCGcccc                                                                                           >  1:1283430/1‑101 (MQ=255)
            tGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCACCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                                          >  1:3719317/1‑101 (MQ=255)
             ggATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                                         >  1:211234/1‑101 (MQ=255)
              gATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                                        >  1:2826346/1‑101 (MQ=255)
               aTCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGc                                                                                       <  2:4045644/101‑1 (MQ=255)
                tCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                                                      <  2:307173/101‑1 (MQ=255)
                 cGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                                                     >  1:686959/1‑101 (MQ=255)
                 cGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACCCCa                                                                                     >  2:4139615/1‑101 (MQ=255)
                  gAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAcagatc                                                                                    >  2:3335861/1‑96 (MQ=255)
                  gAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                                                    >  1:2508010/1‑101 (MQ=255)
                  gAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                                                    <  1:840916/101‑1 (MQ=255)
                  gAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                                                    >  1:4041647/1‑101 (MQ=255)
                   aaaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                                   >  2:3953420/1‑101 (MQ=255)
                   aaaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                                   <  1:1857687/101‑1 (MQ=255)
                    accacCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                                  <  1:1180911/99‑1 (MQ=255)
                    aaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                                  <  2:2593318/101‑1 (MQ=255)
                    aaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAAGGATTT.ACAGCGTTCGCGGCCACCGCCAgcg                                                                                  >  1:3791530/1‑99 (MQ=37)
                      cacCGCGCACGCGCGAACGGCGTCAGCCCCGCGACCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                                                <  2:345451/101‑1 (MQ=255)
                       acCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgga                                                                               <  1:2156765/101‑1 (MQ=255)
                        ccGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                                              <  1:4657480/101‑1 (MQ=255)
                        ccGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                                              <  2:4385011/101‑1 (MQ=255)
                          gcgcACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                                            <  2:981185/101‑1 (MQ=255)
                              aCGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                                                                        >  1:2757240/1‑101 (MQ=255)
                                   cgAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                                                                   >  2:2853124/1‑101 (MQ=255)
                                    gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                  >  2:4855946/1‑101 (MQ=255)
                                    gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                  >  2:3519107/1‑101 (MQ=255)
                                    gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                  >  2:2800466/1‑101 (MQ=255)
                                     aaCGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                                                 <  1:1149461/101‑1 (MQ=255)
                                         gCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggg                                                             >  1:3310965/1‑101 (MQ=255)
                                          cGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                                            >  2:3144358/1‑101 (MQ=255)
                                          cGTCAGCCCCGCGAACGTCGCTCACCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                                            <  1:1044832/101‑1 (MQ=255)
                                              aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt                                                        >  1:164575/1‑101 (MQ=255)
                                              aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt                                                        <  1:2640446/101‑1 (MQ=255)
                                              aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt                                                        <  1:3203879/101‑1 (MQ=255)
                                               gCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg                                                       <  2:3922773/101‑1 (MQ=255)
                                                  ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:4771112/101‑1 (MQ=255)
                                                   cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:494659/101‑1 (MQ=255)
                                                   cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  1:2385727/1‑101 (MQ=255)
                                                       aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  2:2310580/101‑1 (MQ=255)
                                                        aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              >  1:3082502/1‑101 (MQ=255)
                                                        aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  2:2174435/101‑1 (MQ=255)
                                                         cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             >  2:4318831/1‑101 (MQ=255)
                                                           tCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                           >  1:4233684/1‑101 (MQ=255)
                                                            cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTCtt                                          >  1:945175/1‑101 (MQ=255)
                                                            cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                                          >  2:2653787/1‑101 (MQ=255)
                                                            cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACTCtt                                          >  1:4327613/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                                         >  1:4569116/1‑101 (MQ=255)
                                                              cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                                        >  2:3804471/1‑101 (MQ=255)
                                                               tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa                                       >  2:1068571/1‑101 (MQ=255)
                                                               cccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa                                       <  1:3424043/100‑1 (MQ=255)
                                                               cccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa                                       <  2:1732669/100‑1 (MQ=255)
                                                                ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt                                      <  1:1013614/101‑1 (MQ=255)
                                                                ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt                                      <  1:3031824/101‑1 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc                                   >  1:502182/1‑91 (MQ=2)
                                                                    ccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGat                                  <  1:1513386/101‑1 (MQ=255)
                                                                     cgcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGata                                 >  2:4543770/1‑101 (MQ=255)
                                                                      gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat                                <  1:3144596/101‑1 (MQ=255)
                                                                      gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat                                >  2:2573758/1‑101 (MQ=255)
                                                                        gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                              <  2:755059/101‑1 (MQ=255)
                                                                        gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                              <  1:3830149/101‑1 (MQ=255)
                                                                        gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                              >  1:449678/1‑101 (MQ=255)
                                                                        gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCTTCAATGGATATgg                              <  1:3123610/101‑1 (MQ=255)
                                                                          gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa                            >  1:2542477/1‑101 (MQ=255)
                                                                          gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa                            <  1:3674554/101‑1 (MQ=255)
                                                                          gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa                            >  1:411064/1‑101 (MQ=255)
                                                                          gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa                            <  2:518448/101‑1 (MQ=255)
                                                                           aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa                           >  2:1425249/1‑101 (MQ=255)
                                                                           aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa                           <  2:4344261/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaaaa                         <  2:4969193/101‑1 (MQ=255)
                                                                              gTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAc                        >  2:2970952/1‑101 (MQ=255)
                                                                               tGGATCGAAACGGCTTT.ACAGGCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAcc                       >  1:2869969/1‑101 (MQ=255)
                                                                                ggATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGTAGCGAAACTCTTCAATGGATATGGCAAAAccc                      >  2:667197/1‑100 (MQ=255)
                                                                                    cGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcg                  >  1:3150935/1‑101 (MQ=255)
                                                                                     gAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgc                 <  2:1146855/101‑1 (MQ=255)
                                                                                     gAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgc                 >  2:3663093/1‑101 (MQ=255)
                                                                                     gAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgc                 >  1:2069818/1‑101 (MQ=255)
                                                                                       aaCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGa               >  2:1272651/1‑101 (MQ=255)
                                                                                        aCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGAc              >  2:1495638/1‑101 (MQ=255)
                                                                                          ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt            >  2:688337/1‑101 (MQ=255)
                                                                                          ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt            >  2:1410884/1‑101 (MQ=255)
                                                                                           gCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATg           >  2:3209978/1‑101 (MQ=255)
                                                                                           gCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATg           <  2:2194027/101‑1 (MQ=255)
                                                                                            cTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGt          <  2:408539/101‑1 (MQ=255)
                                                                                            cTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGt          >  2:2121741/1‑101 (MQ=255)
                                                                                             ttt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTc         <  1:1987699/101‑1 (MQ=255)
                                                                                             ttt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTc         >  1:1544052/1‑101 (MQ=255)
                                                                                             ttt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTc         <  2:1162635/101‑1 (MQ=255)
                                                                                              tt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCg        <  2:468561/101‑1 (MQ=255)
                                                                                              tt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCg        >  2:3721875/1‑101 (MQ=255)
                                                                                              tt.ACAGCCTTCCCGCCCCACGCCAGACGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCg        <  2:4765218/101‑1 (MQ=255)
                                                                                              ct.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCg        <  2:1609508/100‑1 (MQ=255)
                                                                                               t.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGc       >  1:3851072/1‑101 (MQ=255)
                                                                                                 aCAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCt      <  2:2409037/101‑1 (MQ=255)
                                                                                                    gCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTccc   <  1:1223644/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCC  >  NC_014034/1397243‑1397443

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG