Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,575,647	G→C	100%	intergenic (+49/+124)	corA → / ← RCAP_rcc01455	magnesium/cobalt transport protein CorA/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,575,647	0	G→C	100.0%	121.3	37	intergenic (+49/+124)	corA/RCAP_rcc01455	magnesium/cobalt transport protein CorA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/35):  ref base (0/2):  total (0/37)

CTGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGCCATTTG.CGGCG.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGC  >  NC_014034/1575565‑1575723                                                                                    |                                                                              cTGGGCCTTCTTCAAATGGAAGGTCTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGcc                                                             <  2:3894280/101‑1 (MQ=25) cTGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGcc                                                             <  2:215973/101‑1 (MQ=37)   gggCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTG.CCGCCCCCCGCACCCCCAGCCTg                                                           <  1:1797535/101‑1 (MQ=255)    ggtctttttAAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCGCGCGCGGCTCGGGCCCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGc                                                          <  1:407742/98‑1 (MQ=19)        ttcttcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCgggg                                                      <  2:1750327/101‑1 (MQ=37)          cttcAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGa                                                    <  2:4445467/101‑1 (MQ=37)              aaaTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGAt                                                <  2:435141/101‑1 (MQ=37)                aTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGt                                              <  2:4642934/101‑1 (MQ=37)                aTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGt                                              <  2:4575982/101‑1 (MQ=37)                aTGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGt                                              <  1:2190947/101‑1 (MQ=37)                      ggACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCcg                                        <  1:2476487/101‑1 (MQ=37)                      ggACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCcg                                        <  1:409190/101‑1 (MQ=37)                        gcTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCCCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCcgcg                                      <  1:3570893/100‑1 (MQ=25)                        aCTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCcgcg                                      <  1:3949041/101‑1 (MQ=37)                          tGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGcc                                    <  2:2844861/101‑1 (MQ=37)                           ggCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGccc                                   <  2:347712/101‑1 (MQ=37)                           ggCTTTAGGGCTGGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGccc                                   <  2:1835066/101‑1 (MQ=25)                              tttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCt                                <  1:117725/101‑1 (MQ=37)                               ttAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCtt                               <  2:3109199/101‑1 (MQ=37)                                tAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTg                              <  1:4450607/101‑1 (MQ=37)                                 aGGGCTTGCTCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGc                             <  1:2506211/101‑1 (MQ=25)                                 aGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGc                             <  2:4757535/101‑1 (MQ=37)                                 aGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGc                             <  2:1130986/101‑1 (MQ=37)                                 aGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGc                             <  1:4981234/101‑1 (MQ=37)                                 aGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGc                             <  1:4571955/101‑1 (MQ=37)                                      ttGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTAtt                        <  1:3339557/101‑1 (MQ=37)                                       tGCGCCCGCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTAttt                       <  2:1551234/101‑1 (MQ=25)                                         cgcCCTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTgc                     <  1:1308550/101‑1 (MQ=37)                                             cTCGCGCGGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCa                 <  1:125616/101‑1 (MQ=37)                                                gcgcgGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaaga              <  1:1416964/101‑1 (MQ=37)                                                gcgcgGCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaaga              <  1:1357138/101‑1 (MQ=37)                                                  gcgGCTCGGGCGCCCGGAACGCGGGGGCCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaagaag            <  2:1629934/101‑1 (MQ=255)                                                  gcgGCTCGGGCGCCCGGAACCCGGGGGGCTGCCGCCC.CCCCCGCCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCaagaag            <  1:4836548/101‑1 (MQ=255)                                                     gCTCGGGCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCCCCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGaaa         <  1:3559687/101‑1 (MQ=255)                                                          ggCGCCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCt    <  2:2638771/101‑1 (MQ=37)                                                           gcgcCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTg   <  1:4859222/101‑1 (MQ=37)                                                           gcgcCCGGAACGCGGGGGGCTGCCGCCC.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTg   <  1:1200739/101‑1 (MQ=37)                                                            cgcCCGGAACGCGGGGGGCTG.CCGCCCCCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGc  <  1:3777775/101‑1 (MQ=37)                                                                                    |                                                                              CTGGGCCTTCTTCAAATGGAAGGACTGGCTTTAGGGCTTGCGCCCTCGCGCGGCTCGGGCGCCCGGAACGCGGCCATTTG.CGGCG.CCCGCACCCCCCGCCTGCGGGGGACGATGTCCCCCGCGCCCCCTTGCGTATTTGCGCCAAGAAGAAACCCCTGC  >  NC_014034/1575565‑1575723 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG