Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 G→C 97.4% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7850G→C97.4% 4.0 ‑3.5 ‑1.0230intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (109/115):  ref base (0/6):  total (109/121)
Fisher's exact test strand distribution p-value = 3.05e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-02

Only 100 of 244 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GA.GG.GG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880
                                                                                                    |                                                                                                
gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAg                                                                                             >  1:4071554/1‑101 (MQ=255)
gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAg                                                                                             >  1:382742/1‑101 (MQ=255)
   gtttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCg                                                                                          <  2:2899774/99‑1 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  1:4998322/1‑101 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  2:1853033/1‑101 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  2:2542427/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGcc                                                                                     >  1:2481117/1‑101 (MQ=255)
         aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCt                                                                                    <  1:2350214/101‑1 (MQ=255)
          gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTc                                                                                   <  2:4762237/101‑1 (MQ=255)
           ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCg                                                                                  <  1:3556408/101‑1 (MQ=255)
            cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCgg                                                                                 >  2:400942/1‑101 (MQ=255)
            cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCCGCCCCCGC.CGAGGCGATGCCTCgg                                                                                 >  2:2749568/1‑101 (MQ=255)
              aCGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                               >  1:4692881/1‑101 (MQ=255)
              aCGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GA.GG.GGCCGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                               >  1:339913/1‑101 (MQ=255)
               cGGAAGACCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              <  1:33319/101‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              >  1:3686724/1‑101 (MQ=255)
                 gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGGTGCCTCGGcccgc                                                                            >  2:1650838/1‑99 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTGCCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  2:4910431/101‑1 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  1:83615/101‑1 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  2:517621/101‑1 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:206526/1‑101 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:4743579/1‑101 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:1191723/1‑101 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  1:3980412/101‑1 (MQ=255)
                       ccGCTTCTGGACTATCTCGAAAGCAAATGCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                      <  1:4450752/101‑1 (MQ=255)
                       ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                      <  2:90843/101‑1 (MQ=255)
                         gCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  2:571501/101‑1 (MQ=37)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  1:1900399/101‑1 (MQ=38)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCCC.CGAGGCGATGCCTCGGCCCCCCGGGattt                                                                    >  1:2465843/1‑99 (MQ=255)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   <  2:35973/101‑1 (MQ=38)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   <  1:1620350/101‑1 (MQ=38)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  2:3742499/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:745247/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                <  1:4608126/101‑1 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               >  1:541119/1‑101 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GA.GG.GGTCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               <  2:1083638/101‑1 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  1:2146330/1‑101 (MQ=38)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  1:4396854/1‑101 (MQ=38)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  2:709305/1‑101 (MQ=38)
                                       tcGAAAGCAAATCCGCCGCGATCTACCGGCTTGGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      <  1:991067/101‑1 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      >  1:1295697/1‑101 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      >  1:2834208/1‑101 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCGCGGCCCCACGGGATATTTAGatcggaagag                                                      >  2:835218/1‑92 (MQ=255)
                                       ctgAAAGCAAATCCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      <  2:4123767/99‑1 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGCTATTTCGAGAAAGATGaa                                                     >  2:3556007/1‑101 (MQ=255)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     <  1:3856340/101‑1 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     <  2:1692953/101‑1 (MQ=37)
                                         gAAAGCAAATCCCCCGCGGTCTGCGGGCTTGGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    <  1:4178033/101‑1 (MQ=21)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.GGCTGCCCCCGC.CTAGGCGATGCCTCGGCCCCCCGGGGGATTTCTAGGAAGAGGaaa                                                    >  1:490612/1‑101 (MQ=255)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:1162093/1‑101 (MQ=37)
                                          aaaGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   >  2:1963089/1‑101 (MQ=37)
                                              cAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  1:1777986/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  2:1726795/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               >  2:2150861/1‑101 (MQ=37)
                                               aaaTCCCCCGCGATCTACCGGCTTGGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              <  2:1740040/101‑1 (MQ=25)
                                               aaaTACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGTTGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  1:3130717/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  2:3173683/1‑101 (MQ=37)
                                                aaTACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:975947/1‑101 (MQ=37)
                                                 aTACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            >  2:3869973/1‑101 (MQ=37)
                                                  tACGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           >  2:2044418/1‑101 (MQ=37)
                                                   aCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                                          >  1:2041057/1‑101 (MQ=37)
                                                   aCGCCGCGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                                          >  1:2424165/1‑101 (MQ=37)
                                                     gccgcGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  2:1383760/101‑1 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCNNGnntn                                       >  1:1754314/1‑100 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       >  1:1039955/1‑101 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       <  1:2281694/101‑1 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       <  2:2449692/101‑1 (MQ=37)
                                                       cgcgATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCa                                      <  1:401007/101‑1 (MQ=37)
                                                        gcttttttccGCCTTTGA.CGGC.GA.GGTGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                     <  2:2405366/93‑1 (MQ=21)
                                                        gcgATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                     >  1:1502489/1‑101 (MQ=37)
                                                          gATCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   >  1:5020973/1‑101 (MQ=37)
                                                           ttCTACCGGCTTTGG.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:4700847/100‑1 (MQ=25)
                                                           aTCTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGAAATTTCGAGCAAAATGAAAAGCCTCCGGTTTAATCtt                                  >  1:2548279/1‑101 (MQ=255)
                                                            tCTGCCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 <  1:2657641/101‑1 (MQ=25)
                                                            tCTCCCGGCTTTGA.GGGCGGG.GG.GG.CGC.GCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 <  1:3447684/101‑1 (MQ=21)
                                                             cTACCGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                >  2:4789184/1‑101 (MQ=25)
                                                                ccGGCTTTGAGGGGG.GG.GG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  1:2159353/101‑1 (MQ=21)
                                                                ccGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             >  2:2813700/1‑101 (MQ=25)
                                                                 cGGCTTTGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCCGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            >  2:2618459/1‑101 (MQ=25)
                                                                 cGGCTTGGG.GGGCGGG.GG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            <  2:4066632/101‑1 (MQ=21)
                                                                       tGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  2:3809004/1‑101 (MQ=25)
                                                                       tGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  2:5019848/1‑101 (MQ=25)
                                                                       tGA.GGGC.GAGGG.GG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  2:4256990/1‑101 (MQ=25)
                                                                               cgaggg.gg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggg                >  1:2795889/5‑101 (MQ=255)
                                                                                gaggg.gg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:1090660/4‑101 (MQ=255)
                                                                                gaggg.gg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               <  2:3923090/98‑1 (MQ=255)
                                                                                 gggg.gt.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              <  2:3208576/99‑1 (MQ=255)
                                                                                 aggg.gg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              >  2:4514249/3‑101 (MQ=255)
                                                                                    g.gg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  2:672853/101‑1 (MQ=255)
                                                                                      gg.CGCTGCCCCAGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTc          >  2:1775901/1‑101 (MQ=255)
                                                                                       g.cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  2:4995523/101‑1 (MQ=255)
                                                                                       g.cgcTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  1:3733219/101‑1 (MQ=255)
                                                                                        gcgtttcccccgC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  2:2658547/95‑1 (MQ=18)
                                                                                         cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        >  1:1348182/1‑101 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGCGTCCCg       >  2:1540838/1‑101 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  2:3538465/101‑1 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  2:3075875/1‑101 (MQ=255)
                                                                                              cccccgC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   <  2:1502359/101‑1 (MQ=255)
                                                                                                ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  1:701513/101‑1 (MQ=255)
                                                                                                ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  2:3731117/101‑1 (MQ=255)
                                                                                                    |                                                                                                
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGA.GGGC.GA.GG.GG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG