Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,894 C→A 4.0% intergenic (‑147/+46) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8940C→A 4.0% 15.4 ‑3.6 ‑0.1272intergenic (‑147/+46)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/2):  ref base (120/141):  total (129/143)
Fisher's exact test strand distribution p-value = 2.82e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.04e-01

Only 100 of 292 total aligned reads displayed.

CAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCG  >  NC_014034/1734795‑1734994
                                                                                                      |                                                                                                     
 aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                                      >  2:2509406/1‑101 (MQ=255)
  aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                     >  2:1745892/1‑101 (MQ=255)
  aaGGCTGACCCTGCGTCAGGCGGCGGGCCGGGGAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                     <  1:1849939/101‑1 (MQ=17)
    ggCGGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                   <  2:2324996/101‑1 (MQ=255)
     gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                  <  2:2816641/101‑1 (MQ=255)
     gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                  <  1:1968823/101‑1 (MQ=255)
     gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                  <  1:3973982/101‑1 (MQ=255)
      cTGGCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                                 <  2:4350075/101‑1 (MQ=255)
      cTGACGCTGTGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                                 >  1:4595161/1‑101 (MQ=255)
      cTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                                 <  1:719803/101‑1 (MQ=255)
          cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                             <  1:1107300/101‑1 (MQ=255)
           gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                            <  1:4669641/101‑1 (MQ=255)
            cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                           <  1:1029484/101‑1 (MQ=255)
                gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                       <  2:923559/101‑1 (MQ=255)
                gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                       >  2:4425896/1‑101 (MQ=255)
                 tgaGCCGAGGGGCCGGGCAGGGGGGCGCTGG.CCTCCGTCCTGGGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                      <  2:308478/99‑1 (MQ=17)
                   aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                    <  2:1681254/101‑1 (MQ=255)
                    gCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                   <  1:603564/101‑1 (MQ=255)
                     ccGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                  <  1:293220/101‑1 (MQ=255)
                     ccGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                  <  2:1374481/101‑1 (MQ=255)
                      cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCCTCTTGCCATCAATATcc                                                                                 >  2:771510/1‑101 (MQ=14)
                       gACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                >  1:2161940/1‑101 (MQ=255)
                        aCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                              <  2:3273151/101‑1 (MQ=255)
                            tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGTGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                         <  1:4969638/93‑1 (MQ=0)
                            tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                         >  2:2769197/9‑101 (MQ=2)
                             gacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                        >  2:1943148/8‑100 (MQ=2)
                             gacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                        >  2:2594548/8‑100 (MQ=2)
                             gacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                        >  1:4781948/8‑100 (MQ=2)
                             ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                         >  2:2206897/1‑101 (MQ=255)
                             ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                         <  2:242560/101‑1 (MQ=255)
                              cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                        <  1:1296181/101‑1 (MQ=255)
                               gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                       <  1:1206218/101‑1 (MQ=255)
                               cggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                      >  1:3231577/6‑98 (MQ=2)
                                   aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                   <  1:3407255/101‑1 (MQ=255)
                                   aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGc                                                                   >  2:1114058/1‑101 (MQ=255)
                                     gggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                 >  2:822347/1‑101 (MQ=255)
                                     gggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                 <  2:1527743/101‑1 (MQ=255)
                                     gggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                 <  2:4237567/101‑1 (MQ=255)
                                      ggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                                <  1:411552/101‑1 (MQ=255)
                                        ggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                              <  2:4826812/101‑1 (MQ=255)
                                        ggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                              <  1:3900749/101‑1 (MQ=255)
                                        ggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                              >  1:3685804/1‑101 (MQ=255)
                                          cgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                            <  1:4903257/101‑1 (MQ=255)
                                            gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                           >  1:4451699/6‑101 (MQ=255)
                                            gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                           <  2:199661/96‑1 (MQ=255)
                                             ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                          >  1:3354755/5‑101 (MQ=255)
                                              tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGAGCCCCGc                                                         >  2:750858/4‑101 (MQ=255)
                                                 cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                      >  2:706784/1‑101 (MQ=255)
                                                   cccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                                    <  1:2315326/101‑1 (MQ=255)
                                                     cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                  <  1:258258/101‑1 (MQ=255)
                                                      ctcttccgatcTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                 <  1:3809531/91‑1 (MQ=255)
                                                       tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                <  2:3890689/101‑1 (MQ=255)
                                                       tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAATGC.GGCCTTCATCTTGCCATAAATATACC.GGGGTGCGGGGCAGAGCCCCGccccaaccaa                                                >  2:1086209/1‑92 (MQ=21)
                                                          tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                             <  1:3461814/101‑1 (MQ=255)
                                                          tGCGGACGCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                             <  1:1510091/101‑1 (MQ=255)
                                                           gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                            <  1:2238455/101‑1 (MQ=255)
                                                            cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                           >  2:103686/1‑101 (MQ=255)
                                                             ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                          >  1:2012055/1‑101 (MQ=255)
                                                             ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                          <  1:2402227/101‑1 (MQ=255)
                                                              gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                         <  2:1576405/101‑1 (MQ=255)
                                                              gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                         <  1:914096/101‑1 (MQ=255)
                                                                  ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGTCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                     <  2:865956/101‑1 (MQ=255)
                                                                  ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAtcggaagag                                     >  2:3809246/1‑93 (MQ=255)
                                                                  ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                     <  2:67229/101‑1 (MQ=255)
                                                                         ggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                              >  1:3707010/1‑101 (MQ=255)
                                                                          gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                             >  1:3556518/1‑101 (MQ=255)
                                                                          gATATTAAGGGAAAGATGAAACTGC.GGCCTTGATCTTGCCATAAATATCCG.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                             <  2:3564982/101‑1 (MQ=37)
                                                                            tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                           >  2:3346786/1‑101 (MQ=255)
                                                                            tatTGATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                           <  2:2637414/101‑1 (MQ=255)
                                                                             atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                          >  1:2518934/1‑101 (MQ=255)
                                                                             atTTATGGCAAGATGAAACGGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                          <  2:3460254/101‑1 (MQ=255)
                                                                              tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                         >  2:1480333/1‑101 (MQ=255)
                                                                               ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                        >  1:4729978/1‑101 (MQ=255)
                                                                                tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                       <  2:4004375/101‑1 (MQ=255)
                                                                                 aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATTTCCC.GTGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCggacggggggc                      <  2:2851040/101‑10 (MQ=37)
                                                                                 aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                      >  2:4270793/1‑101 (MQ=255)
                                                                                  tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCTGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                     >  1:4242254/1‑101 (MQ=255)
                                                                                  tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                     >  1:1460102/1‑101 (MQ=255)
                                                                                  tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                     <  2:1262467/101‑1 (MQ=255)
                                                                                  tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                     <  1:166766/101‑1 (MQ=255)
                                                                                  tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                     <  2:2310389/101‑1 (MQ=255)
                                                                                      aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                 >  2:1874554/1‑101 (MQ=255)
                                                                                      aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                 >  2:132855/1‑101 (MQ=255)
                                                                                        gATGACACTGC.GGCCTTCGTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat               >  2:2120395/1‑101 (MQ=255)
                                                                                        gATGAAGCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat               <  2:4594839/101‑1 (MQ=255)
                                                                                        gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat               <  2:505700/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCTTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAATATCCGCGGCCCGGTTTTCGCtgatg              <  2:4644030/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg              <  1:3748588/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg              <  1:1930480/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg              <  2:313812/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg              <  2:4690525/101‑1 (MQ=255)
                                                                                         aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg              >  1:3055065/1‑101 (MQ=255)
                                                                                          tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga             >  2:4152610/1‑101 (MQ=255)
                                                                                          tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga             <  1:3191177/101‑1 (MQ=255)
                                                                                            aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGGGc           >  2:4986446/1‑101 (MQ=255)
                                                                                            aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc           >  1:1613082/1‑101 (MQ=255)
                                                                                             aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa          <  2:3706932/101‑1 (MQ=255)
                                                                                              aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa         >  2:3371863/1‑101 (MQ=255)
                                                                                                tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc       <  1:328546/101‑1 (MQ=255)
                                                                                                tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc       <  1:4884835/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCG  >  NC_014034/1734795‑1734994

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG