Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,412,658 G→A 100% H391Y (CAT→TAT)  RCAP_rcc01318 ← outer membrane autotransporter barrel domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,412,6580G→A100.0% 733.6 204H391Y (CAT→TAT) RCAP_rcc01318outer membrane autotransporter barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (111/93):  ref base (0/0):  total (111/93)

Only 100 of 211 total aligned reads displayed.

CACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCTCGCGACTTGGGCGTCAACATGGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTGCCA  >  NC_014034/1412560‑1412755
                                                                                                  |                                                                                                 
caccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATagg                                                                                                 >  2:195285/1‑101 (MQ=255)
  ccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                                                                               <  2:1726311/101‑1 (MQ=255)
  ccGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                                                                               <  2:673659/101‑1 (MQ=255)
   cGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCt                                                                                              >  2:3469167/1‑101 (MQ=255)
      gCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGCGGTGCCCACCGCCCCCGCGCCTTGGGCGCCAACACAGCCCTTcc                                                                                           >  1:4919092/1‑100 (MQ=11)
         gTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAgg                                                                                        >  2:184288/1‑101 (MQ=19)
          ttCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGc                                                                                       >  2:3165269/1‑101 (MQ=16)
           tCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                                                                                      >  1:534928/1‑101 (MQ=12)
           tCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                                                                                      >  1:2394612/1‑101 (MQ=12)
            cACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCa                                                                                     >  1:4499425/1‑101 (MQ=9)
            cACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCa                                                                                     >  1:1871432/1‑101 (MQ=9)
            cACTGCGAAAGTGGTGCCAGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCa                                                                                     >  2:3849186/1‑101 (MQ=2)
                gCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTg                                                                                 >  1:3151513/1‑101 (MQ=2)
                  gAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGc                                                                               >  2:2942575/1‑101 (MQ=2)
                   aaaGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGcc                                                                              >  2:4742738/1‑101 (MQ=2)
                              cGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCg                                                                   >  1:4635454/1‑101 (MQ=19)
                              cGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCg                                                                   <  1:1426524/101‑1 (MQ=16)
                              cGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCg                                                                   >  2:3790283/1‑101 (MQ=19)
                               gCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgg                                                                  >  1:3995412/1‑101 (MQ=255)
                               gCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgg                                                                  >  1:2771960/1‑101 (MQ=255)
                               gCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgg                                                                  >  2:263455/1‑101 (MQ=255)
                                cAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCggg                                                                 <  2:2989170/101‑1 (MQ=255)
                                cAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCggg                                                                 >  2:1023589/1‑101 (MQ=255)
                                cAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCggg                                                                 >  2:4974841/1‑101 (MQ=255)
                                 aaGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgggg                                                                >  1:1810777/1‑101 (MQ=255)
                                 aaGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCgggg                                                                >  2:704702/1‑101 (MQ=255)
                                   gagaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGcc                                                              <  2:243000/101‑1 (MQ=255)
                                   gagaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGcc                                                              <  1:5032922/101‑1 (MQ=255)
                                    agaCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCcg                                                             <  2:181590/101‑1 (MQ=255)
                                       cAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCgg                                                          <  1:1738375/101‑1 (MQ=255)
                                       cAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCgg                                                          <  2:767413/101‑1 (MQ=255)
                                        aGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGt                                                         <  2:2618543/101‑1 (MQ=255)
                                           ccGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCgg                                                      >  2:951760/1‑101 (MQ=255)
                                            cGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCggg                                                     >  2:2071826/1‑101 (MQ=255)
                                             gTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGa                                                    >  1:3469803/1‑101 (MQ=255)
                                             gTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGa                                                    >  2:3971585/1‑101 (MQ=255)
                                              tctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAc                                                   >  2:4516257/1‑101 (MQ=255)
                                              tctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAc                                                   >  1:926601/1‑101 (MQ=255)
                                               ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                                                  <  1:1078972/101‑1 (MQ=255)
                                               ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                                                  >  2:743435/1‑101 (MQ=255)
                                               ctcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGAcc                                                  >  1:2065307/1‑101 (MQ=255)
                                                tcCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCg                                                 >  1:3186175/1‑101 (MQ=255)
                                                 cccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGt                                                <  2:444922/101‑1 (MQ=255)
                                                  ccGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTc                                               <  2:2793461/101‑1 (MQ=255)
                                                   cgcgGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCg                                              <  2:2886943/101‑1 (MQ=255)
                                                     cgGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGc                                            >  1:576375/1‑101 (MQ=255)
                                                     cgGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGc                                            >  1:971936/1‑101 (MQ=255)
                                                     cgGCAGAGGTGGCGCCCACCGCGGCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGTCGCGGTCGGGACCGTCGGc                                            <  1:486995/101‑1 (MQ=17)
                                                      ggCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCa                                           >  1:2666862/1‑101 (MQ=255)
                                                      ggCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGGCGGGGGCGCGGTCGGGACCGCCGGCa                                           >  2:3112096/1‑101 (MQ=17)
                                                       gCATAGGTGGTGCCCACCGCGCCCGCGCCTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGCCGGGACCGTCGGCAg                                          >  1:3916169/1‑101 (MQ=17)
                                                       gCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAg                                          >  1:2049410/1‑101 (MQ=255)
                                                        cATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAgg                                         <  2:4923630/101‑1 (MQ=255)
                                                        cATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAgg                                         <  1:3633818/101‑1 (MQ=255)
                                                        cATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAgg                                         >  1:4218516/1‑101 (MQ=255)
                                                         aTAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCCCCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAggg                                        <  1:302160/101‑1 (MQ=255)
                                                         aTAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAggg                                        >  2:2002514/1‑101 (MQ=255)
                                                           aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                                      <  2:1109920/101‑1 (MQ=255)
                                                           aGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGAc                                      >  1:1567897/1‑101 (MQ=255)
                                                            ggtggtGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGAAGGGACg                                     >  1:4842384/1‑101 (MQ=255)
                                                              tggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgag                                   <  1:184304/101‑1 (MQ=255)
                                                              tggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgag                                   <  1:3849471/101‑1 (MQ=255)
                                                               ggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgaga                                  <  2:1132744/101‑1 (MQ=255)
                                                               ggtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACgaga                                  >  1:3099107/1‑101 (MQ=255)
                                                                gtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGaa                                 >  2:3334900/1‑101 (MQ=255)
                                                                gtgCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGCCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGaa                                 >  1:3420019/1‑101 (MQ=255)
                                                                  gCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGACGGCAGGGACGAGAAGt                               >  2:4538449/1‑101 (MQ=255)
                                                                   cccACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtt                              <  2:1567767/101‑1 (MQ=255)
                                                                     cACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtttt                            >  2:4233729/1‑101 (MQ=255)
                                                                     cACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGtttt                            <  1:1676059/101‑1 (MQ=255)
                                                                      aCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGttttt                           <  1:1168224/101‑1 (MQ=255)
                                                                         gcgcCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTcac                        <  2:3494355/101‑1 (MQ=255)
                                                                           gcccgcgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCacac                      >  2:2159886/4‑101 (MQ=255)
                                                                           gcccgcgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCacac                      <  2:1400042/98‑1 (MQ=255)
                                                                           gcccgcgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCacac                      <  2:576296/98‑1 (MQ=255)
                                                                              cgcgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCg                   >  2:1218319/1‑101 (MQ=255)
                                                                               gcgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGc                  <  2:2715831/101‑1 (MQ=255)
                                                                                cgACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGcc                 <  1:775682/101‑1 (MQ=255)
                                                                                 gACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCa                <  2:4320591/101‑1 (MQ=255)
                                                                                 gACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCa                <  1:3602420/101‑1 (MQ=255)
                                                                                 gACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCa                <  1:2501848/101‑1 (MQ=255)
                                                                                  aCTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGCAGTTTTTCACAACCGCCAg               >  2:4163792/1‑101 (MQ=255)
                                                                                   cTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGc              >  1:3574341/1‑101 (MQ=255)
                                                                                   cTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACAACCGCCAGc              >  2:2804523/1‑101 (MQ=255)
                                                                                       ggCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCt          >  2:4403836/1‑101 (MQ=255)
                                                                                       ggCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCt          <  1:1235197/101‑1 (MQ=255)
                                                                                       ggCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCCCCCGCCTcc          >  1:2261357/1‑100 (MQ=37)
                                                                                        gCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTg         <  1:1058904/101‑1 (MQ=255)
                                                                                        atctCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACAAGAAGTTTTTCACACCCGCCAGCGTCTg         <  1:2012818/98‑1 (MQ=255)
                                                                                         cGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGa        <  2:2839105/101‑1 (MQ=255)
                                                                                          gTCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGAt       >  1:1143892/1‑101 (MQ=255)
                                                                                           tCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGAtt      <  1:3231859/101‑1 (MQ=255)
                                                                                           tCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGAtt      >  1:742937/1‑101 (MQ=255)
                                                                                           tCAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGAtt      >  1:2820960/1‑101 (MQ=255)
                                                                                            cAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTg     >  1:559311/1‑101 (MQ=255)
                                                                                            cAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTg     >  1:3687698/1‑101 (MQ=255)
                                                                                            cAACATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACAGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTg     >  1:692330/1‑101 (MQ=255)
                                                                                             aaCATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTGc    <  2:4903954/101‑1 (MQ=255)
                                                                                             aaCATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTGc    >  2:270028/1‑101 (MQ=255)
                                                                                              aCATAGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTGcc   <  2:2012965/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CACCGTGCCGTTCACTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCTCGCGACTTGGGCGTCAACATGGGCCTTCGAGGCCACCTGGCCCGCAAGCGTCGGGGCCGCGGTCGGGACCGTCGGCAGGGACGAGAAGTTTTTCACACCCGCCAGCGTCTGATTGCCA  >  NC_014034/1412560‑1412755

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG