Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,184 T→A 74.8% S661T (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1840T→A74.8% 180.5 ‑0.7 0.0202S661T (TCA→ACA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (73/78):  ref base (28/23):  total (101/101)
Fisher's exact test strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275
                                                                                          |                                                                                            
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   >  2:4150326/1‑101 (MQ=255)
 gtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  <  2:4465801/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  1:2989786/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  2:2944440/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  2:2404380/101‑1 (MQ=255)
   gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaa                                                                                <  1:808480/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaaa                                                                               >  2:259367/1‑101 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  2:1459633/101‑1 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  2:1389054/101‑1 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAg                                                                              <  2:4949167/101‑1 (MQ=255)
      gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGc                                                                             <  1:2381601/101‑1 (MQ=255)
      gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGc                                                                             >  1:58470/1‑101 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGcc                                                                            <  2:1616757/101‑1 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGcc                                                                            <  2:3291655/101‑1 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGcc                                                                            <  1:4894549/101‑1 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGcc                                                                            <  2:4205474/101‑1 (MQ=255)
           cgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtg                                                                        >  1:4503027/1‑101 (MQ=255)
           cgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtg                                                                        >  1:4150703/1‑101 (MQ=255)
            gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ACCCATGACGCCAAAAGCCGCtgt                                                                       >  1:2440375/1‑101 (MQ=255)
                cgcCTATGGCGCGATCAAGCGCACGGGCGAGATCCGCTATTCCGAGCGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGCCGACAAAAGCCGCtccgggc                                                                   >  1:3220768/1‑95 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  1:3651419/101‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  2:2012966/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  1:3333839/101‑1 (MQ=255)
                     aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              >  1:3034395/1‑101 (MQ=255)
                      tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             <  1:3313441/101‑1 (MQ=255)
                        gcgcGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTg                                                           >  1:4863273/1‑101 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         <  1:1010420/101‑1 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         >  2:4812267/1‑101 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         <  2:3194800/101‑1 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         <  1:1583848/101‑1 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     >  1:1131492/1‑101 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     >  1:2586082/1‑101 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  1:3976082/1‑101 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  1:1726461/1‑101 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  1:4587224/101‑1 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  2:3038740/101‑1 (MQ=255)
                                 aGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTATGGCGCTGgcggcg                                                  >  1:4357658/1‑101 (MQ=255)
                                    gcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              >  2:1990985/1‑100 (MQ=255)
                                    gcACCGGCGAGATCCGCTATTCCGAGAGGTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACCACAAAAGCCGCTGTGTGCCTTTGCTGCTGGCGGgg.aca                                              >  1:1924510/1‑99 (MQ=255)
                                      aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            >  1:1239163/1‑98 (MQ=255)
                                      aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            >  2:4547686/1‑98 (MQ=255)
                                      aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGGGCCTTTGGTGCTGGCGGcg.aaaag                                            >  1:2551192/1‑98 (MQ=255)
                                       ccGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.agcggc                                           <  2:2821851/101‑3 (MQ=255)
                                       ccGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacga                                           >  2:4008511/1‑97 (MQ=255)
                                        cGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          <  2:879773/101‑6 (MQ=255)
                                          gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         <  2:2440201/101‑1 (MQ=255)
                                          gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         >  1:3477656/1‑101 (MQ=255)
                                          gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         <  1:3708071/101‑1 (MQ=255)
                                            gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       <  2:2128984/101‑1 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGcc                                      >  2:1511242/1‑100 (MQ=255)
                                             agaTCCGCTATTCCGAGTGCTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      <  2:3451389/101‑1 (MQ=255)
                                                  ctcTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 <  1:2420779/99‑1 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCa                                >  2:3771382/1‑101 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCa                                <  2:4689324/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  2:2998368/1‑101 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  2:1137535/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:3732516/101‑1 (MQ=255)
                                                      aTTGCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:4280804/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:3694202/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:2724927/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:964748/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          >  1:4990097/1‑101 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          <  1:2899229/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          <  1:1521023/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          <  1:3118528/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgc                          <  1:90206/101‑1 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  1:2276490/1‑101 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  2:389234/1‑101 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  1:1972915/1‑101 (MQ=255)
                                                           gAGCGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgtgg                        >  2:1105511/1‑101 (MQ=255)
                                                            aGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggc                       <  1:4281121/101‑1 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      >  2:54589/1‑101 (MQ=255)
                                                               gtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    <  1:389287/101‑1 (MQ=255)
                                                                tttCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggcg                   <  2:30927/101‑1 (MQ=255)
                                                                 ttCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgg                  >  1:4119818/1‑101 (MQ=255)
                                                                  tCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGt                 <  1:98423/101‑1 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                >  1:1366220/1‑101 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTc              >  1:653694/1‑101 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             >  2:619706/1‑101 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  1:2135369/101‑1 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  2:3946826/101‑1 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            <  1:4421931/101‑1 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            >  2:4412317/1‑101 (MQ=255)
                                                                        gCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           >  1:2663762/1‑101 (MQ=255)
                                                                        gCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGACATGAAGCGGCGGCGGGTTCTGc           >  2:2035831/1‑101 (MQ=255)
                                                                           ttGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        >  2:1339751/1‑101 (MQ=255)
                                                                           ttGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        <  2:350227/101‑1 (MQ=255)
                                                                           ttGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGa        >  1:506265/1‑101 (MQ=255)
                                                                            tGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:2194738/1‑101 (MQ=255)
                                                                              aCTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCg     <  1:3539101/101‑1 (MQ=255)
                                                                              aCTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCg     >  2:2215015/1‑101 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    >  1:475037/1‑101 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    >  1:566410/1‑101 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGATCgg    >  2:4237692/1‑100 (MQ=255)
                                                                               cTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCAGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGATCGt    >  2:2324607/1‑101 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTg   >  2:815913/1‑101 (MQ=255)
                                                                                tgtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAGGAAGCGGCGGTGGTTTCGGCAGATCGTg   >  1:1442832/1‑101 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  <  1:3785009/101‑1 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  >  1:1410808/1‑101 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  >  2:1744130/1‑101 (MQ=255)
                                                                                          |                                                                                            
TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG