BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,899	A→T	2.3%	intergenic (+203/+1695)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,899	0	A→T	2.3%	3.7 ‑1.0 ‑0.0	218	intergenic (+203/+1695)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (1/4): ref base (101/112): total (102/116)
Fisher's exact test strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 229 total aligned reads displayed.


TGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397800‑1397998


tgtCCAGCGTCACATGCAGCGGCGCCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCGCGACCGCTGTCTCACCaa                                                                                                    >  2:1973961/1‑101 (MQ=255)


 ttCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                                   <  1:611670/100‑1 (MQ=255)


 gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGACCCGCCCGCTGTCTCACCAAc                                                                                                   <  1:2869521/101‑1 (MQ=255)


  tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                  <  2:1662789/101‑1 (MQ=255)


     aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                               <  1:4420514/101‑1 (MQ=255)


       cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                                             >  1:4976946/1‑101 (MQ=255)


       cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                                             <  2:4694791/101‑1 (MQ=255)


        gTCAGATGCAGCGGCGTCGCATCGCCTGGTCGCACACCGCGCGGGGGAGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTGTCATCAACAGTCGCt                                                                                            <  2:2087660/101‑1 (MQ=255)


        gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                            >  2:4132611/1‑101 (MQ=255)


           acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                         >  2:3906516/1‑101 (MQ=255)


           acaTGCAGCGGCGGCGCATCGCCATGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                         <  1:3333408/101‑1 (MQ=255)


            caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                                                        >  2:4309560/1‑101 (MQ=255)


              tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                                      <  1:2011444/101‑1 (MQ=255)


               gCAGCGGCGTCGGATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCTCTCCCCCcg                                                                                     >  1:2745707/1‑101 (MQ=255)


                cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                    <  1:19229/101‑1 (MQ=255)


                 aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                                                   >  1:377590/1‑101 (MQ=255)


                    ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                                <  1:4876987/101‑1 (MQ=255)


                      cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                                              >  2:103111/1‑101 (MQ=255)


                       gTCGCATCGCCATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCg                                                                             <  1:3449729/101‑1 (MQ=255)


                         cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCggg                                                                           >  2:2884200/1‑99 (MQ=255)


                         cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  1:2266806/1‑101 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggnnnnnn                                                                          >  1:1580214/1‑95 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  2:4573997/1‑101 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGCCGTgg                                                                          >  1:561351/1‑101 (MQ=255)


                           cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGggg                                                                         >  2:4399405/1‑100 (MQ=255)


                            aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGggcg                                                                        >  2:3551976/1‑99 (MQ=255)


                             tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:4741655/1‑101 (MQ=255)


                             tCGCCATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:3670259/101‑1 (MQ=255)


                              cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:1708149/101‑1 (MQ=255)


                                 cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:3935742/1‑101 (MQ=255)


                                  aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCCGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  >  1:547523/1‑101 (MQ=255)


                                  aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  2:2032406/101‑1 (MQ=255)


                                    gtccccccccccGCGGGGGCGTGGATCGAACCCCGGCACCTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                                <  1:963978/95‑1 (MQ=38)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                            <  1:1022328/101‑1 (MQ=255)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                            >  1:2203826/1‑101 (MQ=255)


                                         tCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                                           >  1:3273607/1‑101 (MQ=255)


                                         tCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTGTATgg                                                           >  2:2198977/1‑101 (MQ=255)


                                         cccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                                           <  1:3109770/100‑1 (MQ=255)


                                          ccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                          >  2:1087095/1‑101 (MQ=255)


                                          ccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                          >  2:3357801/1‑101 (MQ=255)


                                           cccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                                                         >  2:2345322/1‑101 (MQ=255)


                                            ccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                        <  1:1043226/101‑1 (MQ=255)


                                             cccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                                                       <  1:928407/101‑1 (MQ=255)


                                                gcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                                    <  1:4236849/101‑1 (MQ=255)


                                                gcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                                    <  2:3802599/101‑1 (MQ=255)


                                                 cgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                   >  1:3005354/1‑101 (MQ=255)


                                                 cgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                   <  1:3264513/101‑1 (MQ=255)


                                                  gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                                  >  1:1738625/1‑101 (MQ=255)


                                                   ggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctcggg                                                 >  2:2370191/1‑101 (MQ=255)


                                                   ggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                                                 <  2:5017027/101‑1 (MQ=255)


                                                        gTGGGTCGAAACCCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                            <  1:1087208/101‑1 (MQ=255)


                                                        gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                            <  2:3342581/101‑1 (MQ=255)


                                                        gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggggggctagggaggct                                            >  2:3410166/1‑101 (MQ=255)


                                                         tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                           <  1:1872360/101‑1 (MQ=255)


                                                         tGGATCAAAACCCGGCGCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                           <  2:2557915/101‑1 (MQ=255)


                                                          ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                          >  2:4932976/1‑101 (MQ=255)


                                                           gATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                                         <  1:3001536/101‑1 (MQ=255)


                                                             tCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                       <  2:849661/101‑1 (MQ=255)


                                                             tCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                       <  1:4059972/101‑1 (MQ=255)


                                                              cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGTGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                      >  1:297448/1‑101 (MQ=255)


                                                              cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                      <  1:4604045/101‑1 (MQ=255)


                                                               gAACCCCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                                     <  1:2140899/101‑1 (MQ=255)


                                                               gAACCCCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGCCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTAGGGGAGGCTAGGGAGGCTACGCCcc                                     <  2:1500079/101‑1 (MQ=38)


                                                                aaaCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                                    >  2:4066837/1‑101 (MQ=255)


                                                                 aaCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                                   <  2:1571368/101‑1 (MQ=255)


                                                                  aCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGGGGCTAGGGAGGCTACGCACCTTc                                  >  2:2063379/1‑101 (MQ=255)


                                                                    ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                                >  2:134563/1‑101 (MQ=255)


                                                                    ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                                >  2:4836633/1‑101 (MQ=255)


                                                                     cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:2876786/101‑1 (MQ=255)


                                                                     cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:3947959/101‑1 (MQ=255)


                                                                     cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:1557547/101‑1 (MQ=255)


                                                                     cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:4948981/101‑1 (MQ=255)


                                                                      ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                              <  2:3081793/101‑1 (MQ=255)


                                                                      ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                              <  2:891683/101‑1 (MQ=255)


                                                                        cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                            <  1:2385853/101‑1 (MQ=255)


                                                                        cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                            <  2:954841/101‑1 (MQ=255)


                                                                         aCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTc                           >  2:477596/1‑101 (MQ=255)


                                                                            tCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCt                        >  2:4283044/1‑101 (MQ=255)


                                                                              aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGAACCTTCAAAAGTCGATcc                      >  2:538843/1‑101 (MQ=255)


                                                                              aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcc                      <  1:4261346/101‑1 (MQ=255)


                                                                               gCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcac                     >  2:656702/1‑99 (MQ=255)


                                                                                ccccGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccc                    >  1:1775632/1‑101 (MQ=255)


                                                                                  ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc                  <  1:4277830/101‑1 (MQ=255)


                                                                                  ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc                  <  2:3517891/101‑1 (MQ=255)


                                                                                   cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                 <  1:4309877/101‑1 (MQ=255)


                                                                                   cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACTCTATGGGAGGCTGGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                 <  2:2233412/101‑1 (MQ=255)


                                                                                        gcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCgggg            <  1:3867104/101‑1 (MQ=255)


                                                                                          tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCCCGGGGGc          >  1:3539457/1‑101 (MQ=255)


                                                                                           gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg         >  2:1887686/1‑101 (MQ=255)


                                                                                           gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg         >  1:3365092/1‑101 (MQ=255)


                                                                                           gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg         >  2:4430439/1‑101 (MQ=255)


                                                                                             ctcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTg       >  1:4053489/1‑101 (MQ=255)


                                                                                             ctcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTg       >  2:1766305/1‑101 (MQ=255)


                                                                                               cACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTTGGGAGGCTACGCACCTTCAAAAGAGGCTCCGCCCGCGGGGGGGTGGa     >  2:4054196/1‑101 (MQ=37)


                                                                                               cACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGa     >  2:4939704/1‑101 (MQ=255)


                                                                                                cccAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:932140/100‑1 (MQ=255)


                                                                                                aCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:3870965/101‑1 (MQ=255)


                                                                                                  cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATTGGATGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:3410429/101‑1 (MQ=255)


                                                                                                  cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:4171767/101‑1 (MQ=255)


                                                                                                  cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:3927550/101‑1 (MQ=255)


TGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397800‑1397998

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG