Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,908 T→G 3.7% intergenic (‑161/+32) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,9080T→G 3.7% 15.9 ‑3.9 ‑0.0273intergenic (‑161/+32)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/1):  ref base (130/133):  total (139/134)
Fisher's exact test strand distribution p-value = 1.95e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 294 total aligned reads displayed.

GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008
                                                                                                     |                                                                                                     
gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                       >  1:2521687/1‑101 (MQ=255)
  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                                     <  2:923559/101‑1 (MQ=255)
    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                                   >  1:2236658/1‑101 (MQ=255)
     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAGCTGC.GGCCTTCATCTTGCCATAAata                                                                                                  >  1:4237884/1‑101 (MQ=255)
     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                                  <  1:1768802/101‑1 (MQ=255)
     aGCCGACGGCCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                                  <  2:1734777/101‑1 (MQ=255)
      gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                                 <  1:603564/101‑1 (MQ=255)
        cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCCTCTTGCCATCAATATcc                                                                                               >  2:771510/1‑101 (MQ=14)
        cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                               <  1:3405444/101‑1 (MQ=255)
        cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                               <  1:3646866/101‑1 (MQ=255)
        cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                               >  1:1978683/1‑101 (MQ=255)
         gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATTGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                              >  2:2562052/1‑101 (MQ=255)
          gcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                            <  1:4086673/100‑1 (MQ=255)
          aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                            <  2:3273151/101‑1 (MQ=255)
           cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                                           <  2:1939106/101‑1 (MQ=255)
             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                                         >  1:3090263/1‑101 (MQ=255)
             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                                         <  1:2549031/101‑1 (MQ=255)
              tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGTGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                                       <  1:4969638/93‑1 (MQ=0)
               gacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                                      >  1:4662485/8‑100 (MQ=2)
               gacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                                      >  1:4781948/8‑100 (MQ=2)
               ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                                       >  2:2206897/1‑101 (MQ=255)
                 cggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                                    >  1:3053759/6‑98 (MQ=2)
                 cggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                                    >  2:4681000/6‑98 (MQ=2)
                    cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATATTGCCATAAATATCCC.GGGGTGCgggg                                                                                  >  1:3911733/1‑101 (MQ=255)
                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                                >  1:770825/1‑101 (MQ=255)
                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                                >  2:1147126/1‑101 (MQ=255)
                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                                >  2:437531/1‑101 (MQ=255)
                       gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                               <  2:1527743/101‑1 (MQ=255)
                       gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                               >  2:822347/1‑101 (MQ=255)
                        ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATTGCAAGATTAAACTGC.GGCTTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                                              >  2:3352244/1‑101 (MQ=17)
                          ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                                            <  2:4826812/101‑1 (MQ=255)
                            cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                                          <  1:4903257/101‑1 (MQ=255)
                             gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                         <  1:2674244/96‑1 (MQ=255)
                              ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                        >  2:3318036/5‑101 (MQ=255)
                               tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGAGCCCCGc                                                                       >  2:750858/4‑101 (MQ=255)
                                tccccccTTCCTGCGGACTCCCCCCTGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                      <  2:4996964/99‑1 (MQ=255)
                                  cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                    >  2:706784/1‑101 (MQ=255)
                                  cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                    <  1:344207/101‑1 (MQ=255)
                                     ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                                 >  1:1251987/1‑101 (MQ=255)
                                      cGTCCTGCGGGCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCTCTCAACCg                                                                <  1:2017646/101‑1 (MQ=255)
                                       ctcttccgatcTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                               <  1:3809531/91‑1 (MQ=255)
                                        tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCG.CGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                              <  1:4497692/101‑1 (MQ=255)
                                        tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                              <  2:3890689/101‑1 (MQ=255)
                                        tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAATGC.GGCCTTCATCTTGCCATAAATATACC.GGGGTGCGGGGCAGAGCCCCGccccaaccaa                                                              >  2:1086209/1‑92 (MQ=21)
                                         ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                                             >  2:4530620/1‑101 (MQ=255)
                                         ccTGCGGACCCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                                             <  2:1392983/101‑1 (MQ=255)
                                             cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                         >  2:103686/1‑101 (MQ=255)
                                              ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                        <  1:1305450/101‑1 (MQ=255)
                                               gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTCCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                                       <  1:933511/101‑1 (MQ=255)
                                                 cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                                     <  1:4326861/101‑1 (MQ=255)
                                                   ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGTCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                   <  2:865956/101‑1 (MQ=255)
                                                   ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAtcggaagag                                                   >  2:3809246/1‑93 (MQ=255)
                                                   ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                   <  2:67229/101‑1 (MQ=255)
                                                   ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                   <  2:2755956/101‑1 (MQ=255)
                                                       ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               >  2:2390970/1‑101 (MQ=255)
                                                       ccGGGATATTTATGGCAAGATGAAACTGC.GGACTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               <  2:3086194/101‑1 (MQ=255)
                                                        cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                                              >  1:239872/1‑101 (MQ=255)
                                                          ggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                                            >  1:3707010/1‑101 (MQ=255)
                                                           gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  1:3556518/1‑101 (MQ=255)
                                                           gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           >  1:3252441/1‑101 (MQ=255)
                                                           gATATTAAGGGAAAGATGAAACTGC.GGCCTTGATCTTGCCATAAATATCCG.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                           <  2:3564982/101‑1 (MQ=37)
                                                             tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         >  2:4241207/1‑101 (MQ=255)
                                                             tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGCCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         >  1:1479410/1‑101 (MQ=255)
                                                             tatTGATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                         <  2:2637414/101‑1 (MQ=255)
                                                              atTTATGGCAAGATGAAACGGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                                        <  2:3460254/101‑1 (MQ=255)
                                                               tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  2:1480333/1‑101 (MQ=255)
                                                                 tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     <  2:4004375/101‑1 (MQ=255)
                                                                 tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                     <  2:281560/101‑1 (MQ=255)
                                                                  aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATTTCCC.GTGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCggacggggggc                                    <  2:2851040/101‑10 (MQ=37)
                                                                  aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                    >  2:4270793/1‑101 (MQ=255)
                                                                  aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                                    >  2:4610522/1‑101 (MQ=255)
                                                                   tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                   <  2:2310389/101‑1 (MQ=255)
                                                                      cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                                >  2:4693612/1‑101 (MQ=255)
                                                                         gATGACACTGC.GGCCTTCGTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             >  2:2120395/1‑101 (MQ=255)
                                                                          aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            <  1:1930480/101‑1 (MQ=255)
                                                                          aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            <  1:3748588/101‑1 (MQ=255)
                                                                           tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga                           <  1:1114172/101‑1 (MQ=255)
                                                                            gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                          >  2:1869908/1‑101 (MQ=255)
                                                                             aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                         >  1:1613082/1‑101 (MQ=255)
                                                                              aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  2:3706932/101‑1 (MQ=255)
                                                                              aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        >  2:2482947/1‑101 (MQ=255)
                                                                                 tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                     <  1:4884835/101‑1 (MQ=255)
                                                                                     ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                  >  1:4734712/1‑101 (MQ=255)
                                                                                      gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                 >  1:3209119/1‑101 (MQ=255)
                                                                                        cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               <  1:3347191/101‑1 (MQ=255)
                                                                                        cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               >  2:1353593/1‑101 (MQ=255)
                                                                                        cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               >  2:1250068/1‑101 (MQ=255)
                                                                                         ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  2:3227779/101‑1 (MQ=255)
                                                                                         ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              >  2:1981220/1‑101 (MQ=255)
                                                                                          tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             >  1:1510426/1‑101 (MQ=255)
                                                                                            aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           <  2:1603734/101‑1 (MQ=255)
                                                                                            aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           >  1:3873923/1‑101 (MQ=255)
                                                                                              cTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa         >  1:1037531/1‑101 (MQ=255)
                                                                                              cTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAACCGAAGATGCCAAGATCCGCGGCCCGGTCTTCGCTGATAAGCAAGCGCCCGTTCGGCa         >  1:2876588/1‑101 (MQ=38)
                                                                                                tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       <  1:2308876/101‑1 (MQ=255)
                                                                                                tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  1:1650829/1‑101 (MQ=255)
                                                                                                  ccATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc     >  1:3315382/1‑101 (MQ=255)
                                                                                                   cATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCg    <  1:2457944/101‑1 (MQ=255)
                                                                                                    aTAAATATCCA.GGGGTGCGGGGCGGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGt   <  2:2003292/101‑1 (MQ=255)
                                                                                                     tAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt  >  2:604199/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG