Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,669 G→T 1.9% Q517H (CAG→CGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6690G→T 1.9% 2.2 ‑1.0 ‑0.0225Q517H (CAG→CAT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (111/109):  total (112/113)
Fisher's exact test strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCG  >  NC_014034/1397569‑1397769
                                                                                                    |                                                                                                      
cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                        <  2:1600097/101‑1 (MQ=255)
cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                        >  2:2720073/1‑101 (MQ=255)
cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                        >  1:729108/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACaga                                                                                                       <  2:14609/101‑1 (MQ=255)
  ttCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTACagag                                                                                                      >  1:4202203/2‑101 (MQ=255)
     cccccCCGGGGGCGTGGAGCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTc                                                                                                   <  1:637085/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.Cg                                                                                                <  2:3842903/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTACAGGGTTC.Cgg                                                                                               >  1:2199687/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGt                                                                                              <  2:3285781/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTc                                                                                             >  1:212996/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCt                                                                                            >  2:4103809/1‑101 (MQ=255)
            tccgatctGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                           <  2:348115/94‑1 (MQ=255)
            gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                           <  1:1076230/101‑1 (MQ=255)
            gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                           >  1:4663298/1‑101 (MQ=255)
             ggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtg                                                                                          >  2:4828668/1‑101 (MQ=255)
             ggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtg                                                                                          <  1:2564892/101‑1 (MQ=255)
              gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgt                                                                                         <  2:191360/101‑1 (MQ=255)
                gCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttc                                                                                       <  1:2121889/101‑1 (MQ=255)
                 cGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttct                                                                                      <  2:3570487/101‑1 (MQ=255)
                  gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctt                                                                                     >  2:505083/1‑100 (MQ=255)
                  gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctg                                                                                     <  2:4663253/101‑1 (MQ=255)
                  gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctg                                                                                     >  2:2866080/1‑101 (MQ=255)
                    ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCttagatcgga                                                                                   >  1:348168/1‑93 (MQ=255)
                    ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgtt                                                                                   >  1:1613187/1‑101 (MQ=255)
                     gATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTCAGAGTTC.CGGTCTtgttctgttc                                                                                  <  2:4443463/101‑1 (MQ=255)
                     gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgttc                                                                                  >  1:3425769/1‑101 (MQ=255)
                        cGAAACATCCGCGGATCGGCGTGGCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.Cgg                                                                              <  2:498316/101‑1 (MQ=255)
                          aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                            >  1:2721873/1‑101 (MQ=255)
                          aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                            >  2:2584909/1‑101 (MQ=255)
                          aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                            >  1:3943528/1‑101 (MQ=255)
                           aaCACCCGCGGATCGGGGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTaa                                                                           <  1:4470090/101‑1 (MQ=255)
                            aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                          <  2:1166895/101‑1 (MQ=255)
                            aCATCCGCGGATCGGCGCGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                          <  2:4546609/101‑1 (MQ=255)
                             cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                         <  2:4700287/101‑1 (MQ=255)
                             cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                         <  2:1992587/101‑1 (MQ=255)
                             cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                         <  1:3554694/101‑1 (MQ=255)
                              aTCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAg                                                                        >  2:291680/1‑101 (MQ=255)
                              aTCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAg                                                                        <  2:2524106/101‑1 (MQ=255)
                                 cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTc                                                                     <  1:1229291/101‑1 (MQ=255)
                                   cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTNTTC.CGGTAACNNGNNNc                                                                   >  2:1308823/1‑101 (MQ=255)
                                    ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                  >  2:3451127/1‑101 (MQ=255)
                                    ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGCCTTGTTCTGTTC.CGGCAACAGTTCGCt                                                                  >  2:2429101/1‑101 (MQ=255)
                                        cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccc                                                              >  2:587488/1‑101 (MQ=255)
                                         ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccccc                                                             >  2:2016419/1‑101 (MQ=255)
                                         ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccccc                                                             >  2:1729168/1‑101 (MQ=255)
                                           cggtgcccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcg                                                           <  2:1000065/96‑1 (MQ=255)
                                           cGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcg                                                           >  2:4047681/1‑101 (MQ=255)
                                            gTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgc                                                          >  2:4609492/1‑101 (MQ=255)
                                             tGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgcg                                                         >  1:1977587/1‑101 (MQ=255)
                                              gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCgg                                                        >  1:3598992/1‑101 (MQ=255)
                                                  cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGgggc                                                    >  1:1778951/1‑101 (MQ=255)
                                                     cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGNGCGTg                                                 >  1:1000238/1‑101 (MQ=255)
                                                          ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  2:3160805/101‑1 (MQ=255)
                                                           cATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           <  2:2648792/101‑1 (MQ=255)
                                                            aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:1231209/101‑1 (MQ=255)
                                                            aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  1:3378453/1‑101 (MQ=255)
                                                              cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        <  1:2630642/101‑1 (MQ=255)
                                                               ggTCGCTCCCCCCGTGGGGGCGTGGATAGAAACTACATAGTTC.CGCTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                       <  2:2490752/101‑1 (MQ=37)
                                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                       >  2:1749307/1‑101 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTg                                     <  1:559643/101‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                 >  2:3116734/1‑101 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                >  1:4400558/1‑101 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATGGCtatggc                             <  1:4501023/101‑6 (MQ=0)
                                                                         cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggc                             >  1:1338110/1‑96 (MQ=0)
                                                                          ccGCGGGGGCGTGGATCGACTCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcccg                            <  1:2890137/101‑1 (MQ=38)
                                                                          ccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgcg                            <  1:505148/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                           >  1:1421842/1‑101 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                         >  1:2436638/1‑101 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                         >  1:3199903/1‑101 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                         >  2:168613/1‑101 (MQ=255)
                                                                              ngnngNNTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcn                        <  1:1850660/100‑2 (MQ=255)
                                                                              gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                        >  2:4335759/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                        >  1:4106228/1‑101 (MQ=255)
                                                                              aggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGCCGCGCtca                        >  1:22344/2‑101 (MQ=255)
                                                                                gggGGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                      >  2:4276129/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                      <  2:1867191/101‑1 (MQ=255)
                                                                                  gCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                    <  1:3096963/101‑1 (MQ=255)
                                                                                   cGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                   >  2:4192093/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                  >  2:1029228/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                  >  2:2323029/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                  <  1:1739455/101‑1 (MQ=255)
                                                                                     tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                 <  1:223607/101‑1 (MQ=255)
                                                                                     tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                 >  1:404352/1‑101 (MQ=255)
                                                                                     tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                 >  2:664010/1‑101 (MQ=255)
                                                                                      ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                <  2:3684529/101‑1 (MQ=255)
                                                                                      ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                >  2:4769965/1‑101 (MQ=255)
                                                                                       gATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGt               >  1:3624922/1‑101 (MQ=255)
                                                                                        aTCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc              <  2:729019/101‑1 (MQ=255)
                                                                                        aTCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc              <  2:1054127/101‑1 (MQ=255)
                                                                                         tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa             >  1:4764779/1‑101 (MQ=255)
                                                                                         tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa             >  1:806142/1‑101 (MQ=255)
                                                                                           gAAACTACAGAGTTC.CGGTCTTGTTCTGTGC.AGGTAACAGGTCGCACCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg           <  2:4739707/101‑1 (MQ=38)
                                                                                            aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa          >  2:2483789/1‑101 (MQ=255)
                                                                                            aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa          >  1:846906/1‑101 (MQ=255)
                                                                                            aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa          >  1:1169007/1‑101 (MQ=255)
                                                                                             aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt         >  1:3788805/1‑101 (MQ=255)
                                                                                                 aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTAAAGATTCAg     >  2:2173414/1‑101 (MQ=255)
                                                                                                  cAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt    >  2:1398491/1‑101 (MQ=255)
                                                                                                   agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc   <  1:1099327/101‑1 (MQ=255)
                                                                                                    gagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCg  <  2:1613055/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
CGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCG  >  NC_014034/1397569‑1397769

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG