Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,866,132 G→A 5.6% G489G (GGC→GGT)  RCAP_rcc01729 ← pyridine nucleotide‑disulfide oxidoreductase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,866,1320G→A 5.6% 25.4 ‑4.0 ‑0.1230G489G (GGC→GGT) RCAP_rcc01729pyridine nucleotide‑disulfide oxidoreductase
Reads supporting (aligned to +/- strand):  new base (11/2):  ref base (104/112):  total (115/115)
Fisher's exact test strand distribution p-value = 1.89e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.38e-01

Only 100 of 243 total aligned reads displayed.

TGCATTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCGGCCAGCC  >  NC_014034/1866032‑1866232
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tggaTTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTgg                                                                                                      <  2:4128741/98‑1 (MQ=255)
  cttttCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGcc                                                                                                    <  1:4231143/99‑1 (MQ=255)
  cATTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGcc                                                                                                    >  1:983267/1‑101 (MQ=255)
   aTTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCg                                                                                                   >  1:1116294/1‑101 (MQ=255)
   aTTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCg                                                                                                   <  1:3511045/101‑1 (MQ=255)
    tttCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGc                                                                                                  <  2:3441673/101‑1 (MQ=255)
     ttCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCa                                                                                                 >  2:1165257/1‑101 (MQ=255)
       cGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAgg                                                                                               >  2:5006341/1‑101 (MQ=255)
       cGTCAGCTCGATGCGATAGCCGCGCCCGGGGCCGGGCTTGACGATGCCCTGTTCCGGGCAGGCGGCAAAGCAGTTTTCGCATTCATACACCTGGCCGCAgg                                                                                               >  2:2134759/1‑101 (MQ=255)
        gTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCCGGCGGAAAAGCAGTTGTCGCCTTCATCGAACTGGCCCCAGGa                                                                                              >  2:89008/1‑101 (MQ=255)
        gTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGa                                                                                              <  1:237675/101‑1 (MQ=255)
        gTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATCCATAGCACTGGCCGCAGGa                                                                                              <  2:4726705/101‑1 (MQ=255)
         tCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGaa                                                                                             <  2:239493/101‑1 (MQ=255)
          cAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAAt                                                                                            <  1:78741/101‑1 (MQ=255)
          cAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAAt                                                                                            >  2:198851/1‑101 (MQ=255)
           aGCTCGAGGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATa                                                                                           <  1:946473/101‑1 (MQ=255)
            gCTCGATGTGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGACCGCAGGAATag                                                                                          >  1:4504677/1‑101 (MQ=255)
             cTCGATGCGATAGCCGCGGCGGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACAGGCCGCAGGAATagc                                                                                         <  2:487056/101‑1 (MQ=255)
               cGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATagcag                                                                                       >  1:3995663/1‑101 (MQ=255)
               cGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATagcag                                                                                       <  2:1604805/101‑1 (MQ=255)
                gATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATagcagc                                                                                      <  2:1572889/101‑1 (MQ=255)
                gATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATagcagc                                                                                      >  2:1278566/1‑101 (MQ=255)
                gATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGACCGCAGGAATagcagc                                                                                      >  2:1681908/1‑101 (MQ=255)
                    cGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTg                                                                                  >  1:4998038/1‑101 (MQ=255)
                     gATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTgc                                                                                 <  1:4653925/101‑1 (MQ=255)
                     gATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTgc                                                                                 >  1:1380722/1‑101 (MQ=255)
                     gATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTgc                                                                                 >  1:1720133/1‑101 (MQ=255)
                      aTAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGACCGCAGGAATAGCAGCGTTgcg                                                                                >  2:2084707/1‑101 (MQ=255)
                       tAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTgcgc                                                                               >  1:2034038/1‑101 (MQ=255)
                       tAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTgcgc                                                                               <  1:1946342/101‑1 (MQ=255)
                        aGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGcc                                                                              >  1:2698256/1‑101 (MQ=255)
                         gCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCt                                                                             >  2:3641917/1‑101 (MQ=255)
                           cgcgGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCg                                                                           <  2:3737224/101‑1 (MQ=255)
                            ctggCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGt                                                                          <  2:2499812/99‑1 (MQ=255)
                             cgGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCTTTGCGCCTCGTa                                                                         <  1:2739224/101‑1 (MQ=255)
                             cgGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTa                                                                         <  2:421275/101‑1 (MQ=255)
                             cgGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTa                                                                         <  1:4411272/101‑1 (MQ=255)
                             cgGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTa                                                                         <  1:2994343/101‑1 (MQ=255)
                              ggCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAg                                                                        >  2:2121769/1‑101 (MQ=255)
                              ggCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAg                                                                        <  2:2222507/101‑1 (MQ=255)
                              ggCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAg                                                                        >  1:2146137/1‑101 (MQ=255)
                                       cGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtct                                                               >  1:1653069/1‑101 (MQ=255)
                                       cGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtct                                                               <  1:547632/101‑1 (MQ=255)
                                        gTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtctc                                                              >  2:4774817/1‑101 (MQ=255)
                                          gCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtctctc                                                            <  2:2010953/101‑1 (MQ=255)
                                          gCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtctctc                                                            <  1:3703182/101‑1 (MQ=255)
                                          gCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTtctctc                                                            <  1:1244264/101‑1 (MQ=255)
                                              gACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTgg                                                        >  1:259643/1‑101 (MQ=255)
                                                    cGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGcc                                                  >  1:737257/1‑101 (MQ=255)
                                                    cGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGcc                                                  <  1:3436004/101‑1 (MQ=255)
                                                    cGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGcc                                                  >  1:2578955/1‑101 (MQ=255)
                                                        tGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCtt                                              <  1:1165374/101‑1 (MQ=255)
                                                         gTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTtg                                             <  1:431301/101‑1 (MQ=255)
                                                         gTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTtg                                             >  2:797182/1‑101 (MQ=255)
                                                          ttCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTtga                                            >  1:1195215/1‑101 (MQ=255)
                                                           tCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTtgat                                           <  2:453796/101‑1 (MQ=255)
                                                             cGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTtgatga                                         <  1:4647801/101‑1 (MQ=255)
                                                              gggCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTgatgat                                        >  2:2863157/1‑101 (MQ=255)
                                                              gggCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTgatgat                                        <  2:4313016/101‑1 (MQ=255)
                                                              gggCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTgatgat                                        >  2:3900374/1‑101 (MQ=255)
                                                               ggCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGAtt                                       <  2:66071/101‑1 (MQ=255)
                                                               ggCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGAtt                                       <  1:4775140/101‑1 (MQ=255)
                                                                gCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGAttt                                      >  2:1826936/1‑101 (MQ=255)
                                                                  aGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTcc                                    <  2:2611631/101‑1 (MQ=255)
                                                                  aGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTcc                                    <  1:2294500/101‑1 (MQ=255)
                                                                   ggcggcAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCt                                   <  2:227092/101‑1 (MQ=255)
                                                                      ggcAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGaa                                >  2:730641/1‑101 (MQ=255)
                                                                       gcAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGaaa                               <  2:3076162/101‑1 (MQ=255)
                                                                       gcAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGaaa                               <  1:224071/101‑1 (MQ=255)
                                                                       gcAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGaaa                               <  2:4423847/101‑1 (MQ=255)
                                                                        cAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAg                              <  2:259123/101‑1 (MQ=255)
                                                                        cAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAg                              >  2:4676284/1‑101 (MQ=255)
                                                                        cAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAg                              >  1:3183874/1‑101 (MQ=255)
                                                                         aaaGCAGTTGTCGCATTCATAGCACTGACCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAgc                             >  1:4107138/1‑101 (MQ=255)
                                                                            gCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCTCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAgccgc                          >  2:4365051/1‑101 (MQ=255)
                                                                            gCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAgccgc                          >  1:3860816/1‑101 (MQ=255)
                                                                            gCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAgccgc                          >  2:4031598/1‑101 (MQ=255)
                                                                              aGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGccc                        >  2:627879/1‑101 (MQ=255)
                                                                              aGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGccc                        >  2:2708973/1‑101 (MQ=255)
                                                                                 tGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGt                     >  2:1120609/1‑101 (MQ=255)
                                                                                    cGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGt                  <  2:3557316/101‑1 (MQ=255)
                                                                                      cATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGc                >  1:4923658/1‑101 (MQ=255)
                                                                                       aTTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCg               >  1:4373090/1‑101 (MQ=255)
                                                                                        ttCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCgg              >  1:3116905/1‑101 (MQ=255)
                                                                                          cATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGcc            >  2:921367/1‑101 (MQ=255)
                                                                                          cATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGcc            >  1:2907952/1‑101 (MQ=255)
                                                                                          cATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGcc            >  1:1040555/1‑101 (MQ=255)
                                                                                           aTAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCt           <  2:3304785/101‑1 (MQ=255)
                                                                                           aTAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCt           <  1:2984774/101‑1 (MQ=255)
                                                                                            tAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTc          <  1:3654872/101‑1 (MQ=255)
                                                                                            tAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTc          <  1:1856570/101‑1 (MQ=255)
                                                                                            tAGCACTGACCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGGCCAGTCGTGAGGCCTc          >  1:846546/1‑101 (MQ=255)
                                                                                             aGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCg         <  2:1861395/101‑1 (MQ=255)
                                                                                             aGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCg         <  2:1380596/101‑1 (MQ=255)
                                                                                             aGCACTGACCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCg         >  2:1010725/1‑101 (MQ=255)
                                                                                              gCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCgg        >  1:1921266/1‑101 (MQ=255)
                                                                                              gCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCgg        >  1:1084000/1‑101 (MQ=255)
                                                                                                aCTGACCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCGGcc      <  1:89018/101‑1 (MQ=255)
                                                                                                   ggCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCGGCCCgc   >  1:662758/1‑101 (MQ=255)
                                                                                                   ggCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGACCCTTGATGATTTCCTGAAAGCCGCCCACGCGTGCGGCCTCGGCCAgg   >  2:3325244/1‑100 (MQ=255)
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TGCATTTCGTCAGCTCGATGCGATAGCCGCGGCCGGGGCCGTGCTTGACGATCGCCTGTTCCGGGCAGGCGGCAAAGCAGTTGTCGCATTCATAGCACTGGCCGCAGGAATAGCAGCGTTGCGCCTCGTAGCGGGCTTCTCTCGTGGTCAGCCCCTTGATGATTTCCTGAAAGCCGCCCAGTCGTGCGGCCTCGGCCAGCC  >  NC_014034/1866032‑1866232

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG