BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,732	T→A	2.4%	intergenic (+36/+1862)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,732	0	T→A	2.4%	6.8 ‑1.7 ‑1.3	204	intergenic (+36/+1862)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (1/4): ref base (109/90): total (110/94)
Fisher's exact test strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.50e-02

Only 100 of 215 total aligned reads displayed.


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAG  >  NC_014034/1397632‑1397806


ggTCGCTCCCCCCGTGGGGGCGTGGATAGAAACTACATAGTTC.CGCTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                            <  2:2490752/101‑1 (MQ=37)


ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                            >  2:1749307/1‑101 (MQ=255)


 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                                                           <  1:1941827/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CAGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                                                       <  2:4157297/101‑1 (MQ=255)


      tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                                      >  2:3116734/1‑101 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                                     <  1:3953712/101‑1 (MQ=255)


          cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggc                                                                  >  1:1338110/1‑96 (MQ=0)


          cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggc                                                                  <  2:4993172/101‑6 (MQ=0)


           ccGCGGGGGCGTGGATCGACTCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcccg                                                                 <  1:2890137/101‑1 (MQ=38)


            cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                                                >  1:1421842/1‑101 (MQ=255)


            cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                                                <  1:2781863/101‑1 (MQ=255)


              cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                              <  2:1948239/101‑1 (MQ=255)


              cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                              >  1:3199903/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                              >  2:168613/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                              >  2:1248618/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                              >  1:2436638/1‑101 (MQ=255)


               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcc                                                             >  1:22345/1‑100 (MQ=255)


               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                             >  2:4335759/1‑101 (MQ=255)


               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                             >  1:4106228/1‑101 (MQ=255)


                ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                                                            <  1:4595764/101‑1 (MQ=255)


                ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                                                            <  2:4967708/101‑1 (MQ=255)


                 ggnCTTGGATCGAAACTNCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTACCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                           <  2:1000128/101‑1 (MQ=255)


                 gggGGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                           >  2:4276129/1‑101 (MQ=255)


                 gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                           <  2:1867191/101‑1 (MQ=255)


                 gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                           <  2:2381454/101‑1 (MQ=255)


                   gCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                                                         <  1:3096963/101‑1 (MQ=255)


                    cGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                                        >  2:4192093/1‑101 (MQ=255)


                     gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                                       <  1:623022/101‑1 (MQ=255)


                     gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                                       <  1:1739455/101‑1 (MQ=255)


                      tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                                      >  2:664010/1‑101 (MQ=255)


                      tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                                      <  2:4662975/101‑1 (MQ=255)


                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                                     >  1:2573573/1‑101 (MQ=255)


                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                                     >  2:4769965/1‑101 (MQ=255)


                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                                     <  2:3684529/101‑1 (MQ=255)


                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                                     >  1:4434546/1‑101 (MQ=255)


                        gATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGt                                                    <  2:2892965/101‑1 (MQ=255)


                         aTCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                                                   <  2:1054127/101‑1 (MQ=255)


                          tCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                                  <  1:2611475/101‑1 (MQ=255)


                           cGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCaa                                                 >  1:1229599/1‑101 (MQ=255)


                            gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                                <  1:587567/101‑1 (MQ=255)


                            gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                                <  2:1656463/101‑1 (MQ=255)


                            gAAACTACAGAGTTC.CGGTCTTGTTCTGTGC.AGGTAACAGGTCGCACCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                                <  2:4739707/101‑1 (MQ=38)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               <  2:1449264/101‑1 (MQ=255)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               >  1:3565070/1‑101 (MQ=255)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               >  2:2726456/1‑101 (MQ=255)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               <  1:836572/101‑1 (MQ=255)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               >  1:846906/1‑101 (MQ=255)


                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                               >  1:1169007/1‑101 (MQ=255)


                              aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                                              >  1:3788805/1‑101 (MQ=255)


                               aCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                             <  1:1693563/101‑1 (MQ=255)


                                 tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa                                           >  2:121796/1‑101 (MQ=255)


                                 tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa                                           >  2:2028464/1‑101 (MQ=255)


                                  aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAg                                          >  2:2593241/1‑101 (MQ=255)


                                  aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTAAAGATTCAg                                          >  2:2173414/1‑101 (MQ=255)


                                    agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                        <  1:1099327/101‑1 (MQ=255)


                                    agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAATc                                        >  2:1363793/1‑101 (MQ=255)


                                     gagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCg                                       <  2:1613055/101‑1 (MQ=255)


                                      agTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                                      >  2:4365197/1‑101 (MQ=255)


                                        ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                                    >  1:3628895/1‑101 (MQ=255)


                                        ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                                    >  1:1580415/1‑101 (MQ=255)


                                        ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                                    <  2:2425975/101‑1 (MQ=255)


                                          c.cGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccc                                  >  2:14924/1‑101 (MQ=255)


                                          c.cGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccc                                  >  1:77836/1‑101 (MQ=255)


                                            nggNCNTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                                 <  1:1308944/100‑1 (MQ=255)


                                            cGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTTCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                                 >  1:4506962/1‑101 (MQ=255)


                                            cGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                                 <  2:877358/101‑1 (MQ=255)


                                             ggTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCTTCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                                <  1:4336076/101‑1 (MQ=255)


                                             ggTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                                >  1:544108/1‑101 (MQ=255)


                                              gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                               <  1:3326575/101‑1 (MQ=255)


                                              gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                               <  1:4047984/101‑1 (MQ=255)


                                              gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                               <  2:282637/101‑1 (MQ=255)


                                              gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                               >  1:2159749/1‑101 (MQ=255)


                                               tCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                              <  2:3943236/101‑1 (MQ=255)


                                                cTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                             >  2:4803647/1‑101 (MQ=255)


                                                cTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                             >  1:4756804/1‑101 (MQ=255)


                                                  tgttctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgg                           >  2:4882663/1‑101 (MQ=255)


                                                  tgttctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgg                           >  2:1312722/1‑101 (MQ=255)


                                                  tgttctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgg                           >  1:4156354/1‑101 (MQ=255)


                                                      ctgttc.CGGTAACAGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                       <  2:212962/101‑1 (MQ=255)


                                                      ctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                       <  2:3356529/101‑1 (MQ=255)


                                                      ctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                       >  2:3638488/1‑101 (MQ=255)


                                                        gttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt                     <  2:3888883/101‑1 (MQ=255)


                                                        gttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt                     >  1:4073987/1‑101 (MQ=255)


                                                        gttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt                     <  1:4000871/101‑1 (MQ=255)


                                                          tc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTgg                   <  1:3455733/101‑1 (MQ=255)


                                                           c.cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGa                  >  2:2535713/1‑101 (MQ=255)


                                                              ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc                >  2:397751/1‑101 (MQ=255)


                                                              ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc                >  1:3350063/1‑101 (MQ=255)


                                                               gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg               >  1:943771/1‑101 (MQ=255)


                                                                tAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa              >  1:1590727/1‑101 (MQ=255)


                                                                tAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa              >  1:4951145/1‑101 (MQ=255)


                                                                  aCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa            >  2:2447186/1‑101 (MQ=255)


                                                                   cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc           >  2:2242571/1‑101 (MQ=255)


                                                                    aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct          <  1:2499663/101‑2 (MQ=255)


                                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc         >  2:4939468/1‑99 (MQ=255)


                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGAGGATCGAAActccgc      >  1:4767455/1‑96 (MQ=255)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggca     <  2:3425505/101‑7 (MQ=255)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggca     <  1:1029338/101‑7 (MQ=255)


                                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcaa    <  2:77826/101‑8 (MQ=255)


                                                                            ccccccGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcaagg  <  2:4400239/101‑10 (MQ=38)


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAG  >  NC_014034/1397632‑1397806

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG