BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,900	A→G	2.3%	intergenic (+204/+1694)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,900	0	A→G	2.3%	2.5 ‑1.0 ‑0.2	218	intergenic (+204/+1694)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (1/4): ref base (101/112): total (102/116)
Fisher's exact test strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.91e-01

Only 100 of 228 total aligned reads displayed.


TGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397800‑1397998


tgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCaa                                                                                                    >  1:4949081/1‑101 (MQ=255)


tgtCCAGCGTCACATGCAGCGGCGCCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCGCGACCGCTGTCTCACCaa                                                                                                    >  2:1973961/1‑101 (MQ=255)


 gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGACCCGCCCGCTGTCTCACCAAc                                                                                                   <  1:2869521/101‑1 (MQ=255)


   ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                                 <  2:166677/101‑1 (MQ=255)


    cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                                <  2:1060006/101‑1 (MQ=255)


     aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                               <  1:4420514/101‑1 (MQ=255)


       cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                                             <  2:4694791/101‑1 (MQ=255)


        gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                            >  2:4132611/1‑101 (MQ=255)


        gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                            <  1:1829035/101‑1 (MQ=255)


           acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                         >  1:3262795/1‑101 (MQ=255)


           acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                         >  2:3906516/1‑101 (MQ=255)


           acaTGCAGCGGCGGCGCATCGCCATGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                         <  1:3333408/101‑1 (MQ=255)


            caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                                                        >  2:4309560/1‑101 (MQ=255)


              tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                                      <  1:2011444/101‑1 (MQ=255)


               gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTTTCTCACCAACAGTCGCTCCCCCcg                                                                                     <  2:3964829/101‑1 (MQ=255)


               gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                     <  1:2500196/101‑1 (MQ=255)


               gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                     <  2:3693328/101‑1 (MQ=255)


                cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                    <  1:19229/101‑1 (MQ=255)


                    ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                                <  1:4876987/101‑1 (MQ=255)


                    ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCCCgggg                                                                                >  1:4733042/1‑101 (MQ=255)


                       gTCGCATCGCCATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCg                                                                             <  1:3449729/101‑1 (MQ=255)


                         cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:1895833/1‑101 (MQ=255)


                         cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  1:2266806/1‑101 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggnnnnnn                                                                          >  1:1580214/1‑95 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  2:4573997/1‑101 (MQ=255)


                          gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGCCGTgg                                                                          >  1:561351/1‑101 (MQ=255)


                           ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:157760/92‑1 (MQ=0)


                           cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  2:4474435/101‑1 (MQ=255)


                           cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:69878/101‑1 (MQ=255)


                           cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGggg                                                                         >  2:4399405/1‑100 (MQ=255)


                            aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGggcg                                                                        >  2:3551976/1‑99 (MQ=255)


                              cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:1708149/101‑1 (MQ=255)


                                  aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCCGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  >  1:547523/1‑101 (MQ=255)


                                  aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  2:2032406/101‑1 (MQ=255)


                                    gtccccccccccGCGGGGGCGTGGATCGAACC.CCGGCACCTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                                <  1:963978/95‑1 (MQ=38)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                            >  1:2203826/1‑101 (MQ=255)


                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                            <  1:1022328/101‑1 (MQ=255)


                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                                           >  1:3273607/1‑101 (MQ=255)


                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTGTATgg                                                           >  2:2198977/1‑101 (MQ=255)


                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCAGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCCTATgg                                                           >  1:4275481/1‑101 (MQ=255)


                                         cccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                                           <  1:3109770/100‑1 (MQ=255)


                                          ccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                          >  2:3357801/1‑101 (MQ=255)


                                          ccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                          <  1:937956/101‑1 (MQ=255)


                                            ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                        >  1:4042111/1‑101 (MQ=255)


                                            ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                        <  1:1043226/101‑1 (MQ=255)


                                              ccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                                                      <  1:499641/101‑1 (MQ=255)


                                                gcgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                                    <  2:3802599/101‑1 (MQ=255)


                                                 cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                   >  1:4756740/1‑101 (MQ=255)


                                                 cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                   >  1:3005354/1‑101 (MQ=255)


                                                  gggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                                  >  1:1738625/1‑101 (MQ=255)


                                                   ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctcggg                                                 >  2:2370191/1‑101 (MQ=255)


                                                   ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                                                 <  2:2349878/101‑1 (MQ=255)


                                                        gTGGGTCGAAAC.CCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                            <  1:1087208/101‑1 (MQ=255)


                                                        gTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                            <  2:3342581/101‑1 (MQ=255)


                                                         tGGATCAAAAC.CCGGCGCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                           <  2:2557915/101‑1 (MQ=255)


                                                          ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                          >  2:4932976/1‑101 (MQ=255)


                                                          ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                          <  2:5029748/101‑1 (MQ=255)


                                                           gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                                         <  1:3001536/101‑1 (MQ=255)


                                                            aTCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc                                        <  2:2070125/101‑1 (MQ=255)


                                                             tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                       <  1:4059972/101‑1 (MQ=255)


                                                             tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                       <  2:849661/101‑1 (MQ=255)


                                                              cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGTGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                      >  1:297448/1‑101 (MQ=255)


                                                               gAACC.CCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                                     <  1:2140899/101‑1 (MQ=255)


                                                               gAACC.CCGGCCCCTCAGCCCCGCCCGCTGTCTCACCAACAGCCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTAGGGGAGGCTAGGGAGGCTACGCCcc                                     <  2:1500079/101‑1 (MQ=38)


                                                                aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                                    >  2:4066837/1‑101 (MQ=255)


                                                                     ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                                >  2:134563/1‑101 (MQ=255)


                                                                     ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                                >  2:4836633/1‑101 (MQ=255)


                                                                      cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:2876786/101‑1 (MQ=255)


                                                                      cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:1557547/101‑1 (MQ=255)


                                                                      cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                               <  1:3947959/101‑1 (MQ=255)


                                                                       ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                              <  2:3081793/101‑1 (MQ=255)


                                                                       ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                              <  2:891683/101‑1 (MQ=255)


                                                                         cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                            <  1:2385853/101‑1 (MQ=255)


                                                                         cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                            <  2:954841/101‑1 (MQ=255)


                                                                          aCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTc                           >  2:477596/1‑101 (MQ=255)


                                                                           ccTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCg                          >  2:29875/1‑101 (MQ=255)


                                                                             tCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCt                        >  2:3445606/1‑101 (MQ=255)


                                                                             tCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCt                        >  2:4283044/1‑101 (MQ=255)


                                                                               aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcc                      >  1:3650664/1‑101 (MQ=255)


                                                                               aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGAACCTTCAAAAGTCGATcc                      >  2:538843/1‑101 (MQ=255)


                                                                                gCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcac                     >  2:656702/1‑99 (MQ=255)


                                                                                 ccccGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccc                    >  1:1775632/1‑101 (MQ=255)


                                                                                   ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc                  <  2:3517891/101‑1 (MQ=255)


                                                                                    cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                 <  1:4309877/101‑1 (MQ=255)


                                                                                    cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACTCTATGGGAGGCTGGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                 <  2:2233412/101‑1 (MQ=255)


                                                                                    cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                 <  1:4283361/101‑1 (MQ=255)


                                                                                      cccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcgcg               <  2:1088259/101‑1 (MQ=255)


                                                                                         gcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCgggg            <  1:3867104/101‑1 (MQ=255)


                                                                                           tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc          >  1:4199273/1‑101 (MQ=255)


                                                                                           tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCCCGGGGGc          >  1:3539457/1‑101 (MQ=255)


                                                                                            gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg         >  1:3365092/1‑101 (MQ=255)


                                                                                              ctcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTg       >  2:1766305/1‑101 (MQ=255)


                                                                                                cACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTTGGGAGGCTACGCACCTTCAAAAGAGGCTCCGCCCGCGGGGGGGTGGa     >  2:4054196/1‑101 (MQ=37)


                                                                                                cACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGa     >  2:4939704/1‑101 (MQ=255)


                                                                                                 cccAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:932140/100‑1 (MQ=255)


                                                                                                 aCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:3870965/101‑1 (MQ=255)


                                                                                                  ccAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATc   >  2:2151420/1‑101 (MQ=255)


                                                                                                   cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATTGGATGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:3410429/101‑1 (MQ=255)


                                                                                                   cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:3927550/101‑1 (MQ=255)


                                                                                                   cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:4171767/101‑1 (MQ=255)


TGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397800‑1397998

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG